Studies on efficiency of RAPD primers in developing molecular profiles for genetic purity studies in soybean (Glycine max L.) cultivars
Major advances have recently been made in our understanding of soybean genetics and of the application of new technologies to soybean improvement. Estimates of genetic relationships on the basis of the enzymes and molecular markers have been shown to be consistent with expectations based on...
Main Author: | |
---|---|
Format: | Article |
Language: | English |
Published: |
Serbian Genetics Society
2014-01-01
|
Series: | Genetika |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0534-0012/2014/0534-00121403681K.pdf |
Summary: | Major advances have recently been made in our understanding of soybean
genetics and of the application of new technologies to soybean improvement.
Estimates of genetic relationships on the basis of the enzymes and molecular
markers have been shown to be consistent with expectations based on origin
and pedigree information. To identify efficient markers, that are to be used
for genetic purity studies, polymorphism is the basic criterion. RAPD has
been found to be an effective and efficient tool to evaluate and reveal
genetic polymorphism in several crop species. In present study a total of 80
RAPD primers were screened, out of which 37 gave amplification and only 30
primers showed unambiguous DNA profile. Out of these 30 primers, 22 gave
polymorphic banding patterns. It is evident from the result that, 30/80
primers tried (38%) provided unambiguous amplification and out of 30 primers,
22 primers (73%) were found to be polymorphic. Scorable 30 RAPD primers led
to amplification of 120 fragments out of which 81 (67.5%) bands were found to
be polymorphic. On an average, we got 4 bands per primer and 15 primers (50%)
have been found to produce more number of bands than the average value which
is encouraging. 8 primers were found to give 100% polymorphisms. Our results
are indicative of the efficiency of RAPD primers towards development of
molecular profiles. |
---|---|
ISSN: | 0534-0012 1820-6069 |