Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary
Objective: Functional in vitro studies are fundamental to understand the role of microRNAs, small non coding RNA molecules that function as post-transcriptional regulators, in cancer. The objective of this study was to determine the applicability of head and neck squamous cell carcinoma cell lines a...
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
2011-12-01
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doaj-71fd380bdedb430fa3fe3d508b2d1cc82020-11-25T02:46:27ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)1679-45082011-12-0194442448Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primaryFlavia Maziero AndreghettoMaria Fatima Guarizo KlingbeilRenata Machado SoaresRoberta SitnikDécio dos Santos Pinto JuniorMonica Beatriz MathorFabio Daumas NunesPatricia SeverinoObjective: Functional in vitro studies are fundamental to understand the role of microRNAs, small non coding RNA molecules that function as post-transcriptional regulators, in cancer. The objective of this study was to determine the applicability of head and neck squamous cell carcinoma cell lines and human oral keratinocytes as models for functional studies on microRNAs previously identified as deregulated in head and neck squamous cell carcinomas. Methods: The expression level of four microRNAs was assessed in cell lines and in primary cultures of oral keratinocytes using specific real-time polymerase chain reactions. The identity of oral squamous cell carcinoma cell lines was confirmed by means of STR (short tandem repeats) profiling. The possible impact of feeder-layer gene expression in global microRNA expression results from keratinocyte primary culture was also evaluated. Results: Significant differences in microRNA gene expression were observed among squamous cell carcinoma cell lines, particularly among cells lines from distinct subsites, as well as between primary culture of human keratinocytes and immortalized keratinocyte cell lines. Conclusions: Primary cultures of human keratinocytes and diverse tumor cell lines are relatively easy to obtain. However, each cell model possesses a characteristic phenotype; whereas one may be useful for a specific study, it may be inappropriate for another. Therefore, it is imperative that suitable cell lines are cautiously selected for functional studies in cancer.http://apps.einstein.br/revista/arquivos/PDF/2149-Einstein_v9n4_442-448_eng.pdfGene expressionKeratinocytesCarcinomasquamous cellHead and neck neoplasms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Flavia Maziero Andreghetto Maria Fatima Guarizo Klingbeil Renata Machado Soares Roberta Sitnik Décio dos Santos Pinto Junior Monica Beatriz Mathor Fabio Daumas Nunes Patricia Severino |
spellingShingle |
Flavia Maziero Andreghetto Maria Fatima Guarizo Klingbeil Renata Machado Soares Roberta Sitnik Décio dos Santos Pinto Junior Monica Beatriz Mathor Fabio Daumas Nunes Patricia Severino Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary Einstein (São Paulo) Gene expression Keratinocytes Carcinoma squamous cell Head and neck neoplasms |
author_facet |
Flavia Maziero Andreghetto Maria Fatima Guarizo Klingbeil Renata Machado Soares Roberta Sitnik Décio dos Santos Pinto Junior Monica Beatriz Mathor Fabio Daumas Nunes Patricia Severino |
author_sort |
Flavia Maziero Andreghetto |
title |
Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary |
title_short |
Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary |
title_full |
Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary |
title_fullStr |
Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary |
title_full_unstemmed |
Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary |
title_sort |
evaluation of microrna expression in head and neck squamous cell carcinoma cell lines and in primary |
publisher |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
series |
Einstein (São Paulo) |
issn |
1679-4508 |
publishDate |
2011-12-01 |
description |
Objective: Functional in vitro studies are fundamental to understand the role of microRNAs, small non coding RNA molecules that function as post-transcriptional regulators, in cancer. The objective of this study was to determine the applicability of head and neck squamous cell carcinoma cell lines and human oral keratinocytes as models for functional studies on microRNAs previously identified as deregulated in head and neck squamous cell carcinomas. Methods: The expression level of four microRNAs was assessed in cell lines and in primary cultures of oral keratinocytes using specific real-time polymerase chain reactions. The identity of oral squamous cell carcinoma cell lines was confirmed by means of STR (short tandem repeats) profiling. The possible impact of feeder-layer gene expression in global microRNA expression results from keratinocyte primary culture was also evaluated. Results: Significant differences in microRNA gene expression were observed among squamous cell carcinoma cell lines, particularly among cells lines from distinct subsites, as well as between primary culture of human keratinocytes and immortalized keratinocyte cell lines. Conclusions: Primary cultures of human keratinocytes and diverse tumor cell lines are relatively easy to obtain. However, each cell model possesses a characteristic phenotype; whereas one may be useful for a specific study, it may be inappropriate for another. Therefore, it is imperative that suitable cell lines are cautiously selected for functional studies in cancer. |
topic |
Gene expression Keratinocytes Carcinoma squamous cell Head and neck neoplasms |
url |
http://apps.einstein.br/revista/arquivos/PDF/2149-Einstein_v9n4_442-448_eng.pdf |
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