DIRECT EVALUATION OF OXIDATIVE STRESS AND ANTIOXIDANT DEFENSE PARAMETERS IN EXPERIMENT WITH THE AXIAL PUMP

• Main Authors: N. V. Kuncevich, G. P. Itkin, E. G. Konysheva, L. V. Makarova, O. P. Shevchenko
• Format: Article
• Language: Russian
• Published: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov 2011-12-01
• Series: Vestnik Transplantologii i Iskusstvennyh Organov
• Subjects: oxidative stress; axial pump; calves.
• Online Access: https://journal.transpl.ru/vtio/article/view/397

It is established that increased parameters of oxidative stress result from an imbalance between reactive oxygen molecules and endogenous antioxidants. Oxidative stress parameters may be considered to be biomarkers of heart failure. The aim of our study was to asses oxidative stress and antioxidant system parameters in experi- mental model with the axial pump. Our study included 7 calves (the mean age 120.33 days, the mean weight 99.17 kg). We used «FORM Plus CR3000» analyzer (Callegary, Italy) and heparinized venous blood samples for the evaluation of the oxidative status (FORT test) and the total antioxidant capacity (FORD test). In healthy animals oxidative system parameters differ from those in humans (FORT – 0.42 ± 0.11 versus 1.74 ± 0.32 mmol/l H2O2; FORD/FORT – 3.92 ± 1.30 versus 1.33 ± 0.27, p < 0.05) what should be taken into consideration when interpreting the results of clinical and experimental studies. In three calves with experimental assisted circulation maximal oxidative stress values were observed on 1–3 days after the axial pump connection with the subsequent normalization of these parameters. Direct evaluation of oxidative stress parameters may be used as monitoring tool for estimation of assisted circulation adequacy in experiment (animal model).