Diversity and Metabolic Potential of Earthworm Gut Microbiota in Indo-Myanmar Biodiversity Hotspot
Earthworms are important members of the soil macrofauna that play a significant role in soil structure and fertility. However, there is scanty information on the earthworm gut microbial flora and their metabolic potential. In the present study, the diversity of the microbial community and their me...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Journal of Pure and Applied Microbiology
2020-06-01
|
Series: | Journal of Pure and Applied Microbiology |
Subjects: | |
Online Access: | https://microbiologyjournal.org/diversity-and-metabolic-potential-of-earthworm-gut-microbiota-in-indo-myanmar-biodiversity-hotspot/ |
id |
doaj-71e4346e360c4e3d999803e043cb1cf7 |
---|---|
record_format |
Article |
spelling |
doaj-71e4346e360c4e3d999803e043cb1cf72021-10-02T17:14:19ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2020-06-011421503151110.22207/JPAM.14.2.48Diversity and Metabolic Potential of Earthworm Gut Microbiota in Indo-Myanmar Biodiversity HotspotVabeiryureilai Mathipi0https://orcid.org/0000-0001-8708-3686Surajit De Mandal1https://orcid.org/0000-0002-0415-0183Zothansanga Chawngthu2Ruth Lalfelpuii3https://orcid.org/0000-0001-6772-6496Nachimuthu Senthil Kumar4https://orcid.org/0000-0002-1327-3150H. Lalthanzara5https://orcid.org/0000-0002-2625-9438Department of Biotechnology, Mizoram University, Aizawl - 796 004, Mizoram, India. Department of Biotechnology, Mizoram University, Aizawl - 796 004, Mizoram, India. Department of Zoology, Pachhunga University College, Aizawl - 796 001, Mizoram, India.Department of Biotechnology, Mizoram University, Aizawl - 796 004, Mizoram, India. Department of Biotechnology, Mizoram University, Aizawl - 796 004, Mizoram, India. Department of Zoology, Pachhunga University College, Aizawl - 796 001, Mizoram, India.Earthworms are important members of the soil macrofauna that play a significant role in soil structure and fertility. However, there is scanty information on the earthworm gut microbial flora and their metabolic potential. In the present study, the diversity of the microbial community and their metabolic potential from the gut content of four different earthworm species from Indo-Myanmar Biodiversity Hotspot were collected and identified by standard methods. The microbial diversity and their metabolic potential were assessed by high throughput sequencing of V3-V4 region of 16S rRNA using Illumina technology. Analysis of microbial diversity was performed by QIIME software package v.1.8.0 with their metabolic potential by PICRUSt (v1.1) software package. A total of 3,36,047 processed sequences were obtained that generated 3686 operational taxonomic units (OTUs). Major bacterial phyla identified were Proteobacteria (47.1%), Firmicutes (38.9%), Actinobacteria (6.3%), Bacteroidetes (3.6%) and Cyanobacteria (1.1%). The abundant genera were Lysinibacillus (26.9%), Acinetobacter (21.2%), Pseudomonas (4.7%), Bacillus (3.8%), Staphylococcus (3.5%), Stenotrophomonas (1.1%) and Ralstonia (1%). The functional annotation of the metagenome revealed abundance of bacterial community associated with amino acid, carbohydrate as well as energy metabolism. Furthermore, the presence of enzymes involved in the process of denitrification and methanogenesis were also identified. This study gives insight into the gut microbial composition and their putative functional roles in the gut of tropical hilly earthworms. The study on forest and garden soil earthworm gut microbiomes might help us understand the role of these organisms in their respective ecosystems.https://microbiologyjournal.org/diversity-and-metabolic-potential-of-earthworm-gut-microbiota-in-indo-myanmar-biodiversity-hotspot/earthworm gastrointestinalmicrobialmizoramnext generation sequencingsoil |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vabeiryureilai Mathipi Surajit De Mandal Zothansanga Chawngthu Ruth Lalfelpuii Nachimuthu Senthil Kumar H. Lalthanzara |
spellingShingle |
Vabeiryureilai Mathipi Surajit De Mandal Zothansanga Chawngthu Ruth Lalfelpuii Nachimuthu Senthil Kumar H. Lalthanzara Diversity and Metabolic Potential of Earthworm Gut Microbiota in Indo-Myanmar Biodiversity Hotspot Journal of Pure and Applied Microbiology earthworm gastrointestinal microbial mizoram next generation sequencing soil |
author_facet |
Vabeiryureilai Mathipi Surajit De Mandal Zothansanga Chawngthu Ruth Lalfelpuii Nachimuthu Senthil Kumar H. Lalthanzara |
author_sort |
Vabeiryureilai Mathipi |
title |
Diversity and Metabolic Potential of Earthworm Gut Microbiota in Indo-Myanmar Biodiversity Hotspot |
title_short |
Diversity and Metabolic Potential of Earthworm Gut Microbiota in Indo-Myanmar Biodiversity Hotspot |
title_full |
Diversity and Metabolic Potential of Earthworm Gut Microbiota in Indo-Myanmar Biodiversity Hotspot |
title_fullStr |
Diversity and Metabolic Potential of Earthworm Gut Microbiota in Indo-Myanmar Biodiversity Hotspot |
title_full_unstemmed |
Diversity and Metabolic Potential of Earthworm Gut Microbiota in Indo-Myanmar Biodiversity Hotspot |
title_sort |
diversity and metabolic potential of earthworm gut microbiota in indo-myanmar biodiversity hotspot |
publisher |
Journal of Pure and Applied Microbiology |
series |
Journal of Pure and Applied Microbiology |
issn |
0973-7510 2581-690X |
publishDate |
2020-06-01 |
description |
Earthworms are important members of the soil macrofauna that play a significant role in soil structure
and fertility. However, there is scanty information on the earthworm gut microbial flora and their
metabolic potential. In the present study, the diversity of the microbial community and their metabolic
potential from the gut content of four different earthworm species from Indo-Myanmar Biodiversity
Hotspot were collected and identified by standard methods. The microbial diversity and their metabolic
potential were assessed by high throughput sequencing of V3-V4 region of 16S rRNA using Illumina
technology. Analysis of microbial diversity was performed by QIIME software package v.1.8.0 with their
metabolic potential by PICRUSt (v1.1) software package. A total of 3,36,047 processed sequences were
obtained that generated 3686 operational taxonomic units (OTUs). Major bacterial phyla identified
were Proteobacteria (47.1%), Firmicutes (38.9%), Actinobacteria (6.3%), Bacteroidetes (3.6%) and
Cyanobacteria (1.1%). The abundant genera were Lysinibacillus (26.9%), Acinetobacter (21.2%),
Pseudomonas (4.7%), Bacillus (3.8%), Staphylococcus (3.5%), Stenotrophomonas (1.1%) and Ralstonia
(1%). The functional annotation of the metagenome revealed abundance of bacterial community
associated with amino acid, carbohydrate as well as energy metabolism. Furthermore, the presence
of enzymes involved in the process of denitrification and methanogenesis were also identified. This
study gives insight into the gut microbial composition and their putative functional roles in the gut
of tropical hilly earthworms. The study on forest and garden soil earthworm gut microbiomes might
help us understand the role of these organisms in their respective ecosystems. |
topic |
earthworm gastrointestinal microbial mizoram next generation sequencing soil |
url |
https://microbiologyjournal.org/diversity-and-metabolic-potential-of-earthworm-gut-microbiota-in-indo-myanmar-biodiversity-hotspot/ |
work_keys_str_mv |
AT vabeiryureilaimathipi diversityandmetabolicpotentialofearthwormgutmicrobiotainindomyanmarbiodiversityhotspot AT surajitdemandal diversityandmetabolicpotentialofearthwormgutmicrobiotainindomyanmarbiodiversityhotspot AT zothansangachawngthu diversityandmetabolicpotentialofearthwormgutmicrobiotainindomyanmarbiodiversityhotspot AT ruthlalfelpuii diversityandmetabolicpotentialofearthwormgutmicrobiotainindomyanmarbiodiversityhotspot AT nachimuthusenthilkumar diversityandmetabolicpotentialofearthwormgutmicrobiotainindomyanmarbiodiversityhotspot AT hlalthanzara diversityandmetabolicpotentialofearthwormgutmicrobiotainindomyanmarbiodiversityhotspot |
_version_ |
1716851967695781888 |