Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research
Abstract The lack of reproducibility of animal experimental results between laboratories, particularly in studies investigating the microbiota, has raised concern among the scientific community. Factors such as environment, stress and sex have been identified as contributors, whereas dietary composi...
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doaj-708e388632bb4a2ca819b845f223579c2020-12-08T11:30:47ZengNature Publishing GroupScientific Reports2045-23222020-10-0110111310.1038/s41598-020-74460-8Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical researchC. J. Tuck0G. De Palma1K. Takami2B. Brant3A. Caminero4D. E. Reed5J. G. Muir6P. R. Gibson7A. Winterborn8E. F. Verdu9P. Bercik10S. Vanner11Gastrointestinal Diseases Research Unit, Kingston General Hospital, Queen’s UniversityFarncombe Institute, McMaster UniversityGastrointestinal Diseases Research Unit, Kingston General Hospital, Queen’s UniversityGastrointestinal Diseases Research Unit, Kingston General Hospital, Queen’s UniversityFarncombe Institute, McMaster UniversityGastrointestinal Diseases Research Unit, Kingston General Hospital, Queen’s UniversityDepartment of Gastroenterology, Monash UniversityDepartment of Gastroenterology, Monash UniversityFaculty of Health Sciences, Queen’s UniversityFarncombe Institute, McMaster UniversityFarncombe Institute, McMaster UniversityGastrointestinal Diseases Research Unit, Kingston General Hospital, Queen’s UniversityAbstract The lack of reproducibility of animal experimental results between laboratories, particularly in studies investigating the microbiota, has raised concern among the scientific community. Factors such as environment, stress and sex have been identified as contributors, whereas dietary composition has received less attention. This study firstly evaluated the use of commercially available rodent diets across research institutions, with 28 different diets reported by 45 survey respondents. Secondly, highly variable ingredient, FODMAP (Fermentable Oligo-, Di-, Mono-saccharides And Polyols) and gluten content was found between different commercially available rodent diets. Finally, 40 mice were randomized to four groups, each receiving a different commercially available rodent diet, and the dietary impact on cecal microbiota, short- and branched-chain fatty acid profiles was evaluated. The gut microbiota composition differed significantly between diets and sexes, with significantly different clusters in β-diversity. Total BCFA were highest (p = 0.01) and SCFA were lowest (p = 0.03) in mice fed a diet lower in FODMAPs and gluten. These results suggest that nutritional composition of commercially available rodent diets impact gut microbiota profiles and fermentation patterns, with major implications for the reproducibility of results across laboratories. However, further studies are required to elucidate the specific dietary factors driving these changes.https://doi.org/10.1038/s41598-020-74460-8 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
C. J. Tuck G. De Palma K. Takami B. Brant A. Caminero D. E. Reed J. G. Muir P. R. Gibson A. Winterborn E. F. Verdu P. Bercik S. Vanner |
spellingShingle |
C. J. Tuck G. De Palma K. Takami B. Brant A. Caminero D. E. Reed J. G. Muir P. R. Gibson A. Winterborn E. F. Verdu P. Bercik S. Vanner Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research Scientific Reports |
author_facet |
C. J. Tuck G. De Palma K. Takami B. Brant A. Caminero D. E. Reed J. G. Muir P. R. Gibson A. Winterborn E. F. Verdu P. Bercik S. Vanner |
author_sort |
C. J. Tuck |
title |
Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research |
title_short |
Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research |
title_full |
Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research |
title_fullStr |
Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research |
title_full_unstemmed |
Nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research |
title_sort |
nutritional profile of rodent diets impacts experimental reproducibility in microbiome preclinical research |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2020-10-01 |
description |
Abstract The lack of reproducibility of animal experimental results between laboratories, particularly in studies investigating the microbiota, has raised concern among the scientific community. Factors such as environment, stress and sex have been identified as contributors, whereas dietary composition has received less attention. This study firstly evaluated the use of commercially available rodent diets across research institutions, with 28 different diets reported by 45 survey respondents. Secondly, highly variable ingredient, FODMAP (Fermentable Oligo-, Di-, Mono-saccharides And Polyols) and gluten content was found between different commercially available rodent diets. Finally, 40 mice were randomized to four groups, each receiving a different commercially available rodent diet, and the dietary impact on cecal microbiota, short- and branched-chain fatty acid profiles was evaluated. The gut microbiota composition differed significantly between diets and sexes, with significantly different clusters in β-diversity. Total BCFA were highest (p = 0.01) and SCFA were lowest (p = 0.03) in mice fed a diet lower in FODMAPs and gluten. These results suggest that nutritional composition of commercially available rodent diets impact gut microbiota profiles and fermentation patterns, with major implications for the reproducibility of results across laboratories. However, further studies are required to elucidate the specific dietary factors driving these changes. |
url |
https://doi.org/10.1038/s41598-020-74460-8 |
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