Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.

Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.

Listeria monocytogenes is a Gram-positive foodborne pathogen and the causative agent of listeriosis. L. monocytogenes lapB gene encodes a cell wall surface anchor protein, and mutation of this gene causes Listeria attenuation in mice. In this work, the potential role of Listeria LapB protein in catf...

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Main Authors: Ali Akgul, Nawar Al-Janabi, Bhaskar Das, Mark Lawrence, Attila Karsi
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5734760?pdf=render
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spelling doaj-7188d76e261f4c6da4a402acf7d62e5f2020-11-25T02:48:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011212e018980910.1371/journal.pone.0189809Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.Ali AkgulNawar Al-JanabiBhaskar DasMark LawrenceAttila KarsiListeria monocytogenes is a Gram-positive foodborne pathogen and the causative agent of listeriosis. L. monocytogenes lapB gene encodes a cell wall surface anchor protein, and mutation of this gene causes Listeria attenuation in mice. In this work, the potential role of Listeria LapB protein in catfish fillet attachment was investigated. To achieve this, boron-based small molecules designed to interfere with the active site of the L. monocytogenes LapB protein were developed, and their ability to prevent L. monocytogenes attachment to fish fillet was tested. Results indicated that seven out of nine different small molecules were effective in reducing the Listeria attachment to catfish fillets. Of these, three small molecules (SM3, SM5, and SM7) were highly effective in blocking Listeria attachment to catfish fillets. This study suggests an alternative strategy for reduction of L. monocytogenes contamination in fresh and frozen fish products.http://europepmc.org/articles/PMC5734760?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Ali Akgul
Nawar Al-Janabi
Bhaskar Das
Mark Lawrence
Attila Karsi
spellingShingle Ali Akgul
Nawar Al-Janabi
Bhaskar Das
Mark Lawrence
Attila Karsi
Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.
PLoS ONE
author_facet Ali Akgul
Nawar Al-Janabi
Bhaskar Das
Mark Lawrence
Attila Karsi
author_sort Ali Akgul
title Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.
title_short Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.
title_full Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.
title_fullStr Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.
title_full_unstemmed Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.
title_sort small molecules targeting lapb protein prevent listeria attachment to catfish muscle.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2017-01-01
description Listeria monocytogenes is a Gram-positive foodborne pathogen and the causative agent of listeriosis. L. monocytogenes lapB gene encodes a cell wall surface anchor protein, and mutation of this gene causes Listeria attenuation in mice. In this work, the potential role of Listeria LapB protein in catfish fillet attachment was investigated. To achieve this, boron-based small molecules designed to interfere with the active site of the L. monocytogenes LapB protein were developed, and their ability to prevent L. monocytogenes attachment to fish fillet was tested. Results indicated that seven out of nine different small molecules were effective in reducing the Listeria attachment to catfish fillets. Of these, three small molecules (SM3, SM5, and SM7) were highly effective in blocking Listeria attachment to catfish fillets. This study suggests an alternative strategy for reduction of L. monocytogenes contamination in fresh and frozen fish products.
url http://europepmc.org/articles/PMC5734760?pdf=render
work_keys_str_mv AT aliakgul smallmoleculestargetinglapbproteinpreventlisteriaattachmenttocatfishmuscle
AT nawaraljanabi smallmoleculestargetinglapbproteinpreventlisteriaattachmenttocatfishmuscle
AT bhaskardas smallmoleculestargetinglapbproteinpreventlisteriaattachmenttocatfishmuscle
AT marklawrence smallmoleculestargetinglapbproteinpreventlisteriaattachmenttocatfishmuscle
AT attilakarsi smallmoleculestargetinglapbproteinpreventlisteriaattachmenttocatfishmuscle
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