2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.
ABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and...
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Universidade Federal de Santa Maria
2018-11-01
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doaj-718fcfd7cc2c4fbd81c454c8f8bd4d7d2020-11-24T21:12:42ZengUniversidade Federal de Santa MariaCiência Rural1678-45962018-11-01481110.1590/0103-8478cr20180001S0103-847820180011004032- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L.Ivanilda dos Santos AlvesValéria Cristina Barbosa CarmaziniCosme Damião dos SantosJulieta Andrea Silva de AlmeidaABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100403&lng=en&tlng=enCultivar Mundo Novo2-iPsomatic embryosembryogenic structures |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ivanilda dos Santos Alves Valéria Cristina Barbosa Carmazini Cosme Damião dos Santos Julieta Andrea Silva de Almeida |
spellingShingle |
Ivanilda dos Santos Alves Valéria Cristina Barbosa Carmazini Cosme Damião dos Santos Julieta Andrea Silva de Almeida 2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. Ciência Rural Cultivar Mundo Novo 2-iP somatic embryos embryogenic structures |
author_facet |
Ivanilda dos Santos Alves Valéria Cristina Barbosa Carmazini Cosme Damião dos Santos Julieta Andrea Silva de Almeida |
author_sort |
Ivanilda dos Santos Alves |
title |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
title_short |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
title_full |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
title_fullStr |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
title_full_unstemmed |
2- Isopentenyladenine in the induction of direct somatic embryogenesis capacity of Coffea arabica L. |
title_sort |
2- isopentenyladenine in the induction of direct somatic embryogenesis capacity of coffea arabica l. |
publisher |
Universidade Federal de Santa Maria |
series |
Ciência Rural |
issn |
1678-4596 |
publishDate |
2018-11-01 |
description |
ABSTRACT: This study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively. |
topic |
Cultivar Mundo Novo 2-iP somatic embryos embryogenic structures |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100403&lng=en&tlng=en |
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