Assessment of the metabolism of different strains of Bacillus megaterium

Assessment of the metabolism of different strains of Bacillus megaterium

Bacillus megaterium is a promising host for expression of heterologous proteins. This paper reports the nutrient consumption patterns and production of metabolites for three different strains of B. megaterium, ATCC 14945, QMB 1551 and PV 361, which is QMB 1551 with seven constitutive plasmids delete...

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Bibliographic Details
Main Authors: Carlos Alberto Galeano Suarez, Inti Doraci Cavalcanti Montano, Edson Romano Nucci, Monica Rosas da Costa Iemma, Raquel de Lima Camargo Giordano, Roberto de Campos Giordano
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2012-08-01
Series:Brazilian Archives of Biology and Technology
Subjects:
metabolic profile
Bacillus megaterium
culture media
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400001
Description
Summary:Bacillus megaterium is a promising host for expression of heterologous proteins. This paper reports the nutrient consumption patterns and production of metabolites for three different strains of B. megaterium, ATCC 14945, QMB 1551 and PV 361, which is QMB 1551 with seven constitutive plasmids deleted. 14 h cultivations in agitated flasks were run, for two different media: A (LB plus 10g/L glucose) and B (medium A, with the yeast extract replaced by tryptone). Strains PV361 and QMB 1551 showed higher maximum specific growth rates in medium B, reaching 0.42 h-1 and 0.48 h-1 respectively. The main by-products of the glucose overflow mechanism were acetate and lactate, for all three strains, which had preferential amino acids for substrate: Ala, Asp, Glu, Ser. No production of alcohols was detected.
ISSN:1516-8913
1678-4324

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