Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals

Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals

Egia-Mendikute et al. develop a multiplexed flow cytometric bead array to simultaneously detect antibodies reactive to three immunogenic SARS-CoV-2 proteins. More sensitive than ELISA, they detected N-reactive antibodies in COVID-19-negative individuals with this assay, showing it to have superior p...

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Main Authors: Leire Egia-Mendikute, Alexandre Bosch, Endika Prieto-Fernández, So Young Lee, Borja Jiménez-Lasheras, Ana García del Río, Asier Antoñana-Vildosola, Chiara Bruzzone, Maider Bizkarguenaga, Nieves Embade, Rubén Gil-Redondo, María Luz Martínez-Chantar, Marcos López-Hoyos, Nicola G. A. Abrescia, José M. Mato, Óscar Millet, Asís Palazón
Format: Article
Language:English
Published: Nature Publishing Group 2021-04-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-021-02011-6
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spelling doaj-721616baa10e46b992979a7d09ffe6af2021-04-25T11:29:53ZengNature Publishing GroupCommunications Biology2399-36422021-04-014111010.1038/s42003-021-02011-6Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individualsLeire Egia-Mendikute0Alexandre Bosch1Endika Prieto-Fernández2So Young Lee3Borja Jiménez-Lasheras4Ana García del Río5Asier Antoñana-Vildosola6Chiara Bruzzone7Maider Bizkarguenaga8Nieves Embade9Rubén Gil-Redondo10María Luz Martínez-Chantar11Marcos López-Hoyos12Nicola G. A. Abrescia13José M. Mato14Óscar Millet15Asís Palazón16Cancer Immunology and Immunotherapy Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Cancer Immunology and Immunotherapy Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Cancer Immunology and Immunotherapy Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Cancer Immunology and Immunotherapy Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Cancer Immunology and Immunotherapy Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Cancer Immunology and Immunotherapy Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Cancer Immunology and Immunotherapy Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Precision Medicine and Metabolism Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Precision Medicine and Metabolism Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Precision Medicine and Metabolism Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Precision Medicine and Metabolism Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Liver Disease Laboratory, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Servicio Inmunología, Hospital Universitario Marqués de Valdecilla-IDIVAL, Facultad de Medicina, Universidad de CantabriaCentro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos IIIPrecision Medicine and Metabolism Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Precision Medicine and Metabolism Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Cancer Immunology and Immunotherapy Lab, CIC bioGUNE, Basque Research and Technology Alliance (BRTA)Egia-Mendikute et al. develop a multiplexed flow cytometric bead array to simultaneously detect antibodies reactive to three immunogenic SARS-CoV-2 proteins. More sensitive than ELISA, they detected N-reactive antibodies in COVID-19-negative individuals with this assay, showing it to have superior potential to detect low antibody titres compared to current gold standard serology methods.https://doi.org/10.1038/s42003-021-02011-6
collection DOAJ
language English
format Article
sources DOAJ
author Leire Egia-Mendikute
Alexandre Bosch
Endika Prieto-Fernández
So Young Lee
Borja Jiménez-Lasheras
Ana García del Río
Asier Antoñana-Vildosola
Chiara Bruzzone
Maider Bizkarguenaga
Nieves Embade
Rubén Gil-Redondo
María Luz Martínez-Chantar
Marcos López-Hoyos
Nicola G. A. Abrescia
José M. Mato
Óscar Millet
Asís Palazón
spellingShingle Leire Egia-Mendikute
Alexandre Bosch
Endika Prieto-Fernández
So Young Lee
Borja Jiménez-Lasheras
Ana García del Río
Asier Antoñana-Vildosola
Chiara Bruzzone
Maider Bizkarguenaga
Nieves Embade
Rubén Gil-Redondo
María Luz Martínez-Chantar
Marcos López-Hoyos
Nicola G. A. Abrescia
José M. Mato
Óscar Millet
Asís Palazón
Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
Communications Biology
author_facet Leire Egia-Mendikute
Alexandre Bosch
Endika Prieto-Fernández
So Young Lee
Borja Jiménez-Lasheras
Ana García del Río
Asier Antoñana-Vildosola
Chiara Bruzzone
Maider Bizkarguenaga
Nieves Embade
Rubén Gil-Redondo
María Luz Martínez-Chantar
Marcos López-Hoyos
Nicola G. A. Abrescia
José M. Mato
Óscar Millet
Asís Palazón
author_sort Leire Egia-Mendikute
title Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_short Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_full Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_fullStr Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_full_unstemmed Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
title_sort sensitive detection of sars-cov-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals
publisher Nature Publishing Group
series Communications Biology
issn 2399-3642
publishDate 2021-04-01
description Egia-Mendikute et al. develop a multiplexed flow cytometric bead array to simultaneously detect antibodies reactive to three immunogenic SARS-CoV-2 proteins. More sensitive than ELISA, they detected N-reactive antibodies in COVID-19-negative individuals with this assay, showing it to have superior potential to detect low antibody titres compared to current gold standard serology methods.
url https://doi.org/10.1038/s42003-021-02011-6
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