Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible Mechanisms
Transformation is one mode of horizontal gene transfer (HGT) in bacteria, wherein extracellular naked DNA is taken up by cells that have developed genetic competence. Sensitivity to DNase, which degrades naked DNA, is the key to distinguishing transformation from the DNase-resistant HGT mechanisms....
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2018-10-01
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doaj-7132e99652754081a21dccfb8ad0ffb42020-11-25T00:46:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-10-01910.3389/fmicb.2018.02365409418Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible MechanismsHaruka HasegawaErika SuzukiSumio MaedaTransformation is one mode of horizontal gene transfer (HGT) in bacteria, wherein extracellular naked DNA is taken up by cells that have developed genetic competence. Sensitivity to DNase, which degrades naked DNA, is the key to distinguishing transformation from the DNase-resistant HGT mechanisms. In general, Escherichia coli is not believed to be naturally transformable; it develops high competence only under artificial conditions, including exposure to high Ca2+ concentrations. However, E. coli can reportedly express modest competence under certain conditions that are feasible in natural environments outside laboratory. In addition, recent data suggest that environmental factors influence multiple routes of transformation. In this mini review, we (1) summarize our studies on transformation-based HGT using E. coli experimental systems and (2) discuss the possible occurrence of transformation via multiple mechanisms in the environment and its possible impact on the spread of antibiotic resistance genes.https://www.frontiersin.org/article/10.3389/fmicb.2018.02365/fullplasmid transformationhorizontal plasmid transferEscherichia coliantibiotic resistancesolid-air biofilm |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haruka Hasegawa Erika Suzuki Sumio Maeda |
spellingShingle |
Haruka Hasegawa Erika Suzuki Sumio Maeda Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible Mechanisms Frontiers in Microbiology plasmid transformation horizontal plasmid transfer Escherichia coli antibiotic resistance solid-air biofilm |
author_facet |
Haruka Hasegawa Erika Suzuki Sumio Maeda |
author_sort |
Haruka Hasegawa |
title |
Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible Mechanisms |
title_short |
Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible Mechanisms |
title_full |
Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible Mechanisms |
title_fullStr |
Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible Mechanisms |
title_full_unstemmed |
Horizontal Plasmid Transfer by Transformation in Escherichia coli: Environmental Factors and Possible Mechanisms |
title_sort |
horizontal plasmid transfer by transformation in escherichia coli: environmental factors and possible mechanisms |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-10-01 |
description |
Transformation is one mode of horizontal gene transfer (HGT) in bacteria, wherein extracellular naked DNA is taken up by cells that have developed genetic competence. Sensitivity to DNase, which degrades naked DNA, is the key to distinguishing transformation from the DNase-resistant HGT mechanisms. In general, Escherichia coli is not believed to be naturally transformable; it develops high competence only under artificial conditions, including exposure to high Ca2+ concentrations. However, E. coli can reportedly express modest competence under certain conditions that are feasible in natural environments outside laboratory. In addition, recent data suggest that environmental factors influence multiple routes of transformation. In this mini review, we (1) summarize our studies on transformation-based HGT using E. coli experimental systems and (2) discuss the possible occurrence of transformation via multiple mechanisms in the environment and its possible impact on the spread of antibiotic resistance genes. |
topic |
plasmid transformation horizontal plasmid transfer Escherichia coli antibiotic resistance solid-air biofilm |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.02365/full |
work_keys_str_mv |
AT harukahasegawa horizontalplasmidtransferbytransformationinescherichiacolienvironmentalfactorsandpossiblemechanisms AT erikasuzuki horizontalplasmidtransferbytransformationinescherichiacolienvironmentalfactorsandpossiblemechanisms AT sumiomaeda horizontalplasmidtransferbytransformationinescherichiacolienvironmentalfactorsandpossiblemechanisms |
_version_ |
1725264289544011776 |