Parameters optimization for enzymatic assays using experimental design

The conditions for maximization enzymatic activity were determined using experimental design and inulinase from Kluyveromyces marxianus ATCC 16045. The effects of substrate concentration (sucrose and inulin), pH and temperature on inulinase activity were verified using four factorial design and surf...

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Bibliographic Details
Main Authors: J. F. M. Burkert, S. J. Kalil, F. Maugeri Filho, M. I. Rodrigues
Format: Article
Language:English
Published: Brazilian Society of Chemical Engineering 2006-06-01
Series:Brazilian Journal of Chemical Engineering
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322006000200002

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