Characterization of the Maternally Derived Antibody Immunity against Rhdv-2 after Administration in Breeding Does of an Inactivated Vaccine
Inactivated strain-specific vaccines have been successfully used to control rabbit haemorrhagic disease (RHD) caused by RHDV-2 in the rabbit industry. It is unknown whether and how vaccination of breeding does contributed to protect the population of young susceptible rabbit kits. The present study...
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doaj-71155c7d4add456ea752eb8bf91588272020-11-25T03:49:55ZengMDPI AGVaccines2076-393X2020-08-01848448410.3390/vaccines8030484Characterization of the Maternally Derived Antibody Immunity against Rhdv-2 after Administration in Breeding Does of an Inactivated VaccineMassimiliano Baratelli0Joan Molist-Badiola1Alba Puigredon-Fontanet2Mariam Pascual3Oriol Boix4Francesc Xavier Mora-Igual5Michelle Woodward6Antonio Lavazza7Lorenzo Capucci8HIPRA, 17170 Amer, SpainHIPRA, 17170 Amer, SpainHIPRA, 17170 Amer, SpainInstitute of Agrifood Research and Technology (IRTA), 08140 Barcelona, SpainHIPRA, 17170 Amer, SpainAsvet Veterinaris, 08410 Barcelona, SpainHIPRA, 17170 Amer, SpainIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna (IZSLER), 25124 Brescia, ItalyIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna (IZSLER), 25124 Brescia, ItalyInactivated strain-specific vaccines have been successfully used to control rabbit haemorrhagic disease (RHD) caused by RHDV-2 in the rabbit industry. It is unknown whether and how vaccination of breeding does contributed to protect the population of young susceptible rabbit kits. The present study investigates whether the immunity against RHDV-2 produced by vaccination of breeding does is transmitted to their progeny and its dynamic once inherited by kits. For this purpose, New Zealand female rabbits of 8–9 weeks of age were allocated into 2 groups of 40 subjects each and bred during 6 reproductive cycles. The first experimental group was vaccinated with a commercially available inactivated vaccine against RHDV-2 whereas the second group was inoculated with PBS. Moreover, the present study was also meant to identify the mechanisms of transmission of that maternal immunity. For this reason, rabbit kits of vaccinated and non-vaccinated breeding does were cross-fostered before milk uptake. The RHDV-2 antibody response was monitored in the blood serum of breeding does and of their kits by competition ELISA (cELISA) and solid-phase ELISA (spELISA). Since it has been clearly demonstrated that cELISA positive rabbits are protected from RHD, we avoided the resorting of the challenge of the kits with RHDV-2. Results showed that RHDV-2 antibodies were inherited by kits up to one year from vaccination of breeding does. Once inherited, the maternally derived antibody response against RHDV-2 lasted at least until 28 days of life. Finally, the study also elucidated that the major contribution to the maternal derived immunity against RHDV-2 in kits was provided during gestation and probably transmitted through transplacental mechanisms although lactation provided a little contribution to it. The present study contributed to elucidate the characteristics of the maternal antibody immunity produced by vaccination and its mechanisms of transmission.https://www.mdpi.com/2076-393X/8/3/484RHDV-2inactivated vaccinesmaternal derived immunityrabbits |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Massimiliano Baratelli Joan Molist-Badiola Alba Puigredon-Fontanet Mariam Pascual Oriol Boix Francesc Xavier Mora-Igual Michelle Woodward Antonio Lavazza Lorenzo Capucci |
spellingShingle |
Massimiliano Baratelli Joan Molist-Badiola Alba Puigredon-Fontanet Mariam Pascual Oriol Boix Francesc Xavier Mora-Igual Michelle Woodward Antonio Lavazza Lorenzo Capucci Characterization of the Maternally Derived Antibody Immunity against Rhdv-2 after Administration in Breeding Does of an Inactivated Vaccine Vaccines RHDV-2 inactivated vaccines maternal derived immunity rabbits |
author_facet |
Massimiliano Baratelli Joan Molist-Badiola Alba Puigredon-Fontanet Mariam Pascual Oriol Boix Francesc Xavier Mora-Igual Michelle Woodward Antonio Lavazza Lorenzo Capucci |
author_sort |
Massimiliano Baratelli |
title |
Characterization of the Maternally Derived Antibody Immunity against Rhdv-2 after Administration in Breeding Does of an Inactivated Vaccine |
title_short |
Characterization of the Maternally Derived Antibody Immunity against Rhdv-2 after Administration in Breeding Does of an Inactivated Vaccine |
title_full |
Characterization of the Maternally Derived Antibody Immunity against Rhdv-2 after Administration in Breeding Does of an Inactivated Vaccine |
title_fullStr |
Characterization of the Maternally Derived Antibody Immunity against Rhdv-2 after Administration in Breeding Does of an Inactivated Vaccine |
title_full_unstemmed |
Characterization of the Maternally Derived Antibody Immunity against Rhdv-2 after Administration in Breeding Does of an Inactivated Vaccine |
title_sort |
characterization of the maternally derived antibody immunity against rhdv-2 after administration in breeding does of an inactivated vaccine |
publisher |
MDPI AG |
series |
Vaccines |
issn |
2076-393X |
publishDate |
2020-08-01 |
description |
Inactivated strain-specific vaccines have been successfully used to control rabbit haemorrhagic disease (RHD) caused by RHDV-2 in the rabbit industry. It is unknown whether and how vaccination of breeding does contributed to protect the population of young susceptible rabbit kits. The present study investigates whether the immunity against RHDV-2 produced by vaccination of breeding does is transmitted to their progeny and its dynamic once inherited by kits. For this purpose, New Zealand female rabbits of 8–9 weeks of age were allocated into 2 groups of 40 subjects each and bred during 6 reproductive cycles. The first experimental group was vaccinated with a commercially available inactivated vaccine against RHDV-2 whereas the second group was inoculated with PBS. Moreover, the present study was also meant to identify the mechanisms of transmission of that maternal immunity. For this reason, rabbit kits of vaccinated and non-vaccinated breeding does were cross-fostered before milk uptake. The RHDV-2 antibody response was monitored in the blood serum of breeding does and of their kits by competition ELISA (cELISA) and solid-phase ELISA (spELISA). Since it has been clearly demonstrated that cELISA positive rabbits are protected from RHD, we avoided the resorting of the challenge of the kits with RHDV-2. Results showed that RHDV-2 antibodies were inherited by kits up to one year from vaccination of breeding does. Once inherited, the maternally derived antibody response against RHDV-2 lasted at least until 28 days of life. Finally, the study also elucidated that the major contribution to the maternal derived immunity against RHDV-2 in kits was provided during gestation and probably transmitted through transplacental mechanisms although lactation provided a little contribution to it. The present study contributed to elucidate the characteristics of the maternal antibody immunity produced by vaccination and its mechanisms of transmission. |
topic |
RHDV-2 inactivated vaccines maternal derived immunity rabbits |
url |
https://www.mdpi.com/2076-393X/8/3/484 |
work_keys_str_mv |
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