Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers
Abstract Background This study was conducted to examine effects of nitrate on ruminal methane production, methanogen abundance, and composition. Six rumen-fistulated Limousin×Jinnan steers were fed diets supplemented with either 0% (0NR), 1% (1NR), or 2% (2NR) nitrate (dry matter basis) regimens in...
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doaj-71c12b9ef877431184f4ba629e474de92020-11-25T02:41:56ZengBMCBMC Microbiology1471-21802018-03-011811810.1186/s12866-018-1164-1Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steersLiping Zhao0Qingxiang Meng1Yan Li2Hao Wu3Yunlong Huo4Xinzhuang Zhang5Zhenming Zhou6State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural UniversityState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural UniversityAbstract Background This study was conducted to examine effects of nitrate on ruminal methane production, methanogen abundance, and composition. Six rumen-fistulated Limousin×Jinnan steers were fed diets supplemented with either 0% (0NR), 1% (1NR), or 2% (2NR) nitrate (dry matter basis) regimens in succession. Rumen fluid was taken after two-week adaptation for evaluation of in vitro methane production, methanogen abundance, and composition measurements. Results Results showed that nitrate significantly decreased in vitro ruminal methane production at 6 h, 12 h, and 24 h (P < 0.01; P < 0.01; P = 0.01). The 1NR and 2NR regimens numerically reduced the methanogen population by 4.47% and 25.82% respectively. However, there was no significant difference observed between treatments. The alpha and beta diversity of the methanogen community was not significantly changed by nitrate either. However, the relative abundance of the methanogen genera was greatly changed. Methanosphaera (PL = 0.0033) and Methanimicrococcus (PL = 0.0113) abundance increased linearly commensurate with increasing nitration levels, while Methanoplanus abundance was significantly decreased (PL = 0.0013). The population of Methanoculleus, the least frequently identified genus in this study, exhibited quadratic growth from 0% to 2% when nitrate was added (PQ = 0.0140). Conclusions Correlation analysis found that methane reduction was significantly related to Methanobrevibacter and Methanoplanus abundance, and negatively correlated with Methanosphaera and Methanimicrococcus abundance.http://link.springer.com/article/10.1186/s12866-018-1164-1NitrateMethaneMethanogen diversityHiseq sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liping Zhao Qingxiang Meng Yan Li Hao Wu Yunlong Huo Xinzhuang Zhang Zhenming Zhou |
spellingShingle |
Liping Zhao Qingxiang Meng Yan Li Hao Wu Yunlong Huo Xinzhuang Zhang Zhenming Zhou Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers BMC Microbiology Nitrate Methane Methanogen diversity Hiseq sequencing |
author_facet |
Liping Zhao Qingxiang Meng Yan Li Hao Wu Yunlong Huo Xinzhuang Zhang Zhenming Zhou |
author_sort |
Liping Zhao |
title |
Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers |
title_short |
Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers |
title_full |
Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers |
title_fullStr |
Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers |
title_full_unstemmed |
Nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers |
title_sort |
nitrate decreases ruminal methane production with slight changes to ruminal methanogen composition of nitrate-adapted steers |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2018-03-01 |
description |
Abstract Background This study was conducted to examine effects of nitrate on ruminal methane production, methanogen abundance, and composition. Six rumen-fistulated Limousin×Jinnan steers were fed diets supplemented with either 0% (0NR), 1% (1NR), or 2% (2NR) nitrate (dry matter basis) regimens in succession. Rumen fluid was taken after two-week adaptation for evaluation of in vitro methane production, methanogen abundance, and composition measurements. Results Results showed that nitrate significantly decreased in vitro ruminal methane production at 6 h, 12 h, and 24 h (P < 0.01; P < 0.01; P = 0.01). The 1NR and 2NR regimens numerically reduced the methanogen population by 4.47% and 25.82% respectively. However, there was no significant difference observed between treatments. The alpha and beta diversity of the methanogen community was not significantly changed by nitrate either. However, the relative abundance of the methanogen genera was greatly changed. Methanosphaera (PL = 0.0033) and Methanimicrococcus (PL = 0.0113) abundance increased linearly commensurate with increasing nitration levels, while Methanoplanus abundance was significantly decreased (PL = 0.0013). The population of Methanoculleus, the least frequently identified genus in this study, exhibited quadratic growth from 0% to 2% when nitrate was added (PQ = 0.0140). Conclusions Correlation analysis found that methane reduction was significantly related to Methanobrevibacter and Methanoplanus abundance, and negatively correlated with Methanosphaera and Methanimicrococcus abundance. |
topic |
Nitrate Methane Methanogen diversity Hiseq sequencing |
url |
http://link.springer.com/article/10.1186/s12866-018-1164-1 |
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