Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic Cells
Chamomile, parsley, and celery represent major botanical sources of apigenin, a well-known flavone with chemopreventive properties. The aim of this study was to assess the phytochemical composition, antioxidant, and anti-inflammatory potential of methanol extracts obtained from chamomile, parsley, a...
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doaj-71a2ac0f8b96469084dea5a3e0883f6b2020-11-24T20:57:44ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-11-011911362410.3390/ijms19113624ijms19113624Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic CellsCorina Danciu0Istvan Zupko1Andrea Bor2Anja Schwiebs3Heinfried Radeke4Monica Hancianu5Oana Cioanca6Ersilia Alexa7Camelia Oprean8Florina Bojin9Codruta Soica10Virgil Paunescu11Cristina Adriana Dehelean12Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy, 300041 Timişoara, RomaniaFaculty of Pharmacy, University of Szeged, H-6720 Szeged, HungaryFaculty of Pharmacy, University of Szeged, H-6720 Szeged, HungaryPharmazentrum frankfurt/ZAFES, Clinic of the Goethe University, D-60590 Frankfurt/Main, GermanyPharmazentrum frankfurt/ZAFES, Clinic of the Goethe University, D-60590 Frankfurt/Main, GermanyFaculty of Pharmacy, “Gr.T.Popa” University of Medicine and Pharmacy, 700115 Iasi, RomaniaFaculty of Pharmacy, “Gr.T.Popa” University of Medicine and Pharmacy, 700115 Iasi, RomaniaBanat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, 300645 Timisoara, RomaniaFaculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy, 300041 Timişoara, RomaniaFaculty of Medicine, “Victor Babeş” University of Medicine and Pharmacy, 300041 Timişoara, RomaniaFaculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy, 300041 Timişoara, RomaniaFaculty of Medicine, “Victor Babeş” University of Medicine and Pharmacy, 300041 Timişoara, RomaniaFaculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy, 300041 Timişoara, RomaniaChamomile, parsley, and celery represent major botanical sources of apigenin, a well-known flavone with chemopreventive properties. The aim of this study was to assess the phytochemical composition, antioxidant, and anti-inflammatory potential of methanol extracts obtained from chamomile, parsley, and celery collected from Romania, as well as the biological activity against A375 human melanoma and human dendritic cells. Results have shown that all three extracts are rich in polyphenolic compounds and flavonoids, and they generate a radical scavenger capacity, iron chelation potential, as well as lipoxygenase inhibition capacity. Chamomile and celery extracts present weak antiproliferative and pro-apoptotic properties in the set experimental conditions, while parsley extract draws out significant pro-apoptotic potential against A375 human melanoma cells. Parsley and chamomile extracts affected the fibroblast-like morphology of the screened tumor cell line. On the other hand, chamomile and celery extracts abrogated the expansion of LPS-activated dendritic cells, while the metabolic activity was attenuated by stimulation with celery extract; chamomile and parsley extracts had no effect upon this parameter. Chamomile and parsley extracts incubation with naive dendritic cells did not trigger cytokine secretion (TNF-alpha, IL-6, IL-10), but celery extract stimulation significantly reduced the anti-inflammatory, cytokine IL-10.https://www.mdpi.com/1422-0067/19/11/3624chamomileparsleyceleryantioxidantanti-inflammatoryA375 human melanoma cellsdendritic cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Corina Danciu Istvan Zupko Andrea Bor Anja Schwiebs Heinfried Radeke Monica Hancianu Oana Cioanca Ersilia Alexa Camelia Oprean Florina Bojin Codruta Soica Virgil Paunescu Cristina Adriana Dehelean |
spellingShingle |
Corina Danciu Istvan Zupko Andrea Bor Anja Schwiebs Heinfried Radeke Monica Hancianu Oana Cioanca Ersilia Alexa Camelia Oprean Florina Bojin Codruta Soica Virgil Paunescu Cristina Adriana Dehelean Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic Cells International Journal of Molecular Sciences chamomile parsley celery antioxidant anti-inflammatory A375 human melanoma cells dendritic cells |
author_facet |
Corina Danciu Istvan Zupko Andrea Bor Anja Schwiebs Heinfried Radeke Monica Hancianu Oana Cioanca Ersilia Alexa Camelia Oprean Florina Bojin Codruta Soica Virgil Paunescu Cristina Adriana Dehelean |
author_sort |
Corina Danciu |
title |
Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic Cells |
title_short |
Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic Cells |
title_full |
Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic Cells |
title_fullStr |
Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic Cells |
title_full_unstemmed |
Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic Cells |
title_sort |
botanical therapeutics: phytochemical screening and biological assessment of chamomile, parsley and celery extracts against a375 human melanoma and dendritic cells |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2018-11-01 |
description |
Chamomile, parsley, and celery represent major botanical sources of apigenin, a well-known flavone with chemopreventive properties. The aim of this study was to assess the phytochemical composition, antioxidant, and anti-inflammatory potential of methanol extracts obtained from chamomile, parsley, and celery collected from Romania, as well as the biological activity against A375 human melanoma and human dendritic cells. Results have shown that all three extracts are rich in polyphenolic compounds and flavonoids, and they generate a radical scavenger capacity, iron chelation potential, as well as lipoxygenase inhibition capacity. Chamomile and celery extracts present weak antiproliferative and pro-apoptotic properties in the set experimental conditions, while parsley extract draws out significant pro-apoptotic potential against A375 human melanoma cells. Parsley and chamomile extracts affected the fibroblast-like morphology of the screened tumor cell line. On the other hand, chamomile and celery extracts abrogated the expansion of LPS-activated dendritic cells, while the metabolic activity was attenuated by stimulation with celery extract; chamomile and parsley extracts had no effect upon this parameter. Chamomile and parsley extracts incubation with naive dendritic cells did not trigger cytokine secretion (TNF-alpha, IL-6, IL-10), but celery extract stimulation significantly reduced the anti-inflammatory, cytokine IL-10. |
topic |
chamomile parsley celery antioxidant anti-inflammatory A375 human melanoma cells dendritic cells |
url |
https://www.mdpi.com/1422-0067/19/11/3624 |
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