Translational Medicine Study on Extraction and Determination of Urtica Polysaccharides and Their Anti-Oxidation Effect

• Main Authors: Zhi-tao JIANG, Shan-shan LI, Tong-fang WANG, Xiao-feng CHEN
• Format: Article
• Language: English
• Published: Third Party Medicine International Publishing Group Co. Limited 2016-03-01
• Series: Journal of International Translational Medicine
• Subjects: Urtica; Urtica polysaccharides; Extraction; Quantitative measurement; Translational medicine; Anti-oxidation
• Online Access: http://www.jitm.hk/EN/abstract/abstract195.shtml#

Objective: Translational medicine study on extraction and determination of urtica polysaccharides and their anti-oxidation effect. Methods: Water-soluble alcohol sedimentation technique was used to extract the urtica polysaccharides, and ultraviolet (UV) spectrophotometry was applied to determine their contents. The influence of polysaccharides on the activities of glutathione (GSH), catalase (CAT), malondialdéhyde (MDA) and superoxyde dismutase (SOD) in mice with H22-loaded tumors was observed.Results: The purity of urtica polysaccharides was 61.39% after extraction and purification. After administration of high-, moderate- and low-dose urtica polysaccharides, the activity of serum GSH, CAT and SOD was markedly higher while MDA content was prominently lower in mice with H22-loaded tumors than those in model group.Conclusion: Urtica polysaccharides can strengthen the anti-oxidation effect and inhibit the lipid peroxidation reaction in body.