Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae)
The first barrier, when penetrating an insect host, encountered by microorganisms like fungi is insects cuticle; fungi produce a wide variety of extracellular enzymes involved in the degradation of protein, chitin, and lipids. The main objectives were to assay the enzymatic activity of Cordyceps loc...
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doaj-706b115a20664e6992220dca3f19ddb32021-05-28T05:00:09ZengElsevierJournal of King Saud University: Science1018-36472021-06-01334101411Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae)Sebastian A. Pelizza0Natalia A. Ferreri1Lorena A. Elíades2Betina Galarza3Marta N. Cabello4María L. Russo5Florencia Vianna6Ana C. Scorsetti7Carlos E. Lange8Instituto de Botánica Carlos Spegazzini (FCNyM-UNLP), Calle 53 # 477, La Plata 1900, Argentina; Corresponding author.Instituto de Botánica Carlos Spegazzini (FCNyM-UNLP), Calle 53 # 477, La Plata 1900, ArgentinaInstituto de Botánica Carlos Spegazzini (FCNyM-UNLP), Calle 53 # 477, La Plata 1900, ArgentinaCentro de Investigaciones de Tecnología del Cuero (CITEC), ArgentinaInstituto de Botánica Carlos Spegazzini (FCNyM-UNLP), Calle 53 # 477, La Plata 1900, Argentina; Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA), ArgentinaInstituto de Botánica Carlos Spegazzini (FCNyM-UNLP), Calle 53 # 477, La Plata 1900, ArgentinaInstituto de Botánica Carlos Spegazzini (FCNyM-UNLP), Calle 53 # 477, La Plata 1900, ArgentinaInstituto de Botánica Carlos Spegazzini (FCNyM-UNLP), Calle 53 # 477, La Plata 1900, ArgentinaCentro de Estudios Parasitológicos y de Vectores (CEPAVE), CCT La Plata-CONICET-UNLP, Boulevard 120 s/n entre Av. 60 y Calle 64, La Plata 1900, Argentina; Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA), ArgentinaThe first barrier, when penetrating an insect host, encountered by microorganisms like fungi is insects cuticle; fungi produce a wide variety of extracellular enzymes involved in the degradation of protein, chitin, and lipids. The main objectives were to assay the enzymatic activity of Cordyceps locustiphila anamorph, in solid and liquid medium at different temperatures and also to evaluate the pathogenicity against the South American locust, Schistocerca cancellata. Conidia of C. locustiphila anamorph were adjusted to 1 × 104, 1 × 106 and 1 × 108 conidia/ml and mortality was recorded for S. cancellata nymphs. The enzymatic activities were determined through a plate test and liquid medium. When assessing the pathogenicity, the fungus caused the highest mortality (84.5 ± 3.5%) at a dose of 1 × 108 conidia/ml. The proteolytic activity showed the highest values (1.56 ± 0.21) (U) at 26 °C. The highest lipolytic activity (1.13 ± 0.36) (U) was observed at 26 °C, while the highest chitinolytic activity was of 0.85 ± 0.1 (U) at 4 °C. Significant differences were observed for enzymatic production on liquid medium, the highest values were recorded for chitinolytic activity (1.63 ± 0.04) (U) and the lowest for caseinolytic activity (0.04 ± 0.001) (U). The results obtained on mortality as well as the quality and variety of enzymes produced by C. locustiphila suggest that this fungus has features that make it a good candidate to be used as a biological agent for the control of the South American locust.http://www.sciencedirect.com/science/article/pii/S1018364721000720Biological controlEntomopathogenic fungiEnzyme productionMortalityPest insect |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sebastian A. Pelizza Natalia A. Ferreri Lorena A. Elíades Betina Galarza Marta N. Cabello María L. Russo Florencia Vianna Ana C. Scorsetti Carlos E. Lange |
spellingShingle |
Sebastian A. Pelizza Natalia A. Ferreri Lorena A. Elíades Betina Galarza Marta N. Cabello María L. Russo Florencia Vianna Ana C. Scorsetti Carlos E. Lange Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae) Journal of King Saud University: Science Biological control Entomopathogenic fungi Enzyme production Mortality Pest insect |
author_facet |
Sebastian A. Pelizza Natalia A. Ferreri Lorena A. Elíades Betina Galarza Marta N. Cabello María L. Russo Florencia Vianna Ana C. Scorsetti Carlos E. Lange |
author_sort |
Sebastian A. Pelizza |
title |
Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae) |
title_short |
Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae) |
title_full |
Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae) |
title_fullStr |
Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae) |
title_full_unstemmed |
Enzymatic activity and virulence of Cordyceps locustiphila (Hypocreales: Cordycipitaceae) on the South American locust Schistocerca cancellata (Orthoptera: Acrididae) |
title_sort |
enzymatic activity and virulence of cordyceps locustiphila (hypocreales: cordycipitaceae) on the south american locust schistocerca cancellata (orthoptera: acrididae) |
publisher |
Elsevier |
series |
Journal of King Saud University: Science |
issn |
1018-3647 |
publishDate |
2021-06-01 |
description |
The first barrier, when penetrating an insect host, encountered by microorganisms like fungi is insects cuticle; fungi produce a wide variety of extracellular enzymes involved in the degradation of protein, chitin, and lipids. The main objectives were to assay the enzymatic activity of Cordyceps locustiphila anamorph, in solid and liquid medium at different temperatures and also to evaluate the pathogenicity against the South American locust, Schistocerca cancellata. Conidia of C. locustiphila anamorph were adjusted to 1 × 104, 1 × 106 and 1 × 108 conidia/ml and mortality was recorded for S. cancellata nymphs. The enzymatic activities were determined through a plate test and liquid medium. When assessing the pathogenicity, the fungus caused the highest mortality (84.5 ± 3.5%) at a dose of 1 × 108 conidia/ml. The proteolytic activity showed the highest values (1.56 ± 0.21) (U) at 26 °C. The highest lipolytic activity (1.13 ± 0.36) (U) was observed at 26 °C, while the highest chitinolytic activity was of 0.85 ± 0.1 (U) at 4 °C. Significant differences were observed for enzymatic production on liquid medium, the highest values were recorded for chitinolytic activity (1.63 ± 0.04) (U) and the lowest for caseinolytic activity (0.04 ± 0.001) (U). The results obtained on mortality as well as the quality and variety of enzymes produced by C. locustiphila suggest that this fungus has features that make it a good candidate to be used as a biological agent for the control of the South American locust. |
topic |
Biological control Entomopathogenic fungi Enzyme production Mortality Pest insect |
url |
http://www.sciencedirect.com/science/article/pii/S1018364721000720 |
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