Recruitment of MAIT Cells to the Intervillous Space of the Placenta by Placenta-Derived Chemokines
The intervillous space of the placenta is a part of the fetal-maternal interface, where maternal blood enters to provide nutrients and gas exchange. Little is known about the maternal immune cells at this site, which are in direct contact with fetal tissues. We have characterized the T cell composit...
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doaj-7032c2529765417cbcaa5eb477fc783c2020-11-24T20:42:55ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-06-011010.3389/fimmu.2019.01300451343Recruitment of MAIT Cells to the Intervillous Space of the Placenta by Placenta-Derived ChemokinesMartin Solders0Martin Solders1Laia Gorchs2Eleonor Tiblad3Sebastian Gidlöf4Sebastian Gidlöf5Edwin Leeansyah6Edwin Leeansyah7Joana Dias8Johan K. Sandberg9Isabelle Magalhaes10Anna-Carin Lundell11Helen Kaipe12Helen Kaipe13Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, Stockholm, SwedenDepartment of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenDepartment of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenCenter for Fetal Medicine, Karolinska University Hospital and Department of CLINTEC, Karolinska Insitutet, Stockholm, SwedenCenter for Fetal Medicine, Karolinska University Hospital and Department of CLINTEC, Karolinska Insitutet, Stockholm, SwedenDepartment of Women's and Children's Health, Karolinska Institutet, Stockholm, SwedenCenter for Infectious Medicine, Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, SwedenProgram in Emerging Infectious Diseases, Duke-National University of Singapore Medical School, Singapore, SingaporeCenter for Infectious Medicine, Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, SwedenCenter for Infectious Medicine, Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, SwedenDepartment of Oncology/Pathology, Karolinska Institutet, Stockholm, SwedenDepartment of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenClinical Immunology and Transfusion Medicine, Karolinska University Hospital, Stockholm, SwedenDepartment of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenThe intervillous space of the placenta is a part of the fetal-maternal interface, where maternal blood enters to provide nutrients and gas exchange. Little is known about the maternal immune cells at this site, which are in direct contact with fetal tissues. We have characterized the T cell composition and chemokine profile in paired intervillous and peripheral blood samples from healthy mothers giving birth following term pregnancies. Mucosal-associated invariant T (MAIT) cells and effector memory (EM) T cells were enriched in the intervillous blood compared to peripheral blood, suggesting that MAIT cells and other EM T cells home to the placenta during pregnancy. Furthermore, pregnant women had lower proportions of peripheral blood MAIT cells compared to non-pregnant women. The levels of several chemokines were significantly higher in intervillous compared to peripheral blood, including macrophage migration inhibitory factor (MIF), CXCL10, and CCL25, whereas CCL21, CCL27 and CXCL12 were lower. Migration assays showed that MAIT cells and EM T cells migrated toward conditioned medium from placental explants. A multivariate factor analysis indicated that high levels of MIF and CCL25 were associated with high proportions of MAIT cells in intervillous blood. Blocking of MIF or a combination of MIF, CCL25, and CCL20 in migration assays inhibited MAIT cell migration toward placenta conditioned medium. Finally, MAIT cells showed migratory capacities toward recombinant MIF. Together, these findings indicate that term placental tissues attract MAIT cells, and that this effect is at least partly mediated by MIF.https://www.frontiersin.org/article/10.3389/fimmu.2019.01300/fullMAIT cellsplacentachemokinesreproductive immunologyintervillous spaceT cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Martin Solders Martin Solders Laia Gorchs Eleonor Tiblad Sebastian Gidlöf Sebastian Gidlöf Edwin Leeansyah Edwin Leeansyah Joana Dias Johan K. Sandberg Isabelle Magalhaes Anna-Carin Lundell Helen Kaipe Helen Kaipe |
spellingShingle |
Martin Solders Martin Solders Laia Gorchs Eleonor Tiblad Sebastian Gidlöf Sebastian Gidlöf Edwin Leeansyah Edwin Leeansyah Joana Dias Johan K. Sandberg Isabelle Magalhaes Anna-Carin Lundell Helen Kaipe Helen Kaipe Recruitment of MAIT Cells to the Intervillous Space of the Placenta by Placenta-Derived Chemokines Frontiers in Immunology MAIT cells placenta chemokines reproductive immunology intervillous space T cells |
author_facet |
Martin Solders Martin Solders Laia Gorchs Eleonor Tiblad Sebastian Gidlöf Sebastian Gidlöf Edwin Leeansyah Edwin Leeansyah Joana Dias Johan K. Sandberg Isabelle Magalhaes Anna-Carin Lundell Helen Kaipe Helen Kaipe |
author_sort |
Martin Solders |
title |
Recruitment of MAIT Cells to the Intervillous Space of the Placenta by Placenta-Derived Chemokines |
title_short |
Recruitment of MAIT Cells to the Intervillous Space of the Placenta by Placenta-Derived Chemokines |
title_full |
Recruitment of MAIT Cells to the Intervillous Space of the Placenta by Placenta-Derived Chemokines |
title_fullStr |
Recruitment of MAIT Cells to the Intervillous Space of the Placenta by Placenta-Derived Chemokines |
title_full_unstemmed |
Recruitment of MAIT Cells to the Intervillous Space of the Placenta by Placenta-Derived Chemokines |
title_sort |
recruitment of mait cells to the intervillous space of the placenta by placenta-derived chemokines |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2019-06-01 |
description |
The intervillous space of the placenta is a part of the fetal-maternal interface, where maternal blood enters to provide nutrients and gas exchange. Little is known about the maternal immune cells at this site, which are in direct contact with fetal tissues. We have characterized the T cell composition and chemokine profile in paired intervillous and peripheral blood samples from healthy mothers giving birth following term pregnancies. Mucosal-associated invariant T (MAIT) cells and effector memory (EM) T cells were enriched in the intervillous blood compared to peripheral blood, suggesting that MAIT cells and other EM T cells home to the placenta during pregnancy. Furthermore, pregnant women had lower proportions of peripheral blood MAIT cells compared to non-pregnant women. The levels of several chemokines were significantly higher in intervillous compared to peripheral blood, including macrophage migration inhibitory factor (MIF), CXCL10, and CCL25, whereas CCL21, CCL27 and CXCL12 were lower. Migration assays showed that MAIT cells and EM T cells migrated toward conditioned medium from placental explants. A multivariate factor analysis indicated that high levels of MIF and CCL25 were associated with high proportions of MAIT cells in intervillous blood. Blocking of MIF or a combination of MIF, CCL25, and CCL20 in migration assays inhibited MAIT cell migration toward placenta conditioned medium. Finally, MAIT cells showed migratory capacities toward recombinant MIF. Together, these findings indicate that term placental tissues attract MAIT cells, and that this effect is at least partly mediated by MIF. |
topic |
MAIT cells placenta chemokines reproductive immunology intervillous space T cells |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.01300/full |
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