Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD)

Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD)

An 83-year old Alzheimer's disease (AD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry...

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Main Authors: Ying Wang, Jing Zhang, Ying Lei, Jian Zhao
Format: Article
Language:English
Published: Elsevier 2018-12-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506118302770
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spelling doaj-71f421c9e46a48e68fa8a1122379f0e12020-11-24T21:40:25ZengElsevierStem Cell Research1873-50612018-12-0133265268Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD)Ying Wang0Jing Zhang1Ying Lei2Jian Zhao3Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Tongji University School of Medicine, Shanghai, China; Lab of stem cell and neurodegeneration, Tongji University School of Medicine, Shanghai, China; Institutes of Biomedical Sciences, Fudan University, Shanghai, ChinaDepartment of Neurology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaStem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Tongji University School of Medicine, Shanghai, China; Corresponding authors at: Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Tongji University School of Medicine, Shanghai, China.Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Tongji University School of Medicine, Shanghai, China; Lab of stem cell and neurodegeneration, Tongji University School of Medicine, Shanghai, China; Corresponding authors at: Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Tongji University School of Medicine, Shanghai, China.An 83-year old Alzheimer's disease (AD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.http://www.sciencedirect.com/science/article/pii/S1873506118302770
collection DOAJ
language English
format Article
sources DOAJ
author Ying Wang
Jing Zhang
Ying Lei
Jian Zhao
spellingShingle Ying Wang
Jing Zhang
Ying Lei
Jian Zhao
Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD)
Stem Cell Research
author_facet Ying Wang
Jing Zhang
Ying Lei
Jian Zhao
author_sort Ying Wang
title Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD)
title_short Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD)
title_full Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD)
title_fullStr Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD)
title_full_unstemmed Establishment of TUSMi007-A, an induced pluripotent stem cell (iPSC) line from an 83-year old Chinese Han patient with Alzheimer's disease (AD)
title_sort establishment of tusmi007-a, an induced pluripotent stem cell (ipsc) line from an 83-year old chinese han patient with alzheimer's disease (ad)
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2018-12-01
description An 83-year old Alzheimer's disease (AD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.
url http://www.sciencedirect.com/science/article/pii/S1873506118302770
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AT jingzhang establishmentoftusmi007aaninducedpluripotentstemcellipsclinefroman83yearoldchinesehanpatientwithalzheimersdiseasead
AT yinglei establishmentoftusmi007aaninducedpluripotentstemcellipsclinefroman83yearoldchinesehanpatientwithalzheimersdiseasead
AT jianzhao establishmentoftusmi007aaninducedpluripotentstemcellipsclinefroman83yearoldchinesehanpatientwithalzheimersdiseasead
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