SAPK10-Mediated Phosphorylation on WRKY72 Releases Its Suppression on Jasmonic Acid Biosynthesis and Bacterial Blight Resistance
Summary: Bacterial blight caused by the infection of Xanthomonas oryzae pv. oryzae (Xoo) is a devastating disease that severely challenges the yield of rice. Here, we report the identification of a “SAPK10-WRKY72-AOS1” module, through which Xoo infection stimulates the suppression of jasmonic acid (...
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2019-06-01
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doaj-7049185fd1814d1897a9dd7f7575b4c12020-11-25T01:13:04ZengElsevieriScience2589-00422019-06-0116499510SAPK10-Mediated Phosphorylation on WRKY72 Releases Its Suppression on Jasmonic Acid Biosynthesis and Bacterial Blight ResistanceYuxuan Hou0Yifeng Wang1Liqun Tang2Xiaohong Tong3Ling Wang4Lianmeng Liu5Shiwen Huang6Jian Zhang7State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China; Corresponding authorState Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China; Corresponding authorSummary: Bacterial blight caused by the infection of Xanthomonas oryzae pv. oryzae (Xoo) is a devastating disease that severely challenges the yield of rice. Here, we report the identification of a “SAPK10-WRKY72-AOS1” module, through which Xoo infection stimulates the suppression of jasmonic acid (JA) biosynthesis to cause Xoo susceptibility. WRKY72 directly binds to the W-box in the promoter of JA biosynthesis gene AOS1 and represses its transcription by inducing DNA hypermethylation on the target site, which finally led to lower endogenous JA level and higher Xoo susceptibility. Abscisic acid (ABA)-inducible SnRK2-type kinase SAPK10 phosphorylates WRKY72 at Thr 129. The SAPK10-mediated phosphorylation impairs the DNA-binding ability of WRKY72 and releases its suppression on AOS1 and JA biosynthesis. Our work highlights a module of how pathogen stimuli lead to plant susceptibility, as well as a potential pathway for ABA-JA interplay with post-translational modification and epigenetic regulation mechanism involved. : Biochemistry; Cell Biology; Genetics; Microbiology; Molecular Biology; Plant Biology Subject Areas: Biochemistry, Cell Biology, Genetics, Microbiology, Molecular Biology, Plant Biologyhttp://www.sciencedirect.com/science/article/pii/S2589004219301919 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuxuan Hou Yifeng Wang Liqun Tang Xiaohong Tong Ling Wang Lianmeng Liu Shiwen Huang Jian Zhang |
spellingShingle |
Yuxuan Hou Yifeng Wang Liqun Tang Xiaohong Tong Ling Wang Lianmeng Liu Shiwen Huang Jian Zhang SAPK10-Mediated Phosphorylation on WRKY72 Releases Its Suppression on Jasmonic Acid Biosynthesis and Bacterial Blight Resistance iScience |
author_facet |
Yuxuan Hou Yifeng Wang Liqun Tang Xiaohong Tong Ling Wang Lianmeng Liu Shiwen Huang Jian Zhang |
author_sort |
Yuxuan Hou |
title |
SAPK10-Mediated Phosphorylation on WRKY72 Releases Its Suppression on Jasmonic Acid Biosynthesis and Bacterial Blight Resistance |
title_short |
SAPK10-Mediated Phosphorylation on WRKY72 Releases Its Suppression on Jasmonic Acid Biosynthesis and Bacterial Blight Resistance |
title_full |
SAPK10-Mediated Phosphorylation on WRKY72 Releases Its Suppression on Jasmonic Acid Biosynthesis and Bacterial Blight Resistance |
title_fullStr |
SAPK10-Mediated Phosphorylation on WRKY72 Releases Its Suppression on Jasmonic Acid Biosynthesis and Bacterial Blight Resistance |
title_full_unstemmed |
SAPK10-Mediated Phosphorylation on WRKY72 Releases Its Suppression on Jasmonic Acid Biosynthesis and Bacterial Blight Resistance |
title_sort |
sapk10-mediated phosphorylation on wrky72 releases its suppression on jasmonic acid biosynthesis and bacterial blight resistance |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2019-06-01 |
description |
Summary: Bacterial blight caused by the infection of Xanthomonas oryzae pv. oryzae (Xoo) is a devastating disease that severely challenges the yield of rice. Here, we report the identification of a “SAPK10-WRKY72-AOS1” module, through which Xoo infection stimulates the suppression of jasmonic acid (JA) biosynthesis to cause Xoo susceptibility. WRKY72 directly binds to the W-box in the promoter of JA biosynthesis gene AOS1 and represses its transcription by inducing DNA hypermethylation on the target site, which finally led to lower endogenous JA level and higher Xoo susceptibility. Abscisic acid (ABA)-inducible SnRK2-type kinase SAPK10 phosphorylates WRKY72 at Thr 129. The SAPK10-mediated phosphorylation impairs the DNA-binding ability of WRKY72 and releases its suppression on AOS1 and JA biosynthesis. Our work highlights a module of how pathogen stimuli lead to plant susceptibility, as well as a potential pathway for ABA-JA interplay with post-translational modification and epigenetic regulation mechanism involved. : Biochemistry; Cell Biology; Genetics; Microbiology; Molecular Biology; Plant Biology Subject Areas: Biochemistry, Cell Biology, Genetics, Microbiology, Molecular Biology, Plant Biology |
url |
http://www.sciencedirect.com/science/article/pii/S2589004219301919 |
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