Protective Effects of Quercetin on Hepatic Ischemia-Reperfusion Injury
Introduction: The present study was designed to evaluate the effects of quercetin on hepatic ischemia-reperfusion (IR) injury in rats caused by Pringle maneuver.Methods: Overall, 24 5- to 6-month-old female Wistar Albino rats weighing 200-250 mg were included in the present study. In the sham group,...
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doaj-7164a6b0f0e847c89d5707dcf687ad882020-11-24T22:06:23ZengGalenos Yayineviİstanbul Medical Journal2619-97932148-094X2018-03-01191475110.5152/imj.2018.7232513049054Protective Effects of Quercetin on Hepatic Ischemia-Reperfusion InjuryHalil Mert Bahadir0Talha Sarigoz1Ömer Topuz2Yusuf Sevim3Tamer Ertan4İnanç Şamil Sarıcı5 Clinic of General Surgery, Erzin State Hospital, Hatay, Türkiye Clinic of General Surgery, Sason State Hospital, Batman, Türkiye Clinic of General Surgery, Kayseri Training and Research Hospital, Kayseri, Türkiye Clinic of General Surgery, Numune Training and Research Hospital, Ankara, Türkiye Clinic of General Surgery, Kayseri Training and Research Hospital, Kayseri, Türkiye Clinic of General Surgery, Kanuni Sultan Süleyman Training and Research Hospital, İstanbul, Türkiye Introduction: The present study was designed to evaluate the effects of quercetin on hepatic ischemia-reperfusion (IR) injury in rats caused by Pringle maneuver.Methods: Overall, 24 5- to 6-month-old female Wistar Albino rats weighing 200-250 mg were included in the present study. In the sham group, laparotomy was performed 15 min after anesthesia induction. Ischemia was not created. In the other groups, the hepatic pedicle (portal vein, hepatic artery, and bile duct) was explored, and ischemia was created with an atraumatic microvascular clamp. Concurrently, the exposed abdomen was covered with warm gauze soaked in saline. In the study group, quercetin was intraperitoneally injected prior to laparotomy and IR. Liver tissue samples from the left lobe were analyzed under a light microscope for liver damage. For the evaluation of hepatic IR injury and assessment of the effect of quercetin on antioxidant systems, total oxidant status (TOS), and total antioxidant status (TAS) levels were measured. Oxidative stress index (OSI) was calculated.Results: Aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase values of the control group were significantly higher than those of the sham and the study groups (p<0.001). Although the mean TAS, TOS, and OSI values of the study group were found to be lower than those of the control group, the difference was not statistically significant (p>0.05). Significantly less vacuolization and sinusoidal dilatation were observed in the study group than in the control group (p<0.05). For necrosis and apoptosis, there was no significant difference between the control and the study groups (p>0.05).Conclusion: On consideration of all findings, quercetin, which hosts many molecules within its structure with a potential utility, such as an antiinflammatory agent, antioxidant, and antiaggregant, has an effect that may be protective against hepatic IR injury. http://imj.galenos.com.tr/archives/archive-detail/article-preview/protective-effects-of-quercetin-on-hepatic-schemia/20351 Quercetinischemia-reperfusionliver injuryPringle maneuverrat modelantioxidant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Halil Mert Bahadir Talha Sarigoz Ömer Topuz Yusuf Sevim Tamer Ertan İnanç Şamil Sarıcı |
spellingShingle |
Halil Mert Bahadir Talha Sarigoz Ömer Topuz Yusuf Sevim Tamer Ertan İnanç Şamil Sarıcı Protective Effects of Quercetin on Hepatic Ischemia-Reperfusion Injury İstanbul Medical Journal Quercetin ischemia-reperfusion liver injury Pringle maneuver rat model antioxidant |
author_facet |
Halil Mert Bahadir Talha Sarigoz Ömer Topuz Yusuf Sevim Tamer Ertan İnanç Şamil Sarıcı |
author_sort |
Halil Mert Bahadir |
title |
Protective Effects of Quercetin on Hepatic Ischemia-Reperfusion Injury |
title_short |
Protective Effects of Quercetin on Hepatic Ischemia-Reperfusion Injury |
title_full |
Protective Effects of Quercetin on Hepatic Ischemia-Reperfusion Injury |
title_fullStr |
Protective Effects of Quercetin on Hepatic Ischemia-Reperfusion Injury |
title_full_unstemmed |
Protective Effects of Quercetin on Hepatic Ischemia-Reperfusion Injury |
title_sort |
protective effects of quercetin on hepatic ischemia-reperfusion injury |
publisher |
Galenos Yayinevi |
series |
İstanbul Medical Journal |
issn |
2619-9793 2148-094X |
publishDate |
2018-03-01 |
description |
Introduction: The present study was designed to evaluate the effects of quercetin on hepatic ischemia-reperfusion (IR) injury in rats caused by Pringle maneuver.Methods: Overall, 24 5- to 6-month-old female Wistar Albino rats weighing 200-250 mg were included in the present study. In the sham group, laparotomy was performed 15 min after anesthesia induction. Ischemia was not created. In the other groups, the hepatic pedicle (portal vein, hepatic artery, and bile duct) was explored, and ischemia was created with an atraumatic microvascular clamp. Concurrently, the exposed abdomen was covered with warm gauze soaked in saline. In the study group, quercetin was intraperitoneally injected prior to laparotomy and IR. Liver tissue samples from the left lobe were analyzed under a light microscope for liver damage. For the evaluation of hepatic IR injury and assessment of the effect of quercetin on antioxidant systems, total oxidant status (TOS), and total antioxidant status (TAS) levels were measured. Oxidative stress index (OSI) was calculated.Results: Aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase values of the control group were significantly higher than those of the sham and the study groups (p<0.001). Although the mean TAS, TOS, and OSI values of the study group were found to be lower than those of the control group, the difference was not statistically significant (p>0.05). Significantly less vacuolization and sinusoidal dilatation were observed in the study group than in the control group (p<0.05). For necrosis and apoptosis, there was no significant difference between the control and the study groups (p>0.05).Conclusion: On consideration of all findings, quercetin, which hosts many molecules within its structure with a potential utility, such as an antiinflammatory agent, antioxidant, and antiaggregant, has an effect that may be protective against hepatic IR injury. |
topic |
Quercetin ischemia-reperfusion liver injury Pringle maneuver rat model antioxidant |
url |
http://imj.galenos.com.tr/archives/archive-detail/article-preview/protective-effects-of-quercetin-on-hepatic-schemia/20351
|
work_keys_str_mv |
AT halilmertbahadir protectiveeffectsofquercetinonhepaticischemiareperfusioninjury AT talhasarigoz protectiveeffectsofquercetinonhepaticischemiareperfusioninjury AT omertopuz protectiveeffectsofquercetinonhepaticischemiareperfusioninjury AT yusufsevim protectiveeffectsofquercetinonhepaticischemiareperfusioninjury AT tamerertan protectiveeffectsofquercetinonhepaticischemiareperfusioninjury AT inancsamilsarıcı protectiveeffectsofquercetinonhepaticischemiareperfusioninjury |
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1725823989370060800 |