Evaluation of Human Telomeric G-Quadruplexes: The Influence of Overhanging Sequences on Quadruplex Stability and Folding
To date, various G-quadruplex structures have been reported in human telomeric sequences. Human telomeric repeats can form many topological structures depending on conditions and on base modification; parallel, antiparallel, and hybrid forms. The effect of salts and some specific ligands on conforma...
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doaj-727567139fda438fa5da046ad78c16ec2020-11-24T21:40:13ZengHindawi LimitedJournal of Nucleic Acids2090-021X2010-01-01201010.4061/2010/820356820356Evaluation of Human Telomeric G-Quadruplexes: The Influence of Overhanging Sequences on Quadruplex Stability and FoldingViktor Viglasky0Lubos Bauer1Katarina Tluckova2Peter Javorsky3Department of Biochemistry, Institute of Chemistry, Faculty of Sciences, P. J. Safarik University, Moyzesova 11, 04154 Kosice, SlovakiaDepartment of Biochemistry, Institute of Chemistry, Faculty of Sciences, P. J. Safarik University, Moyzesova 11, 04154 Kosice, SlovakiaDepartment of Biochemistry, Institute of Chemistry, Faculty of Sciences, P. J. Safarik University, Moyzesova 11, 04154 Kosice, SlovakiaInstitute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 04001 Košice, SlovakiaTo date, various G-quadruplex structures have been reported in human telomeric sequences. Human telomeric repeats can form many topological structures depending on conditions and on base modification; parallel, antiparallel, and hybrid forms. The effect of salts and some specific ligands on conformational switches between different conformers is known, but the influence of protruding sequences has rarely been discussed. In this paper, we analyze different quadruplex-forming oligomers derived from human telomeric sequences which contain 3′- and 5′-protruding nucleotides, not usually associated with the G-quadruplex motif. The study was performed using electrophoresis, CD, and UV spectroscopies. The major findings are (i) protruding nucleotides destabilize the G-quadruplex structure, and (ii) overhanging sequences influence the folding of the quadruplex.http://dx.doi.org/10.4061/2010/820356 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Viktor Viglasky Lubos Bauer Katarina Tluckova Peter Javorsky |
spellingShingle |
Viktor Viglasky Lubos Bauer Katarina Tluckova Peter Javorsky Evaluation of Human Telomeric G-Quadruplexes: The Influence of Overhanging Sequences on Quadruplex Stability and Folding Journal of Nucleic Acids |
author_facet |
Viktor Viglasky Lubos Bauer Katarina Tluckova Peter Javorsky |
author_sort |
Viktor Viglasky |
title |
Evaluation of Human Telomeric G-Quadruplexes: The Influence of Overhanging Sequences on Quadruplex Stability and Folding |
title_short |
Evaluation of Human Telomeric G-Quadruplexes: The Influence of Overhanging Sequences on Quadruplex Stability and Folding |
title_full |
Evaluation of Human Telomeric G-Quadruplexes: The Influence of Overhanging Sequences on Quadruplex Stability and Folding |
title_fullStr |
Evaluation of Human Telomeric G-Quadruplexes: The Influence of Overhanging Sequences on Quadruplex Stability and Folding |
title_full_unstemmed |
Evaluation of Human Telomeric G-Quadruplexes: The Influence of Overhanging Sequences on Quadruplex Stability and Folding |
title_sort |
evaluation of human telomeric g-quadruplexes: the influence of overhanging sequences on quadruplex stability and folding |
publisher |
Hindawi Limited |
series |
Journal of Nucleic Acids |
issn |
2090-021X |
publishDate |
2010-01-01 |
description |
To date, various G-quadruplex structures have been reported in human telomeric sequences. Human telomeric repeats can form many topological structures depending on conditions and on base modification; parallel, antiparallel, and hybrid forms. The effect of salts and some specific ligands on conformational switches between different conformers is known, but the influence of protruding sequences has rarely been discussed. In this paper, we analyze different quadruplex-forming oligomers derived from human telomeric sequences which contain 3′- and 5′-protruding nucleotides, not usually associated with the G-quadruplex motif. The study was performed using electrophoresis, CD, and UV spectroscopies. The major findings are (i) protruding nucleotides destabilize the G-quadruplex structure, and (ii) overhanging sequences influence the folding of the quadruplex. |
url |
http://dx.doi.org/10.4061/2010/820356 |
work_keys_str_mv |
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