Identification of QTLs for rice yield and yield-related traits using high density SNPs linkage map

• Main Authors: M Ahmadzadeh, N Babaian, Qasem Mohammadi Nezhad, N Bagheri, R Komar
• Format: Article
• Language: fas
• Published: Shahid Bahonar University of Kerman 2017-10-01
• Series: مجله بیوتکنولوژی کشاورزی
• Subjects: mapping; snp marker; quantitative traits; rice
• Online Access: https://jab.uk.ac.ir/article_1876_0e4f307a62210260afbc6da378c79683.pdf
• ISSN: 2228-6705; 2228-6500

<span style="font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';"><span style="font-family: Times New Roman;">Rice is one of the most important cereal with great variation, which is a major food for more than half of the world’s population. Mapping quantitative traits loci is one of the applied and momentous approaches to study yield-related traits. In present study 188 F₄ rice lines derived from CSR28 and Sadri cross along with the parents were used for genotyping and phenotyping. The objective of this study was to construct high saturation linkage map using SNPs markers (Infinium Illumina 6K SNP chip) and QTL identification of yield-related traits in 188 F4 population. Mapping of quantitative traits loci led to identify 21</span></span><span style="font-family: Times New Roman;"><span style="font-size: 12pt; mso-bidi-language: FA; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';">QTLs for studied traits on chromosomes 1, 2, 3, 5, 7, 8 and 10. One QTL (</span><em><span style="color: black; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';">qFG_N-3-1</span></em><span style="color: black; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';">) was identified for filled grain number per plant on chromosome 3. For spikelet fertility, three </span><span style="color: black; font-size: 12pt; mso-bidi-language: FA; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';">QTLs (</span><em><span style="color: black; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';">qSpkF_N-2-1</span></em><span style="color: black; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';">, <em>qSpkF_N-3-1</em></span></span><span style="color: black; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';"><span style="font-family: Times New Roman;">and</span></span><span style="font-family: Times New Roman;"><em><span style="color: black; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';">qSpkF_N-5-1</span></em><span style="color: black; font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';">) were mapped on chromosomes 2, 3 and 5, which explained 29.28 percentage of phenotypic variation. One QTL (<em>qGY_N-8-1</em>) was detected for grain yield on chromosome 8, which was flanked by 9037125 and 9049928 markers. In conclusion, besides the </span><span style="font-size: 12pt; mso-bidi-language: FA; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';">QTLs which is reported for first time, the QTLs</span><span style="font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus';"> were consistent as reported previously, could be introduced as reliable QTLs that is suitable for </span><span style="font-size: 12pt; mso-bidi-font-size: 14.0pt; mso-bidi-font-family: 'B Lotus'; mso-fareast-font-family: AGaramondPro-Regular;">marker assisted breeding programs.</span></span>