Pathogenicity Levels of Colombian Strains of <i>Candida auris</i> and Brazilian Strains of <i>Candida haemulonii</i> Species Complex in Both Murine and <i>Galleria mellonella</i> Experimental Models
<i>Candida auris</i> and <i>Candida haemulonii</i> complex (<i>C. haemulonii, C. haemulonii</i> var. <i>vulnera</i> and <i>C. duobushaemulonii</i>) are phylogenetically related species that share some physiological features and habits. In t...
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doaj-7102f263fa7641e9afbb8394022050292020-11-25T03:45:21ZengMDPI AGJournal of Fungi2309-608X2020-07-01610410410.3390/jof6030104Pathogenicity Levels of Colombian Strains of <i>Candida auris</i> and Brazilian Strains of <i>Candida haemulonii</i> Species Complex in Both Murine and <i>Galleria mellonella</i> Experimental ModelsJulián E. Muñoz0Laura M. Ramirez1Lucas dos Santos Dias2Laura A. Rivas3Lívia S. Ramos4André L. S. Santos5Carlos P. Taborda6Claudia M. Parra-Giraldo7MICROS Group, Medicine Traslacional Institute, School of Medicine and Health Sciences, Universidad del Rosario, Bogotá, D.C. 111221, ColombiaUnidad de Proteómica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C. 110231, ColombiaDepartment of Microbiology, Biomedical Sciences Institute, University of São Paulo (USP), São Paulo, SP 05508-060, BrazilUnidad de Proteómica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C. 110231, ColombiaLaboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes (IMPG), Centro de Ciências da Saúde (CCS), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-901, BrazilLaboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes (IMPG), Centro de Ciências da Saúde (CCS), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-901, BrazilDepartment of Microbiology, Biomedical Sciences Institute, University of São Paulo (USP), São Paulo, SP 05508-060, BrazilUnidad de Proteómica y Micosis Humanas, Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C. 110231, Colombia<i>Candida auris</i> and <i>Candida haemulonii</i> complex (<i>C. haemulonii, C. haemulonii</i> var. <i>vulnera</i> and <i>C. duobushaemulonii</i>) are phylogenetically related species that share some physiological features and habits. In the present study, we compared the virulence of these yeast species using two different experimental models: (i) <i>Galleria mellonella</i> larvae to evaluate the survival rate, fungal burden, histopathology and phagocytosis index and (ii) BALB/c mice to evaluate the survival. In addition, the fungal capacity to form biofilm over an inert surface was analyzed. Our results showed that in both experimental models, the animal survival rate was lower when infected with <i>C. auris</i> strains than the <i>C. haemulonii</i> species complex. The hemocytes of <i>G. mellonella</i> showed a significantly reduced ability to phagocytize the most virulent strains forming the <i>C. haemulonii</i> species complex. Interestingly, for <i>C. auris</i>, it was impossible to measure the phagocytosis index due to a general lysis of the hemocytes. Moreover, it was observed a greater capability of biofilm formation by <i>C. auris</i> compared to <i>C. haemulonii</i> species complex. In conclusion, we observed that <i>C. auris</i> and <i>C.</i> <i>haemulonii</i> complex have different levels of pathogenicity in the experimental models employed in the present study.https://www.mdpi.com/2309-608X/6/3/104<i>Candida auris</i><i>Candida haemulonii</i> species complexvirulencecandidiasisbiofilm<i>Galleria mellonella</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julián E. Muñoz Laura M. Ramirez Lucas dos Santos Dias Laura A. Rivas Lívia S. Ramos André L. S. Santos Carlos P. Taborda Claudia M. Parra-Giraldo |
spellingShingle |
Julián E. Muñoz Laura M. Ramirez Lucas dos Santos Dias Laura A. Rivas Lívia S. Ramos André L. S. Santos Carlos P. Taborda Claudia M. Parra-Giraldo Pathogenicity Levels of Colombian Strains of <i>Candida auris</i> and Brazilian Strains of <i>Candida haemulonii</i> Species Complex in Both Murine and <i>Galleria mellonella</i> Experimental Models Journal of Fungi <i>Candida auris</i> <i>Candida haemulonii</i> species complex virulence candidiasis biofilm <i>Galleria mellonella</i> |
author_facet |
Julián E. Muñoz Laura M. Ramirez Lucas dos Santos Dias Laura A. Rivas Lívia S. Ramos André L. S. Santos Carlos P. Taborda Claudia M. Parra-Giraldo |
author_sort |
Julián E. Muñoz |
title |
Pathogenicity Levels of Colombian Strains of <i>Candida auris</i> and Brazilian Strains of <i>Candida haemulonii</i> Species Complex in Both Murine and <i>Galleria mellonella</i> Experimental Models |
title_short |
Pathogenicity Levels of Colombian Strains of <i>Candida auris</i> and Brazilian Strains of <i>Candida haemulonii</i> Species Complex in Both Murine and <i>Galleria mellonella</i> Experimental Models |
title_full |
Pathogenicity Levels of Colombian Strains of <i>Candida auris</i> and Brazilian Strains of <i>Candida haemulonii</i> Species Complex in Both Murine and <i>Galleria mellonella</i> Experimental Models |
title_fullStr |
Pathogenicity Levels of Colombian Strains of <i>Candida auris</i> and Brazilian Strains of <i>Candida haemulonii</i> Species Complex in Both Murine and <i>Galleria mellonella</i> Experimental Models |
title_full_unstemmed |
Pathogenicity Levels of Colombian Strains of <i>Candida auris</i> and Brazilian Strains of <i>Candida haemulonii</i> Species Complex in Both Murine and <i>Galleria mellonella</i> Experimental Models |
title_sort |
pathogenicity levels of colombian strains of <i>candida auris</i> and brazilian strains of <i>candida haemulonii</i> species complex in both murine and <i>galleria mellonella</i> experimental models |
publisher |
MDPI AG |
series |
Journal of Fungi |
issn |
2309-608X |
publishDate |
2020-07-01 |
description |
<i>Candida auris</i> and <i>Candida haemulonii</i> complex (<i>C. haemulonii, C. haemulonii</i> var. <i>vulnera</i> and <i>C. duobushaemulonii</i>) are phylogenetically related species that share some physiological features and habits. In the present study, we compared the virulence of these yeast species using two different experimental models: (i) <i>Galleria mellonella</i> larvae to evaluate the survival rate, fungal burden, histopathology and phagocytosis index and (ii) BALB/c mice to evaluate the survival. In addition, the fungal capacity to form biofilm over an inert surface was analyzed. Our results showed that in both experimental models, the animal survival rate was lower when infected with <i>C. auris</i> strains than the <i>C. haemulonii</i> species complex. The hemocytes of <i>G. mellonella</i> showed a significantly reduced ability to phagocytize the most virulent strains forming the <i>C. haemulonii</i> species complex. Interestingly, for <i>C. auris</i>, it was impossible to measure the phagocytosis index due to a general lysis of the hemocytes. Moreover, it was observed a greater capability of biofilm formation by <i>C. auris</i> compared to <i>C. haemulonii</i> species complex. In conclusion, we observed that <i>C. auris</i> and <i>C.</i> <i>haemulonii</i> complex have different levels of pathogenicity in the experimental models employed in the present study. |
topic |
<i>Candida auris</i> <i>Candida haemulonii</i> species complex virulence candidiasis biofilm <i>Galleria mellonella</i> |
url |
https://www.mdpi.com/2309-608X/6/3/104 |
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