Use of application containers and workflows for genomic data analysis

• Main Authors: Wade L Schulz, Thomas Durant, Alexa J Siddon, Richard Torres
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2016-01-01
• Series: Journal of Pathology Informatics
• Subjects: Big data; bioinformatics workflow; containerization; genomics
• Online Access: http://www.jpathinformatics.org/article.asp?issn=2153-3539;year=2016;volume=7;issue=1;spage=53;epage=53;aulast=Schulz

Background: The rapid acquisition of biological data and development of computationally intensive analyses has led to a need for novel approaches to software deployment. In particular, the complexity of common analytic tools for genomics makes them difficult to deploy and decreases the reproducibility of computational experiments. Methods: Recent technologies that allow for application virtualization, such as Docker, allow developers and bioinformaticians to isolate these applications and deploy secure, scalable platforms that have the potential to dramatically increase the efficiency of big data processing. Results: While limitations exist, this study demonstrates a successful implementation of a pipeline with several discrete software applications for the analysis of next-generation sequencing (NGS) data. Conclusions: With this approach, we significantly reduced the amount of time needed to perform clonal analysis from NGS data in acute myeloid leukemia.