COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies
Although molecular testing, and RT-qPCR in particular, has been an indispensable component in the scientific armoury targeting SARS-CoV-2, there are numerous falsehoods, misconceptions, assumptions and exaggerated expectations with regards to capability, performance and usefulness of the technology....
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doaj-7137d7800fb24d769b24752c029924202021-03-01T00:04:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-02-01222459245910.3390/ijms22052459COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and FallaciesStephen Bustin0Reinhold Mueller1Gregory Shipley2Tania Nolan3Medical Technology Research Centre, Anglia Ruskin University, Chelmsford CM1 1SQ, UKRM Consulting, San Diego, CA 92037, USAShipley Consulting, Vancouver, WA 98682, USAMedical Technology Research Centre, Anglia Ruskin University, Chelmsford CM1 1SQ, UKAlthough molecular testing, and RT-qPCR in particular, has been an indispensable component in the scientific armoury targeting SARS-CoV-2, there are numerous falsehoods, misconceptions, assumptions and exaggerated expectations with regards to capability, performance and usefulness of the technology. It is essential that the true strengths and limitations, although publicised for at least twenty years, are restated in the context of the current COVID-19 epidemic. The main objective of this commentary is to address and help stop the unfounded and debilitating speculation surrounding its use.https://www.mdpi.com/1422-0067/22/5/2459COVID-19SARS-CoV-2molecular diagnosticsquantification cycleRT-qPCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stephen Bustin Reinhold Mueller Gregory Shipley Tania Nolan |
spellingShingle |
Stephen Bustin Reinhold Mueller Gregory Shipley Tania Nolan COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies International Journal of Molecular Sciences COVID-19 SARS-CoV-2 molecular diagnostics quantification cycle RT-qPCR |
author_facet |
Stephen Bustin Reinhold Mueller Gregory Shipley Tania Nolan |
author_sort |
Stephen Bustin |
title |
COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies |
title_short |
COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies |
title_full |
COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies |
title_fullStr |
COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies |
title_full_unstemmed |
COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies |
title_sort |
covid-19 and diagnostic testing for sars-cov-2 by rt-qpcr—facts and fallacies |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-02-01 |
description |
Although molecular testing, and RT-qPCR in particular, has been an indispensable component in the scientific armoury targeting SARS-CoV-2, there are numerous falsehoods, misconceptions, assumptions and exaggerated expectations with regards to capability, performance and usefulness of the technology. It is essential that the true strengths and limitations, although publicised for at least twenty years, are restated in the context of the current COVID-19 epidemic. The main objective of this commentary is to address and help stop the unfounded and debilitating speculation surrounding its use. |
topic |
COVID-19 SARS-CoV-2 molecular diagnostics quantification cycle RT-qPCR |
url |
https://www.mdpi.com/1422-0067/22/5/2459 |
work_keys_str_mv |
AT stephenbustin covid19anddiagnostictestingforsarscov2byrtqpcrfactsandfallacies AT reinholdmueller covid19anddiagnostictestingforsarscov2byrtqpcrfactsandfallacies AT gregoryshipley covid19anddiagnostictestingforsarscov2byrtqpcrfactsandfallacies AT tanianolan covid19anddiagnostictestingforsarscov2byrtqpcrfactsandfallacies |
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1724247147398823936 |