Strain screening and optimization of biohydrogen production by Enterobacter aerogenes EB-06 from glycerol fermentation
Abstract Biohydrogen technology has drawn much attention due to its many advantages. However, it is still necessary to screen much more strains with stronger hydrogen-producing capacity for future commercialization processes. In this paper, a biohydrogen-producing strain Enterobacter aerogenes EB-06...
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doaj-713324016885414f828346deb3b9ac362020-11-25T02:54:24ZengSpringerOpenBioresources and Bioprocessing2197-43652019-05-016111310.1186/s40643-019-0250-zStrain screening and optimization of biohydrogen production by Enterobacter aerogenes EB-06 from glycerol fermentationYifeng Li0Yongqiu Qiu1Xu Zhang2Minglong Zhu3Wensong Tan4State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyAbstract Biohydrogen technology has drawn much attention due to its many advantages. However, it is still necessary to screen much more strains with stronger hydrogen-producing capacity for future commercialization processes. In this paper, a biohydrogen-producing strain Enterobacter aerogenes EB-06 was isolated, identified, and named. It could convert glycerol to biohydrogen by microorganism fermentation. The effects of oxygen content, initial pH, initial glycerol concentration, and initial nitrogen source content on biohydrogen production process were investigated. The results have shown that biohydrogen generation was more favorable under anaerobic conditions. The optimum specific biohydrogen production rate (Q H2) was obtained as 41.47 mmol H2/g DCW h at 40 g/L initial glycerol concentration. The optimum volume H2 yield (C H2) was 83.76 mmol H2/L at initial pH 7.0. It was found that nitrogen source content (0–4 g/L) could promote biohydrogen production and cell growth. The biohydrogen production of Enterobacter aerogenes EB-06 from glycerol was optimized by the orthogonal experimental design. The optimal yield coefficient of biohydrogen from glycerol fermentation (Y H2/glycerol) of EB-06 was obtained as 1.07 mmol H2/ mol glycerol at 10 g/L initial glycerol concentration, initial pH 5.0, and initial C/N ratio 5/3.http://link.springer.com/article/10.1186/s40643-019-0250-zBiohydrogen productionCondition optimizationStrain screeningEnterobacter aerogenesGlycerol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yifeng Li Yongqiu Qiu Xu Zhang Minglong Zhu Wensong Tan |
spellingShingle |
Yifeng Li Yongqiu Qiu Xu Zhang Minglong Zhu Wensong Tan Strain screening and optimization of biohydrogen production by Enterobacter aerogenes EB-06 from glycerol fermentation Bioresources and Bioprocessing Biohydrogen production Condition optimization Strain screening Enterobacter aerogenes Glycerol |
author_facet |
Yifeng Li Yongqiu Qiu Xu Zhang Minglong Zhu Wensong Tan |
author_sort |
Yifeng Li |
title |
Strain screening and optimization of biohydrogen production by Enterobacter aerogenes EB-06 from glycerol fermentation |
title_short |
Strain screening and optimization of biohydrogen production by Enterobacter aerogenes EB-06 from glycerol fermentation |
title_full |
Strain screening and optimization of biohydrogen production by Enterobacter aerogenes EB-06 from glycerol fermentation |
title_fullStr |
Strain screening and optimization of biohydrogen production by Enterobacter aerogenes EB-06 from glycerol fermentation |
title_full_unstemmed |
Strain screening and optimization of biohydrogen production by Enterobacter aerogenes EB-06 from glycerol fermentation |
title_sort |
strain screening and optimization of biohydrogen production by enterobacter aerogenes eb-06 from glycerol fermentation |
publisher |
SpringerOpen |
series |
Bioresources and Bioprocessing |
issn |
2197-4365 |
publishDate |
2019-05-01 |
description |
Abstract Biohydrogen technology has drawn much attention due to its many advantages. However, it is still necessary to screen much more strains with stronger hydrogen-producing capacity for future commercialization processes. In this paper, a biohydrogen-producing strain Enterobacter aerogenes EB-06 was isolated, identified, and named. It could convert glycerol to biohydrogen by microorganism fermentation. The effects of oxygen content, initial pH, initial glycerol concentration, and initial nitrogen source content on biohydrogen production process were investigated. The results have shown that biohydrogen generation was more favorable under anaerobic conditions. The optimum specific biohydrogen production rate (Q H2) was obtained as 41.47 mmol H2/g DCW h at 40 g/L initial glycerol concentration. The optimum volume H2 yield (C H2) was 83.76 mmol H2/L at initial pH 7.0. It was found that nitrogen source content (0–4 g/L) could promote biohydrogen production and cell growth. The biohydrogen production of Enterobacter aerogenes EB-06 from glycerol was optimized by the orthogonal experimental design. The optimal yield coefficient of biohydrogen from glycerol fermentation (Y H2/glycerol) of EB-06 was obtained as 1.07 mmol H2/ mol glycerol at 10 g/L initial glycerol concentration, initial pH 5.0, and initial C/N ratio 5/3. |
topic |
Biohydrogen production Condition optimization Strain screening Enterobacter aerogenes Glycerol |
url |
http://link.springer.com/article/10.1186/s40643-019-0250-z |
work_keys_str_mv |
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