Genotoxicity Induced by Foetal and Infant Exposure to Magnetic Fields and Modulation of Ionising Radiation Effects.
Few studies have investigated the toxicity and genotoxicity of extremely low frequency magnetic fields (ELF-MF) during prenatal and neonatal development. These phases of life are characterized by cell proliferation and differentiation, which might make them sensitive to environmental stressors. Alth...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4641635?pdf=render |
id |
doaj-71a80039fde240b8bd4af3f537675fb9 |
---|---|
record_format |
Article |
spelling |
doaj-71a80039fde240b8bd4af3f537675fb92020-11-25T01:52:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014225910.1371/journal.pone.0142259Genotoxicity Induced by Foetal and Infant Exposure to Magnetic Fields and Modulation of Ionising Radiation Effects.Ion UdroiuAntonio AntocciaCaterina TanzarellaLivio GiulianiFrancesca PacchierottiEugenia CordelliPatrizia EleuteriPaola VillaniAntonella SguraFew studies have investigated the toxicity and genotoxicity of extremely low frequency magnetic fields (ELF-MF) during prenatal and neonatal development. These phases of life are characterized by cell proliferation and differentiation, which might make them sensitive to environmental stressors. Although in vitro evidences suggest that ELF-MF may modify the effects of ionizing radiation, no research has been conducted so far in vivo on the genotoxic effects of ELF-MF combined with X-rays.Aim of this study was to investigate in somatic and germ cells the effects of chronic ELF-MF exposure from mid gestation until weaning, and any possible modulation produced by ELF-MF exposure on ionizing radiation-induced damage. Mice were exposed to 50 Hz, 65 μT magnetic field, 24 hours/day, for a total of 30 days, starting from 12 days post-conception. Another group was irradiated with 1 Gy X-rays immediately before ELF-MF exposure, other groups were only X-irradiated or sham-exposed. Micronucleus test on blood erythrocytes was performed at multiple times from 1 to 140 days after birth. Additionally, 42 days after birth, genotoxic and cytotoxic effects on male germ cells were assessed by comet assay and flow cytometric analysis.ELF-MF exposure had no teratogenic effect and did not affect survival, growth and development. The micronucleus test indicated that ELF-MF induced a slight genotoxic damage only after the maximum exposure time and that this effect faded away in the months following the end of exposure. ELF-MF had no effects on ionizing radiation (IR)-induced genotoxicity in erythrocytes. Differently, ELF-MF appeared to modulate the response of male germ cells to X-rays with an impact on proliferation/differentiation processes. These results point to the importance of tissue specificity and development on the impact of ELF-MF on the early stages of life and indicate the need of further research on the molecular mechanisms underlying ELF-MF biological effects.http://europepmc.org/articles/PMC4641635?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ion Udroiu Antonio Antoccia Caterina Tanzarella Livio Giuliani Francesca Pacchierotti Eugenia Cordelli Patrizia Eleuteri Paola Villani Antonella Sgura |
spellingShingle |
Ion Udroiu Antonio Antoccia Caterina Tanzarella Livio Giuliani Francesca Pacchierotti Eugenia Cordelli Patrizia Eleuteri Paola Villani Antonella Sgura Genotoxicity Induced by Foetal and Infant Exposure to Magnetic Fields and Modulation of Ionising Radiation Effects. PLoS ONE |
author_facet |
Ion Udroiu Antonio Antoccia Caterina Tanzarella Livio Giuliani Francesca Pacchierotti Eugenia Cordelli Patrizia Eleuteri Paola Villani Antonella Sgura |
author_sort |
Ion Udroiu |
title |
Genotoxicity Induced by Foetal and Infant Exposure to Magnetic Fields and Modulation of Ionising Radiation Effects. |
title_short |
Genotoxicity Induced by Foetal and Infant Exposure to Magnetic Fields and Modulation of Ionising Radiation Effects. |
title_full |
Genotoxicity Induced by Foetal and Infant Exposure to Magnetic Fields and Modulation of Ionising Radiation Effects. |
title_fullStr |
Genotoxicity Induced by Foetal and Infant Exposure to Magnetic Fields and Modulation of Ionising Radiation Effects. |
title_full_unstemmed |
Genotoxicity Induced by Foetal and Infant Exposure to Magnetic Fields and Modulation of Ionising Radiation Effects. |
title_sort |
genotoxicity induced by foetal and infant exposure to magnetic fields and modulation of ionising radiation effects. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Few studies have investigated the toxicity and genotoxicity of extremely low frequency magnetic fields (ELF-MF) during prenatal and neonatal development. These phases of life are characterized by cell proliferation and differentiation, which might make them sensitive to environmental stressors. Although in vitro evidences suggest that ELF-MF may modify the effects of ionizing radiation, no research has been conducted so far in vivo on the genotoxic effects of ELF-MF combined with X-rays.Aim of this study was to investigate in somatic and germ cells the effects of chronic ELF-MF exposure from mid gestation until weaning, and any possible modulation produced by ELF-MF exposure on ionizing radiation-induced damage. Mice were exposed to 50 Hz, 65 μT magnetic field, 24 hours/day, for a total of 30 days, starting from 12 days post-conception. Another group was irradiated with 1 Gy X-rays immediately before ELF-MF exposure, other groups were only X-irradiated or sham-exposed. Micronucleus test on blood erythrocytes was performed at multiple times from 1 to 140 days after birth. Additionally, 42 days after birth, genotoxic and cytotoxic effects on male germ cells were assessed by comet assay and flow cytometric analysis.ELF-MF exposure had no teratogenic effect and did not affect survival, growth and development. The micronucleus test indicated that ELF-MF induced a slight genotoxic damage only after the maximum exposure time and that this effect faded away in the months following the end of exposure. ELF-MF had no effects on ionizing radiation (IR)-induced genotoxicity in erythrocytes. Differently, ELF-MF appeared to modulate the response of male germ cells to X-rays with an impact on proliferation/differentiation processes. These results point to the importance of tissue specificity and development on the impact of ELF-MF on the early stages of life and indicate the need of further research on the molecular mechanisms underlying ELF-MF biological effects. |
url |
http://europepmc.org/articles/PMC4641635?pdf=render |
work_keys_str_mv |
AT ionudroiu genotoxicityinducedbyfoetalandinfantexposuretomagneticfieldsandmodulationofionisingradiationeffects AT antonioantoccia genotoxicityinducedbyfoetalandinfantexposuretomagneticfieldsandmodulationofionisingradiationeffects AT caterinatanzarella genotoxicityinducedbyfoetalandinfantexposuretomagneticfieldsandmodulationofionisingradiationeffects AT liviogiuliani genotoxicityinducedbyfoetalandinfantexposuretomagneticfieldsandmodulationofionisingradiationeffects AT francescapacchierotti genotoxicityinducedbyfoetalandinfantexposuretomagneticfieldsandmodulationofionisingradiationeffects AT eugeniacordelli genotoxicityinducedbyfoetalandinfantexposuretomagneticfieldsandmodulationofionisingradiationeffects AT patriziaeleuteri genotoxicityinducedbyfoetalandinfantexposuretomagneticfieldsandmodulationofionisingradiationeffects AT paolavillani genotoxicityinducedbyfoetalandinfantexposuretomagneticfieldsandmodulationofionisingradiationeffects AT antonellasgura genotoxicityinducedbyfoetalandinfantexposuretomagneticfieldsandmodulationofionisingradiationeffects |
_version_ |
1724994083000156160 |