A Method to Identify p62's UBA Domain Interacting Proteins
<p>The UBA domain is a conserved sequence motif among polyubiquitin binding proteins. For the first time, we demonstrate a systematic, high throughput approach to identification of UBA domain-interacting proteins from a proteome-wide perspective. Using the rabbit reticulocyte lysate <it>...
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doaj-72038b7c14fd4626a331b349345dce6e2020-11-25T00:27:34ZengBMCBiological Procedures Online1480-92222003-01-015122823710.1251/bpo66A Method to Identify p62's UBA Domain Interacting Proteins Pridgeon Julia W.Geetha ThangiahWooten Marie W.<p>The UBA domain is a conserved sequence motif among polyubiquitin binding proteins. For the first time, we demonstrate a systematic, high throughput approach to identification of UBA domain-interacting proteins from a proteome-wide perspective. Using the rabbit reticulocyte lysate <it>in vitro</it> expression cloning system, we have successfully identified eleven proteins that interact with p62’s UBA domain, and the majority of the eleven proteins are associated with neurodegenerative disorders, such as Alzheimer’s disease. Therefore, p62 may play a novel regulatory role through its UBA domain. Our approach provides an easy route to the characterization of UBA domain interacting proteins and its application will unfold the important roles that the UBA domain plays.http://www.biologicalprocedures.com/bpo/arts/1/66/m66.htmUbiquitinAlzheimer Disease |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pridgeon Julia W. Geetha Thangiah Wooten Marie W. |
spellingShingle |
Pridgeon Julia W. Geetha Thangiah Wooten Marie W. A Method to Identify p62's UBA Domain Interacting Proteins Biological Procedures Online Ubiquitin Alzheimer Disease |
author_facet |
Pridgeon Julia W. Geetha Thangiah Wooten Marie W. |
author_sort |
Pridgeon Julia W. |
title |
A Method to Identify p62's UBA Domain Interacting Proteins |
title_short |
A Method to Identify p62's UBA Domain Interacting Proteins |
title_full |
A Method to Identify p62's UBA Domain Interacting Proteins |
title_fullStr |
A Method to Identify p62's UBA Domain Interacting Proteins |
title_full_unstemmed |
A Method to Identify p62's UBA Domain Interacting Proteins |
title_sort |
method to identify p62's uba domain interacting proteins |
publisher |
BMC |
series |
Biological Procedures Online |
issn |
1480-9222 |
publishDate |
2003-01-01 |
description |
<p>The UBA domain is a conserved sequence motif among polyubiquitin binding proteins. For the first time, we demonstrate a systematic, high throughput approach to identification of UBA domain-interacting proteins from a proteome-wide perspective. Using the rabbit reticulocyte lysate <it>in vitro</it> expression cloning system, we have successfully identified eleven proteins that interact with p62’s UBA domain, and the majority of the eleven proteins are associated with neurodegenerative disorders, such as Alzheimer’s disease. Therefore, p62 may play a novel regulatory role through its UBA domain. Our approach provides an easy route to the characterization of UBA domain interacting proteins and its application will unfold the important roles that the UBA domain plays. |
topic |
Ubiquitin Alzheimer Disease |
url |
http://www.biologicalprocedures.com/bpo/arts/1/66/m66.htm |
work_keys_str_mv |
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