Evaluation of a Possible Synergistic Effect of Meglumine Antimoniate with Paromomycin, Miltefosine or Allopurinol on in Vitro Susceptibility of Leishmania tropica Resistant Isolate
Background: Pentavalent antimonials are still the first choice treatment for leishmaniasis, but with low efficacy and resistance is emerging. In the present study, the effect of meglumine antimoniate (MA, Glucantime) combined with paromomy-cin, miltefosine or allopurinol on in vitro susceptibil...
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doaj-727616dc1f244acb880922f3a3e519e32021-04-02T13:04:15ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2013-09-0183Evaluation of a Possible Synergistic Effect of Meglumine Antimoniate with Paromomycin, Miltefosine or Allopurinol on in Vitro Susceptibility of Leishmania tropica Resistant IsolateTahereh Rezaei Riabi0Iraj Sharifi1Akram Miramin Mohammadi2Ali Khamesipour3Maryam Hakimi Parizi4Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.Center for Research &Training in Skin Diseases & Leprosy, Tehran University of Medical Sciences, Tehran, Iran.Center for Research &Training in Skin Diseases & Leprosy, Tehran University of Medical Sciences, Tehran, Iran.Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran. Background: Pentavalent antimonials are still the first choice treatment for leishmaniasis, but with low efficacy and resistance is emerging. In the present study, the effect of meglumine antimoniate (MA, Glucantime) combined with paromomy-cin, miltefosine or allopurinol on in vitro susceptibility of Leishmania tropica resistant isolate was evaluated. Method: The drugs were obtained from commercial sources and diluents of each drug in medium were prepared on the day of experiment. J774 A.1 murine macro-phage cell lines were attached to the cultured on slide and incubated at 37 0C with 5% CO2 for 24 h. Then the stationary phase promastigotes were added to the cells and after 4 hrs of incubation different concentrations of MA, paromomycin, miltefosine or allopurinol were added and incubated for an additional of 72 h. Then the slides were dried and fixed with methanol, stained by Giemsa and studied under a light microscope. Drug activity was evaluated by assessing the macrophage infec-tion rate and the number of amastigotes per infected macrophage was done by ex-amining 100 macrophages. The experiment was done in triplicates. Result : Various concentrations of MA along with paromomycin, miltefosine or allopurinol significantly inhibited (P<0.01) the proliferation of L. tropica amastigote stage in the macrophage cell line as compared with MA alone or positive control. Conclusion: Combination of Glucantime with paromomycin, miltefosine or allo-purinol showed a synergistic effect on the clinical isolate of L. tropica in vitro. Use of combination therapy is a new hope and a logical basis for therapy of the patients with cutaneous leishmaniasis. Further investigations are needed to evaluate the therapeutic effects of these drugs on the CL patients. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/478In vitroLeishmania tropicaResistanceTreatment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tahereh Rezaei Riabi Iraj Sharifi Akram Miramin Mohammadi Ali Khamesipour Maryam Hakimi Parizi |
spellingShingle |
Tahereh Rezaei Riabi Iraj Sharifi Akram Miramin Mohammadi Ali Khamesipour Maryam Hakimi Parizi Evaluation of a Possible Synergistic Effect of Meglumine Antimoniate with Paromomycin, Miltefosine or Allopurinol on in Vitro Susceptibility of Leishmania tropica Resistant Isolate Iranian Journal of Parasitology In vitro Leishmania tropica Resistance Treatment |
author_facet |
Tahereh Rezaei Riabi Iraj Sharifi Akram Miramin Mohammadi Ali Khamesipour Maryam Hakimi Parizi |
author_sort |
Tahereh Rezaei Riabi |
title |
Evaluation of a Possible Synergistic Effect of Meglumine Antimoniate with Paromomycin, Miltefosine or Allopurinol on in Vitro Susceptibility of Leishmania tropica Resistant Isolate |
title_short |
Evaluation of a Possible Synergistic Effect of Meglumine Antimoniate with Paromomycin, Miltefosine or Allopurinol on in Vitro Susceptibility of Leishmania tropica Resistant Isolate |
title_full |
Evaluation of a Possible Synergistic Effect of Meglumine Antimoniate with Paromomycin, Miltefosine or Allopurinol on in Vitro Susceptibility of Leishmania tropica Resistant Isolate |
title_fullStr |
Evaluation of a Possible Synergistic Effect of Meglumine Antimoniate with Paromomycin, Miltefosine or Allopurinol on in Vitro Susceptibility of Leishmania tropica Resistant Isolate |
title_full_unstemmed |
Evaluation of a Possible Synergistic Effect of Meglumine Antimoniate with Paromomycin, Miltefosine or Allopurinol on in Vitro Susceptibility of Leishmania tropica Resistant Isolate |
title_sort |
evaluation of a possible synergistic effect of meglumine antimoniate with paromomycin, miltefosine or allopurinol on in vitro susceptibility of leishmania tropica resistant isolate |
publisher |
Tehran University of Medical Sciences |
series |
Iranian Journal of Parasitology |
issn |
1735-7020 2008-238X |
publishDate |
2013-09-01 |
description |
Background: Pentavalent antimonials are still the first choice treatment for leishmaniasis, but with low efficacy and resistance is emerging. In the present study, the effect of meglumine antimoniate (MA, Glucantime) combined with paromomy-cin, miltefosine or allopurinol on in vitro susceptibility of Leishmania tropica resistant isolate was evaluated.
Method: The drugs were obtained from commercial sources and diluents of each drug in medium were prepared on the day of experiment. J774 A.1 murine macro-phage cell lines were attached to the cultured on slide and incubated at 37 0C with 5% CO2 for 24 h. Then the stationary phase promastigotes were added to the cells and after 4 hrs of incubation different concentrations of MA, paromomycin, miltefosine or allopurinol were added and incubated for an additional of 72 h. Then the slides were dried and fixed with methanol, stained by Giemsa and studied under a light microscope. Drug activity was evaluated by assessing the macrophage infec-tion rate and the number of amastigotes per infected macrophage was done by ex-amining 100 macrophages. The experiment was done in triplicates.
Result : Various concentrations of MA along with paromomycin, miltefosine or allopurinol significantly inhibited (P<0.01) the proliferation of L. tropica amastigote stage in the macrophage cell line as compared with MA alone or positive control.
Conclusion: Combination of Glucantime with paromomycin, miltefosine or allo-purinol showed a synergistic effect on the clinical isolate of L. tropica in vitro. Use of combination therapy is a new hope and a logical basis for therapy of the patients with cutaneous leishmaniasis. Further investigations are needed to evaluate the therapeutic effects of these drugs on the CL patients.
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topic |
In vitro Leishmania tropica Resistance Treatment |
url |
https://ijpa.tums.ac.ir/index.php/ijpa/article/view/478 |
work_keys_str_mv |
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