Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family
The two PIF1 family helicases in <i>Saccharomyces cerevisiae,</i> Rrm3, and ScPif1, associate with thousands of sites throughout the genome where they perform overlapping and distinct roles in telomere length maintenance, replication through non-histone proteins and G4 structures, laggin...
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doaj-71f23cf690594c9cb812dfa255baa7bb2020-11-25T02:37:07ZengMDPI AGGenes2073-44252020-02-0111222410.3390/genes11020224genes11020224Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase FamilyJulius Muellner0Kristina H. Schmidt1Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL 33620, USADepartment of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL 33620, USAThe two PIF1 family helicases in <i>Saccharomyces cerevisiae,</i> Rrm3, and ScPif1, associate with thousands of sites throughout the genome where they perform overlapping and distinct roles in telomere length maintenance, replication through non-histone proteins and G4 structures, lagging strand replication, replication fork convergence, the repair of DNA double-strand break ends, and transposable element mobility. ScPif1 and its fission yeast homolog Pfh1 also localize to mitochondria where they protect mitochondrial genome integrity. In addition to yeast serving as a model system for the rapid functional evaluation of human Pif1 variants, yeast cells lacking Rrm3 have proven useful for elucidating the cellular response to replication fork pausing at endogenous sites. Here, we review the increasingly important cellular functions of the yeast PIF1 helicases in maintaining genome integrity, and highlight recent advances in our understanding of their roles in facilitating fork progression through replisome barriers, their functional interactions with DNA repair, and replication stress response pathways.https://www.mdpi.com/2073-4425/11/2/224rrm3pif1pfh1g4 structuresdna replicationrdnareplication fork pausinggenome instability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julius Muellner Kristina H. Schmidt |
spellingShingle |
Julius Muellner Kristina H. Schmidt Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family Genes rrm3 pif1 pfh1 g4 structures dna replication rdna replication fork pausing genome instability |
author_facet |
Julius Muellner Kristina H. Schmidt |
author_sort |
Julius Muellner |
title |
Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family |
title_short |
Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family |
title_full |
Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family |
title_fullStr |
Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family |
title_full_unstemmed |
Yeast Genome Maintenance by the Multifunctional PIF1 DNA Helicase Family |
title_sort |
yeast genome maintenance by the multifunctional pif1 dna helicase family |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2020-02-01 |
description |
The two PIF1 family helicases in <i>Saccharomyces cerevisiae,</i> Rrm3, and ScPif1, associate with thousands of sites throughout the genome where they perform overlapping and distinct roles in telomere length maintenance, replication through non-histone proteins and G4 structures, lagging strand replication, replication fork convergence, the repair of DNA double-strand break ends, and transposable element mobility. ScPif1 and its fission yeast homolog Pfh1 also localize to mitochondria where they protect mitochondrial genome integrity. In addition to yeast serving as a model system for the rapid functional evaluation of human Pif1 variants, yeast cells lacking Rrm3 have proven useful for elucidating the cellular response to replication fork pausing at endogenous sites. Here, we review the increasingly important cellular functions of the yeast PIF1 helicases in maintaining genome integrity, and highlight recent advances in our understanding of their roles in facilitating fork progression through replisome barriers, their functional interactions with DNA repair, and replication stress response pathways. |
topic |
rrm3 pif1 pfh1 g4 structures dna replication rdna replication fork pausing genome instability |
url |
https://www.mdpi.com/2073-4425/11/2/224 |
work_keys_str_mv |
AT juliusmuellner yeastgenomemaintenancebythemultifunctionalpif1dnahelicasefamily AT kristinahschmidt yeastgenomemaintenancebythemultifunctionalpif1dnahelicasefamily |
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1724796629882503168 |