Protective Effects of α-tocopherol on the Activity and Antioxidant Profile of Bovine Spermatozoa Subjected to Ferrous Ascorbate-Induced Oxidative Stress
As spermatozoa are highly vulnerable to oxidative stress development, in vitro antioxidants offer an additional line of defense to the male reproductive system against oxidative insults. α‑tocopherol (α-TOC) is the most abundant form of vitamin E identified in the seminal plasma and spermatozoa memb...
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doaj-71d6eb2fc731433099311540fcb6e2e52020-11-24T22:16:34ZengMendel University PressActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis1211-85162464-83102016-01-016441245125510.11118/actaun201664041245Protective Effects of α-tocopherol on the Activity and Antioxidant Profile of Bovine Spermatozoa Subjected to Ferrous Ascorbate-Induced Oxidative StressEva Tvrdá0Eva Tušimová1Katarína Zbyňovská2Tomáš Jambor3Norbert Lukáč4Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaAgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaAs spermatozoa are highly vulnerable to oxidative stress development, in vitro antioxidants offer an additional line of defense to the male reproductive system against oxidative insults. α‑tocopherol (α-TOC) is the most abundant form of vitamin E identified in the seminal plasma and spermatozoa membranes, being able to terminate numerous oxidative chain reactions causing substantial damage to biomolecules vital for sperm survival. This study was designed to shed more light on the in vitro effects of α‑tocopherol with respect to the vitality and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate-induced oxidative stress. Spermatozoa were washed out from 50 bovine ejaculates, suspended in 2.9 % sodium citrate and subjected to α-TOC treatment (10, 50, 100 and 500 μmol/L) in the presence or absence of ferrous ascorbate (FeAA; 150 μmol/L FeSO4 and 750 μmol/L ascorbic acid) during a 6h in vitro culture. Spermatozoa motion parameters were assessed using the SpermVision™ computer-aided sperm analysis (CASA) system. Cell viability was examined with the metabolic activity (MTT) assay, and the nitroblue-tetrazolium (NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the experiments in order to assess the intracellular activity of superoxide dismutase (SOD), catalase (CAT), as well as glutathione (GSH) and malondialdehyde (MDA) concentrations. Treatment with FeAA reduced both spermatozoa motility parameters (P < 0.001) as well as viability (P < 0.05 with respect to Time 0 h; P < 0.01 in case of Time 2 h and P < 0.001 in relation to Time 6 h), decreased the antioxidant parameters of the samples (P < 0.001 in case of SOD; P < 0.01 with respect to CAT and GSH) but increased the superoxide production (P < 0.01 in case of Time 0h and P < 0.001 with respect to Times 2 h and 6 h) and lipid peroxidation (P < 0.001). α‑TOC administration resulted in a preservation of the spermatozoa motility characteristics (P < 0.001 with respect to 500 μmol/L α-TOC), viability (P < 0.001 in case of 500 μmol/L α-TOC and P < 0.05 with respect to 100 μmol/L α-TOC) and antioxidant profile (P < 0.01 related to the impact of 500 μmol/L α-TOC on the SOD activity; P < 0.05 in relation to CAT; P < 0.01 with respect GSH; 100-500 μmol/L α-TOC), with 500 μmol/L α-TOC being the most effective. Our results suggest that α‑tocopherol possesses significant antioxidant properties that may prevent the deleterious effects caused by free radicals to spermatozoa, and extend the fertilization potential of male reproductive cells.https://acta.mendelu.cz/64/4/1245/α‑tocopherolspermatozoaoxidative stressferrous ascorbateantioxidantslipid peroxidation |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eva Tvrdá Eva Tušimová Katarína Zbyňovská Tomáš Jambor Norbert Lukáč |
spellingShingle |
Eva Tvrdá Eva Tušimová Katarína Zbyňovská Tomáš Jambor Norbert Lukáč Protective Effects of α-tocopherol on the Activity and Antioxidant Profile of Bovine Spermatozoa Subjected to Ferrous Ascorbate-Induced Oxidative Stress Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis α‑tocopherol spermatozoa oxidative stress ferrous ascorbate antioxidants lipid peroxidation |
author_facet |
Eva Tvrdá Eva Tušimová Katarína Zbyňovská Tomáš Jambor Norbert Lukáč |
author_sort |
Eva Tvrdá |
title |
Protective Effects of α-tocopherol on the Activity and Antioxidant Profile of Bovine Spermatozoa Subjected to Ferrous Ascorbate-Induced Oxidative Stress |
title_short |
Protective Effects of α-tocopherol on the Activity and Antioxidant Profile of Bovine Spermatozoa Subjected to Ferrous Ascorbate-Induced Oxidative Stress |
title_full |
Protective Effects of α-tocopherol on the Activity and Antioxidant Profile of Bovine Spermatozoa Subjected to Ferrous Ascorbate-Induced Oxidative Stress |
title_fullStr |
Protective Effects of α-tocopherol on the Activity and Antioxidant Profile of Bovine Spermatozoa Subjected to Ferrous Ascorbate-Induced Oxidative Stress |
title_full_unstemmed |
Protective Effects of α-tocopherol on the Activity and Antioxidant Profile of Bovine Spermatozoa Subjected to Ferrous Ascorbate-Induced Oxidative Stress |
title_sort |
protective effects of α-tocopherol on the activity and antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate-induced oxidative stress |
publisher |
Mendel University Press |
series |
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
issn |
1211-8516 2464-8310 |
publishDate |
2016-01-01 |
description |
As spermatozoa are highly vulnerable to oxidative stress development, in vitro antioxidants offer an additional line of defense to the male reproductive system against oxidative insults. α‑tocopherol (α-TOC) is the most abundant form of vitamin E identified in the seminal plasma and spermatozoa membranes, being able to terminate numerous oxidative chain reactions causing substantial damage to biomolecules vital for sperm survival. This study was designed to shed more light on the in vitro effects of α‑tocopherol with respect to the vitality and intracellular antioxidant profile of bovine spermatozoa subjected to ferrous ascorbate-induced oxidative stress. Spermatozoa were washed out from 50 bovine ejaculates, suspended in 2.9 % sodium citrate and subjected to α-TOC treatment (10, 50, 100 and 500 μmol/L) in the presence or absence of ferrous ascorbate (FeAA; 150 μmol/L FeSO4 and 750 μmol/L ascorbic acid) during a 6h in vitro culture. Spermatozoa motion parameters were assessed using the SpermVision™ computer-aided sperm analysis (CASA) system. Cell viability was examined with the metabolic activity (MTT) assay, and the nitroblue-tetrazolium (NBT) test was applied to quantify the intracellular superoxide formation. Cell lysates were prepared at the end of the experiments in order to assess the intracellular activity of superoxide dismutase (SOD), catalase (CAT), as well as glutathione (GSH) and malondialdehyde (MDA) concentrations. Treatment with FeAA reduced both spermatozoa motility parameters (P < 0.001) as well as viability (P < 0.05 with respect to Time 0 h; P < 0.01 in case of Time 2 h and P < 0.001 in relation to Time 6 h), decreased the antioxidant parameters of the samples (P < 0.001 in case of SOD; P < 0.01 with respect to CAT and GSH) but increased the superoxide production (P < 0.01 in case of Time 0h and P < 0.001 with respect to Times 2 h and 6 h) and lipid peroxidation (P < 0.001). α‑TOC administration resulted in a preservation of the spermatozoa motility characteristics (P < 0.001 with respect to 500 μmol/L α-TOC), viability (P < 0.001 in case of 500 μmol/L α-TOC and P < 0.05 with respect to 100 μmol/L α-TOC) and antioxidant profile (P < 0.01 related to the impact of 500 μmol/L α-TOC on the SOD activity; P < 0.05 in relation to CAT; P < 0.01 with respect GSH; 100-500 μmol/L α-TOC), with 500 μmol/L α-TOC being the most effective. Our results suggest that α‑tocopherol possesses significant antioxidant properties that may prevent the deleterious effects caused by free radicals to spermatozoa, and extend the fertilization potential of male reproductive cells. |
topic |
α‑tocopherol spermatozoa oxidative stress ferrous ascorbate antioxidants lipid peroxidation |
url |
https://acta.mendelu.cz/64/4/1245/ |
work_keys_str_mv |
AT evatvrda protectiveeffectsofatocopherolontheactivityandantioxidantprofileofbovinespermatozoasubjectedtoferrousascorbateinducedoxidativestress AT evatusimova protectiveeffectsofatocopherolontheactivityandantioxidantprofileofbovinespermatozoasubjectedtoferrousascorbateinducedoxidativestress AT katarinazbynovska protectiveeffectsofatocopherolontheactivityandantioxidantprofileofbovinespermatozoasubjectedtoferrousascorbateinducedoxidativestress AT tomasjambor protectiveeffectsofatocopherolontheactivityandantioxidantprofileofbovinespermatozoasubjectedtoferrousascorbateinducedoxidativestress AT norbertlukac protectiveeffectsofatocopherolontheactivityandantioxidantprofileofbovinespermatozoasubjectedtoferrousascorbateinducedoxidativestress |
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