Effect of Maternal Diet and Milk Lipid Composition on the Infant Gut and Maternal Milk Microbiomes
Inter-subject variability in human milk microbiome is well known; however, its origins and possible relationship to the mother’s diet are still debated. We investigated associations between maternal nutrition, milk fatty acids composition and microbiomes in mother–infant dyads. Breast milk and infan...
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doaj-70ae2e8cd4944a7fa3fd2b4aa6d19cc82020-11-25T03:40:47ZengMDPI AGNutrients2072-66432020-08-01122539253910.3390/nu12092539Effect of Maternal Diet and Milk Lipid Composition on the Infant Gut and Maternal Milk MicrobiomesMichal Dayagi Babakobi0Leah Reshef1Shalev Gihaz2Bogdan Belgorodsky3Ayelet Fishman4Yoram Bujanover5Uri Gophna6The Shmunis School of Biomedicine and Cancer Research, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 6900001, IsraelThe Shmunis School of Biomedicine and Cancer Research, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 6900001, IsraelDepartment of Biotechnology and Food Engineering, Technion- Israel Institute of Technology, Haifa 3200003, IsraelSchool of Chemistry, Tel Aviv University, Tel Aviv 69000, IsraelDepartment of Biotechnology and Food Engineering, Technion- Israel Institute of Technology, Haifa 3200003, IsraelSafra Children’s Hospital, Sheba Medical Center, Ramat Gan 52621, IsraelThe Shmunis School of Biomedicine and Cancer Research, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 6900001, IsraelInter-subject variability in human milk microbiome is well known; however, its origins and possible relationship to the mother’s diet are still debated. We investigated associations between maternal nutrition, milk fatty acids composition and microbiomes in mother–infant dyads. Breast milk and infant fecal samples were collected across three time points (one week, one month and three months postpartum) from 22 mother–infant pairs. Food frequency questionnaires for the months of pregnancy and three months postpartum were collected. Milk fatty acids were analyzed by GC–MS and the microbiome in breast milk and infant feces was determined by 16S rRNA sequencing. Statistical interactions were computed using Spearman’s method and corrected for multiple comparisons. We found significant negative correlation between <i>Streptococcus</i> relative abundance in maternal milk and intake of unsaturated fatty acids and folic acid at one month postpartum. At three months postpartum, vitamin B-12 consumption was significantly associated with a single operational taxonomic unit belonging to <i>Streptococcus</i>. Comparison between milk microbiome and lipid composition showed, one-month postpartum, significant negative correlation between <i>Streptococcus</i> relative abundance and the abundance of oleic acid. Additional correlations were detected between <i>Staphylococcus hominis</i> and two medium-chain saturated fatty acids. Our results reinforce the hypothesis that maternal nutrition may affect milk microbiome.https://www.mdpi.com/2072-6643/12/9/2539breast milkhuman milk microbiomehuman milk fat compositioninfant gut microbiomematernal diet |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michal Dayagi Babakobi Leah Reshef Shalev Gihaz Bogdan Belgorodsky Ayelet Fishman Yoram Bujanover Uri Gophna |
spellingShingle |
Michal Dayagi Babakobi Leah Reshef Shalev Gihaz Bogdan Belgorodsky Ayelet Fishman Yoram Bujanover Uri Gophna Effect of Maternal Diet and Milk Lipid Composition on the Infant Gut and Maternal Milk Microbiomes Nutrients breast milk human milk microbiome human milk fat composition infant gut microbiome maternal diet |
author_facet |
Michal Dayagi Babakobi Leah Reshef Shalev Gihaz Bogdan Belgorodsky Ayelet Fishman Yoram Bujanover Uri Gophna |
author_sort |
Michal Dayagi Babakobi |
title |
Effect of Maternal Diet and Milk Lipid Composition on the Infant Gut and Maternal Milk Microbiomes |
title_short |
Effect of Maternal Diet and Milk Lipid Composition on the Infant Gut and Maternal Milk Microbiomes |
title_full |
Effect of Maternal Diet and Milk Lipid Composition on the Infant Gut and Maternal Milk Microbiomes |
title_fullStr |
Effect of Maternal Diet and Milk Lipid Composition on the Infant Gut and Maternal Milk Microbiomes |
title_full_unstemmed |
Effect of Maternal Diet and Milk Lipid Composition on the Infant Gut and Maternal Milk Microbiomes |
title_sort |
effect of maternal diet and milk lipid composition on the infant gut and maternal milk microbiomes |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2020-08-01 |
description |
Inter-subject variability in human milk microbiome is well known; however, its origins and possible relationship to the mother’s diet are still debated. We investigated associations between maternal nutrition, milk fatty acids composition and microbiomes in mother–infant dyads. Breast milk and infant fecal samples were collected across three time points (one week, one month and three months postpartum) from 22 mother–infant pairs. Food frequency questionnaires for the months of pregnancy and three months postpartum were collected. Milk fatty acids were analyzed by GC–MS and the microbiome in breast milk and infant feces was determined by 16S rRNA sequencing. Statistical interactions were computed using Spearman’s method and corrected for multiple comparisons. We found significant negative correlation between <i>Streptococcus</i> relative abundance in maternal milk and intake of unsaturated fatty acids and folic acid at one month postpartum. At three months postpartum, vitamin B-12 consumption was significantly associated with a single operational taxonomic unit belonging to <i>Streptococcus</i>. Comparison between milk microbiome and lipid composition showed, one-month postpartum, significant negative correlation between <i>Streptococcus</i> relative abundance and the abundance of oleic acid. Additional correlations were detected between <i>Staphylococcus hominis</i> and two medium-chain saturated fatty acids. Our results reinforce the hypothesis that maternal nutrition may affect milk microbiome. |
topic |
breast milk human milk microbiome human milk fat composition infant gut microbiome maternal diet |
url |
https://www.mdpi.com/2072-6643/12/9/2539 |
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