2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.

• Main Authors: Ivanilda dos Santos Alves, Valéria Cristina Barbosa Carmazini, Cosme Damião dos Santos, Julieta Andrea Silva de Almeida
• Format: Article
• Language: English
• Published: Universidade Federal de Santa Maria 2018-11-01
• Series: Ciência Rural
• Subjects: Cultivar Mundo Novo; 2-iP; somatic embryos; embryogenic structures
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100403&lng=en&tlng=en

ABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively.