CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii

CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii

Summary: Infections caused by drug-resistant Acinetobacter baumannii have posed a serious threat to global public health. However, genetic manipulation methods, the primary way to study pathogenesis and drug-resistance mechanisms, remain time consuming and inefficient. Here, we provide a detailed pr...

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Main Authors: Yu Wang, Zhipeng Wang, Quanjiang Ji
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300125
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spelling doaj-7103d16500ff4e57897dcde64a53c3522020-11-25T04:01:29ZengElsevierSTAR Protocols2666-16672020-06-0111100025CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumanniiYu Wang0Zhipeng Wang1Quanjiang Ji2School of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China; College of Life Science and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China; Corresponding authorSchool of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, ChinaSchool of Physical Science and Technology, ShanghaiTech University, Shanghai 201210, China; Corresponding authorSummary: Infections caused by drug-resistant Acinetobacter baumannii have posed a serious threat to global public health. However, genetic manipulation methods, the primary way to study pathogenesis and drug-resistance mechanisms, remain time consuming and inefficient. Here, we provide a detailed protocol for genetic manipulation, including gene deletion, insertion, and point mutation in A. baumannii using the platform.For complete details on the use and execution of this protocol, please refer to Wang et al. (2019).http://www.sciencedirect.com/science/article/pii/S2666166720300125
collection DOAJ
language English
format Article
sources DOAJ
author Yu Wang
Zhipeng Wang
Quanjiang Ji
spellingShingle Yu Wang
Zhipeng Wang
Quanjiang Ji
CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii
STAR Protocols
author_facet Yu Wang
Zhipeng Wang
Quanjiang Ji
author_sort Yu Wang
title CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii
title_short CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii
title_full CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii
title_fullStr CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii
title_full_unstemmed CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii
title_sort crispr-cas9-based genome editing and cytidine base editing in acinetobacter baumannii
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-06-01
description Summary: Infections caused by drug-resistant Acinetobacter baumannii have posed a serious threat to global public health. However, genetic manipulation methods, the primary way to study pathogenesis and drug-resistance mechanisms, remain time consuming and inefficient. Here, we provide a detailed protocol for genetic manipulation, including gene deletion, insertion, and point mutation in A. baumannii using the platform.For complete details on the use and execution of this protocol, please refer to Wang et al. (2019).
url http://www.sciencedirect.com/science/article/pii/S2666166720300125
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AT zhipengwang crisprcas9basedgenomeeditingandcytidinebaseeditinginacinetobacterbaumannii
AT quanjiangji crisprcas9basedgenomeeditingandcytidinebaseeditinginacinetobacterbaumannii
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