Association Between Oral Microbiota and Cigarette Smoking in the Chinese Population
The oral microbiota has been observed to be influenced by cigarette smoking and linked to several human diseases. However, research on the effect of cigarette smoking on the oral microbiota has not been systematically conducted in the Chinese population. We profiled the oral microbiota of 316 health...
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Frontiers Media S.A.
2021-05-01
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Series: | Frontiers in Cellular and Infection Microbiology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.658203/full |
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doaj-712783e35f3e4413a5c432a08cc75134 |
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record_format |
Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yi-Jing Jia Yi-Jing Jia Ying Liao Yong-Qiao He Mei-Qi Zheng Xia-Ting Tong Xia-Ting Tong Wen-Qiong Xue Jiang-Bo Zhang Lei-Lei Yuan Lei-Lei Yuan Wen-Li Zhang Wei-Hua Jia Wei-Hua Jia |
spellingShingle |
Yi-Jing Jia Yi-Jing Jia Ying Liao Yong-Qiao He Mei-Qi Zheng Xia-Ting Tong Xia-Ting Tong Wen-Qiong Xue Jiang-Bo Zhang Lei-Lei Yuan Lei-Lei Yuan Wen-Li Zhang Wei-Hua Jia Wei-Hua Jia Association Between Oral Microbiota and Cigarette Smoking in the Chinese Population Frontiers in Cellular and Infection Microbiology oral microbiota cigarette smoking 16S rRNA gene sequencing China saliva |
author_facet |
Yi-Jing Jia Yi-Jing Jia Ying Liao Yong-Qiao He Mei-Qi Zheng Xia-Ting Tong Xia-Ting Tong Wen-Qiong Xue Jiang-Bo Zhang Lei-Lei Yuan Lei-Lei Yuan Wen-Li Zhang Wei-Hua Jia Wei-Hua Jia |
author_sort |
Yi-Jing Jia |
title |
Association Between Oral Microbiota and Cigarette Smoking in the Chinese Population |
title_short |
Association Between Oral Microbiota and Cigarette Smoking in the Chinese Population |
title_full |
Association Between Oral Microbiota and Cigarette Smoking in the Chinese Population |
title_fullStr |
Association Between Oral Microbiota and Cigarette Smoking in the Chinese Population |
title_full_unstemmed |
Association Between Oral Microbiota and Cigarette Smoking in the Chinese Population |
title_sort |
association between oral microbiota and cigarette smoking in the chinese population |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2021-05-01 |
description |
The oral microbiota has been observed to be influenced by cigarette smoking and linked to several human diseases. However, research on the effect of cigarette smoking on the oral microbiota has not been systematically conducted in the Chinese population. We profiled the oral microbiota of 316 healthy subjects in the Chinese population by 16S rRNA gene sequencing. The alpha diversity of oral microbiota was different between never smokers and smokers (P = 0.002). Several bacterial taxa were first reported to be associated with cigarette smoking by LEfSe analysis, including Moryella (q = 1.56E-04), Bulleidia (q = 1.65E-06), and Moraxella (q = 3.52E-02) at the genus level and Rothia dentocariosa (q = 1.55E-02), Prevotella melaninogenica (q = 8.48E-08), Prevotella pallens (q = 4.13E-03), Bulleidia moorei (q = 1.79E-06), Rothia aeria (q = 3.83E-06), Actinobacillus parahaemolyticus (q = 2.28E-04), and Haemophilus parainfluenzae (q = 4.82E-02) at the species level. Two nitrite-producing bacteria that can increase the acidity of the oral cavity, Actinomyces and Veillonella, were also enriched in smokers with FDR-adjusted q-values of 3.62E-06 and 1.10E-06, respectively. Notably, we observed that two acid production-related pathways, amino acid-related enzymes (q = 6.19E-05) and amino sugar and nucleotide sugar metabolism (q = 2.63E-06), were increased in smokers by PICRUSt analysis. Finally, the co-occurrence analysis demonstrated that smoker-enriched bacteria were significantly positively associated with each other and were negatively correlated with the bacteria decreased in smokers. Our results suggested that cigarette smoking may affect oral health by creating a different environment by altering bacterial abundance, connections among oral microbiota, and the microbiota and their metabolic function. |
topic |
oral microbiota cigarette smoking 16S rRNA gene sequencing China saliva |
url |
https://www.frontiersin.org/articles/10.3389/fcimb.2021.658203/full |
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doaj-712783e35f3e4413a5c432a08cc751342021-05-28T14:46:04ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-05-011110.3389/fcimb.2021.658203658203Association Between Oral Microbiota and Cigarette Smoking in the Chinese PopulationYi-Jing Jia0Yi-Jing Jia1Ying Liao2Yong-Qiao He3Mei-Qi Zheng4Xia-Ting Tong5Xia-Ting Tong6Wen-Qiong Xue7Jiang-Bo Zhang8Lei-Lei Yuan9Lei-Lei Yuan10Wen-Li Zhang11Wei-Hua Jia12Wei-Hua Jia13School of Public Health, Sun Yat‐sen University, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat‐sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat‐sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat‐sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat‐sen University Cancer Center, Guangzhou, ChinaSchool of Public Health, Sun Yat‐sen University, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat‐sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat‐sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat‐sen University Cancer Center, Guangzhou, ChinaSchool of Public Health, Sun Yat‐sen University, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat‐sen University Cancer Center, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat‐sen University Cancer Center, Guangzhou, ChinaSchool of Public Health, Sun Yat‐sen University, Guangzhou, ChinaState Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Sun Yat‐sen University Cancer Center, Guangzhou, ChinaThe oral microbiota has been observed to be influenced by cigarette smoking and linked to several human diseases. However, research on the effect of cigarette smoking on the oral microbiota has not been systematically conducted in the Chinese population. We profiled the oral microbiota of 316 healthy subjects in the Chinese population by 16S rRNA gene sequencing. The alpha diversity of oral microbiota was different between never smokers and smokers (P = 0.002). Several bacterial taxa were first reported to be associated with cigarette smoking by LEfSe analysis, including Moryella (q = 1.56E-04), Bulleidia (q = 1.65E-06), and Moraxella (q = 3.52E-02) at the genus level and Rothia dentocariosa (q = 1.55E-02), Prevotella melaninogenica (q = 8.48E-08), Prevotella pallens (q = 4.13E-03), Bulleidia moorei (q = 1.79E-06), Rothia aeria (q = 3.83E-06), Actinobacillus parahaemolyticus (q = 2.28E-04), and Haemophilus parainfluenzae (q = 4.82E-02) at the species level. Two nitrite-producing bacteria that can increase the acidity of the oral cavity, Actinomyces and Veillonella, were also enriched in smokers with FDR-adjusted q-values of 3.62E-06 and 1.10E-06, respectively. Notably, we observed that two acid production-related pathways, amino acid-related enzymes (q = 6.19E-05) and amino sugar and nucleotide sugar metabolism (q = 2.63E-06), were increased in smokers by PICRUSt analysis. Finally, the co-occurrence analysis demonstrated that smoker-enriched bacteria were significantly positively associated with each other and were negatively correlated with the bacteria decreased in smokers. Our results suggested that cigarette smoking may affect oral health by creating a different environment by altering bacterial abundance, connections among oral microbiota, and the microbiota and their metabolic function.https://www.frontiersin.org/articles/10.3389/fcimb.2021.658203/fulloral microbiotacigarette smoking16S rRNA gene sequencingChinasaliva |