Surface charge-dependent mitochondrial response to similar intracellular nanoparticle contents at sublethal dosages

• Main Authors: Xiaoting Jin, Haiyi Yu, Ze Zhang, Tenglong Cui, Qi Wu, Xiaolei Liu, Jie Gao, Xingchen Zhao, Jianbo Shi, Guangbo Qu, Guibin Jiang
• Format: Article
• Language: English
• Published: BMC 2021-09-01
• Series: Particle and Fibre Toxicology
• Subjects: Nanoparticle; Mitochondrial response; Surface property; Sublethal dosage; Intracellular content
• Online Access: https://doi.org/10.1186/s12989-021-00429-8

Abstract Background Considering the inevitability for humans to be frequently exposed to nanoparticles (NPs), understanding the biosafety of NPs is important for rational usage. As an important part of the innate immune system, macrophages are widely distributed in vital tissues and are also a dominant cell type that engulfs particles. Mitochondria are one of the most sensitive organelles when macrophages are exposed to NPs. However, previous studies have mainly reported the mitochondrial response upon high-dose NP treatment. Herein, with gold nanoparticles (AuNPs) as a model, we investigated the mitochondrial alterations induced by NPs at a sublethal concentration. Results At a similar internal exposure dose, different AuNPs showed distinct degrees of effects on mitochondrial alterations, including reduced tubular mitochondria, damaged mitochondria, increased reactive oxygen species, and decreased adenosine triphosphate. Cluster analysis, two-way ANOVA, and multiple linear regression suggested that the surface properties of AuNPs were the dominant determinants of the mitochondrial response. Based on the correlation analysis, the mitochondrial response was increased with the change in zeta potential from negative to positive. The alterations in mitochondrial respiratory chain proteins indicated that complex V was an indicator of the mitochondrial response to low-dose NPs. Conclusion Our current study suggests potential hazards of modified AuNPs on mitochondria even under sublethal dose, indicates the possibility of surface modification in biocompatibility improvement, and provides a new way to better evaluation of nanomaterials biosafety.