Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes
Aquaglyceroporins—aquaporin membrane channels (AQP) that conduct glycerol and other small neutral solutes in addition to water—play major roles in obesity. In adipocytes, aquaglyceroporins mediate glycerol uptake and release across the plasma membrane, which are two key steps for triacylglycerols (T...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2017-11-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/18/12/2566 |
id |
doaj-717135fd18c34609a5724e4460c789a8 |
---|---|
record_format |
Article |
spelling |
doaj-717135fd18c34609a5724e4460c789a82020-11-24T21:04:31ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-11-011812256610.3390/ijms18122566ijms18122566Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 AdipocytesJeanne Durendale Chiadak0Patrizia Gena1Françoise Gregoire2Nargis Bolaky3Valérie Delforge4Jason Perret5Giuseppe Calamita6Christine Delporte7Laboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, 1070 Brussels, BelgiumDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “<i>Aldo Moro</i>”, 70125 Bari, ItalyLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, 1070 Brussels, BelgiumLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, 1070 Brussels, BelgiumLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, 1070 Brussels, BelgiumLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, 1070 Brussels, BelgiumDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari “<i>Aldo Moro</i>”, 70125 Bari, ItalyLaboratory of Pathophysiological and Nutritional Biochemistry, Université Libre de Bruxelles, 1070 Brussels, BelgiumAquaglyceroporins—aquaporin membrane channels (AQP) that conduct glycerol and other small neutral solutes in addition to water—play major roles in obesity. In adipocytes, aquaglyceroporins mediate glycerol uptake and release across the plasma membrane, which are two key steps for triacylglycerols (TAGs) synthesis (lipogenesis) and hydrolysis (lipolysis). The aim of this study was to assess both glycerol permeability and metabolism in undifferentiated 3T3-L1 cells (UDCs) as well as in untreated (CTL-DCs) versus lipopolysaccharide (LPS-DCs)-treated differentiated 3T3-L1 adipocytes. Glycerol release, TAGs content and whole membrane glycerol permeability were significantly increased in DCs as compared to UDCs. Moreover, in DCs, LPS treatment significantly increased TAGs content and decreased glycerol permeability. In addition, a significant reduction in whole membrane glycerol permeability was observed in LPS-DCs as compared to CTL-DCs. The relative contributions of AQP3, AQP7 and AQP9 (facilitated diffusion), as well as that of the phospholipid bilayer (simple diffusion), to the whole membrane glycerol permeability, were estimated biophysically in UDCs, CTL-DCs and LPS-DCs, using selective AQP inhibitors. Further studies will be required to determine if modifications in either subcellular localization and/or activity of aquaglyceroporins could account for the data herein. Nevertheless, our findings provide novel insights in understanding the LPS-induced adipocyte hypertrophy that accompanies obesity.https://www.mdpi.com/1422-0067/18/12/2566obesityglyceroltriacylglycerolsaquaglyceroporinsmembrane permeabilityadipocytes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeanne Durendale Chiadak Patrizia Gena Françoise Gregoire Nargis Bolaky Valérie Delforge Jason Perret Giuseppe Calamita Christine Delporte |
spellingShingle |
Jeanne Durendale Chiadak Patrizia Gena Françoise Gregoire Nargis Bolaky Valérie Delforge Jason Perret Giuseppe Calamita Christine Delporte Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes International Journal of Molecular Sciences obesity glycerol triacylglycerols aquaglyceroporins membrane permeability adipocytes |
author_facet |
Jeanne Durendale Chiadak Patrizia Gena Françoise Gregoire Nargis Bolaky Valérie Delforge Jason Perret Giuseppe Calamita Christine Delporte |
author_sort |
Jeanne Durendale Chiadak |
title |
Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes |
title_short |
Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes |
title_full |
Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes |
title_fullStr |
Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes |
title_full_unstemmed |
Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes |
title_sort |
lipopolysaccharide modifies glycerol permeability and metabolism in 3t3-l1 adipocytes |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2017-11-01 |
description |
Aquaglyceroporins—aquaporin membrane channels (AQP) that conduct glycerol and other small neutral solutes in addition to water—play major roles in obesity. In adipocytes, aquaglyceroporins mediate glycerol uptake and release across the plasma membrane, which are two key steps for triacylglycerols (TAGs) synthesis (lipogenesis) and hydrolysis (lipolysis). The aim of this study was to assess both glycerol permeability and metabolism in undifferentiated 3T3-L1 cells (UDCs) as well as in untreated (CTL-DCs) versus lipopolysaccharide (LPS-DCs)-treated differentiated 3T3-L1 adipocytes. Glycerol release, TAGs content and whole membrane glycerol permeability were significantly increased in DCs as compared to UDCs. Moreover, in DCs, LPS treatment significantly increased TAGs content and decreased glycerol permeability. In addition, a significant reduction in whole membrane glycerol permeability was observed in LPS-DCs as compared to CTL-DCs. The relative contributions of AQP3, AQP7 and AQP9 (facilitated diffusion), as well as that of the phospholipid bilayer (simple diffusion), to the whole membrane glycerol permeability, were estimated biophysically in UDCs, CTL-DCs and LPS-DCs, using selective AQP inhibitors. Further studies will be required to determine if modifications in either subcellular localization and/or activity of aquaglyceroporins could account for the data herein. Nevertheless, our findings provide novel insights in understanding the LPS-induced adipocyte hypertrophy that accompanies obesity. |
topic |
obesity glycerol triacylglycerols aquaglyceroporins membrane permeability adipocytes |
url |
https://www.mdpi.com/1422-0067/18/12/2566 |
work_keys_str_mv |
AT jeannedurendalechiadak lipopolysaccharidemodifiesglycerolpermeabilityandmetabolismin3t3l1adipocytes AT patriziagena lipopolysaccharidemodifiesglycerolpermeabilityandmetabolismin3t3l1adipocytes AT francoisegregoire lipopolysaccharidemodifiesglycerolpermeabilityandmetabolismin3t3l1adipocytes AT nargisbolaky lipopolysaccharidemodifiesglycerolpermeabilityandmetabolismin3t3l1adipocytes AT valeriedelforge lipopolysaccharidemodifiesglycerolpermeabilityandmetabolismin3t3l1adipocytes AT jasonperret lipopolysaccharidemodifiesglycerolpermeabilityandmetabolismin3t3l1adipocytes AT giuseppecalamita lipopolysaccharidemodifiesglycerolpermeabilityandmetabolismin3t3l1adipocytes AT christinedelporte lipopolysaccharidemodifiesglycerolpermeabilityandmetabolismin3t3l1adipocytes |
_version_ |
1716770789678645248 |