Biocatalysis of Fucodian in <i>Undaria pinnatifida</i> Sporophyll Using <i>Bifidobacterium longum</i> RD47 for Production of Prebiotic Fucosylated Oligosaccharide

Fucosylated oligosaccharide (FO) is known to selectively promote the growth of probiotic bacteria and is currently marketed as a functional health food and prebiotic in infant formula. Despite widespread interest in FO among functional food customers, high production costs due to high raw material c...

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Main Authors: Jeong Min Lee, So Young Oh, Tony V. Johnston, Seockmo Ku, Geun Eog Ji
Format: Article
Language:English
Published: MDPI AG 2019-02-01
Series:Marine Drugs
Subjects:
Online Access:https://www.mdpi.com/1660-3397/17/2/117
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spelling doaj-7144eed595024098a2f62938a07816c02020-11-25T00:27:25ZengMDPI AGMarine Drugs1660-33972019-02-0117211710.3390/md17020117md17020117Biocatalysis of Fucodian in <i>Undaria pinnatifida</i> Sporophyll Using <i>Bifidobacterium longum</i> RD47 for Production of Prebiotic Fucosylated OligosaccharideJeong Min Lee0So Young Oh1Tony V. Johnston2Seockmo Ku3Geun Eog Ji4Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 08826, KoreaDepartment of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 08826, KoreaFermentation Science Program, School of Agriculture, College of Basic and Applied Sciences, Middle Tennessee State University, Murfreesboro, TN 37132, USAFermentation Science Program, School of Agriculture, College of Basic and Applied Sciences, Middle Tennessee State University, Murfreesboro, TN 37132, USADepartment of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 08826, KoreaFucosylated oligosaccharide (FO) is known to selectively promote the growth of probiotic bacteria and is currently marketed as a functional health food and prebiotic in infant formula. Despite widespread interest in FO among functional food customers, high production costs due to high raw material costs, especially those related to fucose, are a significant production issue. Therefore, several actions are required before efficient large-scale operations can occur, including (i) identification of inexpensive raw materials from which fucosylated oligosaccharides may be produced and (ii) development of production methods to which functional food consumers will not object (e.g., no genetically modified organisms (GMOs)). <i>Undaria pinnatifida</i>, commonly called Miyeok in Korea, is a common edible brown seaweed plentiful on the shores of the Korean peninsula. In particular, the sporophyll of <i>Undaria pinnatifida</i> contains significant levels of <span style="font-variant: small-caps;">l</span>-fucose in the form of fucoidan (a marine sulfated polysaccharide). If the <span style="font-variant: small-caps;">l</span>-fucose present in <i>Undaria pinnatifida</i> sporophyll was capable of being separated and recovered, <span style="font-variant: small-caps;">l</span>-fucose molecules could be covalently joined to other monosaccharides via glycosidic linkages, making this FO manufacturing technology of value in the functional food market. In our previous work, &#946;-galactosidase (EC 3.2.2.23) from <i>Bifidobacterium longum</i> RD47 (<i>B. longum</i> RD47) was found to have transglycosylation activity and produce FO using purified <span style="font-variant: small-caps;">l</span>-fucose and lactose as substrates. In this research, crude fucodian hydrolysates were separated and recovered from edible seaweed (i.e., <i>U. pinnatifida</i> sporophyll). The extracted <span style="font-variant: small-caps;">l</span>-fucose was purified via gel permeation and ion exchange chromatographies and the recovered <span style="font-variant: small-caps;">l</span>-fucose was used to synthesize FO. <i>B. longum</i> RD47 successfully transglycosilated and produced FO using <span style="font-variant: small-caps;">l</span>-fucose derived from <i>Undaria pinnatifida</i> and lactose as substrates. To the best of our knowledge, this is the first report of synthesized FO using <i>Bifidobacterium</i> spp.https://www.mdpi.com/1660-3397/17/2/117fucosylated oligosaccharidemarine polysaccharide<span style="font-variant: small-caps">l</span>-fucosefucoidan<i>Undaria pinnatifida</i>prebiotics<i>Bifidobacterium</i>
collection DOAJ
language English
format Article
sources DOAJ
author Jeong Min Lee
So Young Oh
Tony V. Johnston
Seockmo Ku
Geun Eog Ji
spellingShingle Jeong Min Lee
So Young Oh
Tony V. Johnston
Seockmo Ku
Geun Eog Ji
Biocatalysis of Fucodian in <i>Undaria pinnatifida</i> Sporophyll Using <i>Bifidobacterium longum</i> RD47 for Production of Prebiotic Fucosylated Oligosaccharide
Marine Drugs
fucosylated oligosaccharide
marine polysaccharide
<span style="font-variant: small-caps">l</span>-fucose
fucoidan
<i>Undaria pinnatifida</i>
prebiotics
<i>Bifidobacterium</i>
author_facet Jeong Min Lee
So Young Oh
Tony V. Johnston
Seockmo Ku
Geun Eog Ji
author_sort Jeong Min Lee
title Biocatalysis of Fucodian in <i>Undaria pinnatifida</i> Sporophyll Using <i>Bifidobacterium longum</i> RD47 for Production of Prebiotic Fucosylated Oligosaccharide
title_short Biocatalysis of Fucodian in <i>Undaria pinnatifida</i> Sporophyll Using <i>Bifidobacterium longum</i> RD47 for Production of Prebiotic Fucosylated Oligosaccharide
title_full Biocatalysis of Fucodian in <i>Undaria pinnatifida</i> Sporophyll Using <i>Bifidobacterium longum</i> RD47 for Production of Prebiotic Fucosylated Oligosaccharide
title_fullStr Biocatalysis of Fucodian in <i>Undaria pinnatifida</i> Sporophyll Using <i>Bifidobacterium longum</i> RD47 for Production of Prebiotic Fucosylated Oligosaccharide
title_full_unstemmed Biocatalysis of Fucodian in <i>Undaria pinnatifida</i> Sporophyll Using <i>Bifidobacterium longum</i> RD47 for Production of Prebiotic Fucosylated Oligosaccharide
title_sort biocatalysis of fucodian in <i>undaria pinnatifida</i> sporophyll using <i>bifidobacterium longum</i> rd47 for production of prebiotic fucosylated oligosaccharide
publisher MDPI AG
series Marine Drugs
issn 1660-3397
publishDate 2019-02-01
description Fucosylated oligosaccharide (FO) is known to selectively promote the growth of probiotic bacteria and is currently marketed as a functional health food and prebiotic in infant formula. Despite widespread interest in FO among functional food customers, high production costs due to high raw material costs, especially those related to fucose, are a significant production issue. Therefore, several actions are required before efficient large-scale operations can occur, including (i) identification of inexpensive raw materials from which fucosylated oligosaccharides may be produced and (ii) development of production methods to which functional food consumers will not object (e.g., no genetically modified organisms (GMOs)). <i>Undaria pinnatifida</i>, commonly called Miyeok in Korea, is a common edible brown seaweed plentiful on the shores of the Korean peninsula. In particular, the sporophyll of <i>Undaria pinnatifida</i> contains significant levels of <span style="font-variant: small-caps;">l</span>-fucose in the form of fucoidan (a marine sulfated polysaccharide). If the <span style="font-variant: small-caps;">l</span>-fucose present in <i>Undaria pinnatifida</i> sporophyll was capable of being separated and recovered, <span style="font-variant: small-caps;">l</span>-fucose molecules could be covalently joined to other monosaccharides via glycosidic linkages, making this FO manufacturing technology of value in the functional food market. In our previous work, &#946;-galactosidase (EC 3.2.2.23) from <i>Bifidobacterium longum</i> RD47 (<i>B. longum</i> RD47) was found to have transglycosylation activity and produce FO using purified <span style="font-variant: small-caps;">l</span>-fucose and lactose as substrates. In this research, crude fucodian hydrolysates were separated and recovered from edible seaweed (i.e., <i>U. pinnatifida</i> sporophyll). The extracted <span style="font-variant: small-caps;">l</span>-fucose was purified via gel permeation and ion exchange chromatographies and the recovered <span style="font-variant: small-caps;">l</span>-fucose was used to synthesize FO. <i>B. longum</i> RD47 successfully transglycosilated and produced FO using <span style="font-variant: small-caps;">l</span>-fucose derived from <i>Undaria pinnatifida</i> and lactose as substrates. To the best of our knowledge, this is the first report of synthesized FO using <i>Bifidobacterium</i> spp.
topic fucosylated oligosaccharide
marine polysaccharide
<span style="font-variant: small-caps">l</span>-fucose
fucoidan
<i>Undaria pinnatifida</i>
prebiotics
<i>Bifidobacterium</i>
url https://www.mdpi.com/1660-3397/17/2/117
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