Preparation of DPPC liposomes using probe-tip sonication: Investigating intrinsic factors affecting temperature phase transitions
Liposomes are an important tool and have gained much attention for their promise as an effective means of delivering small therapeutic compounds to targeted sites. In an effort to establish an effective method to produce liposomes from the lipid, dipalmitoyl-phosphatidylcholine or DPPC, we have foun...
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doaj-7285ab6cee3e49fa851fd6248d2d6efd2020-11-25T03:10:11ZengElsevierBiochemistry and Biophysics Reports2405-58082020-07-0122Preparation of DPPC liposomes using probe-tip sonication: Investigating intrinsic factors affecting temperature phase transitionsMonica D. Rieth0Andrew Lozano1Corresponding author.; Department of Chemistry, Southern Illinois University Edwardsville, 44 South Circle Drive, Box 1652, Science West, Edwardsville, IL, 62026, USADepartment of Chemistry, Southern Illinois University Edwardsville, 44 South Circle Drive, Box 1652, Science West, Edwardsville, IL, 62026, USALiposomes are an important tool and have gained much attention for their promise as an effective means of delivering small therapeutic compounds to targeted sites. In an effort to establish an effective method to produce liposomes from the lipid, dipalmitoyl-phosphatidylcholine or DPPC, we have found important aspects that must be taken into consideration. Here, we used probe-tip sonication to prepare liposomes on a batch scale. During this process we uncovered interesting steps in their preparation that altered the thermodynamic properties and phase transitions of the resulting liposome mixtures. Using differential scanning calorimetry to assess this we found that increasing the sonication time had the most dramatic effect on our sample, producing almost an entirely separate phase transition relative to the main phase transition. This result is consistent with reports from the current literature. We also highlight a smaller transition, which we attribute to traces of unincorporated lipid that seems to gradually disappear as the total lipid concentration decreases. Overall, sonication is an effective means of producing liposomes, but we cannot assert this method is optimal in producing them with precise physical properties. Here we highlight the physical effects at play during this process.http://www.sciencedirect.com/science/article/pii/S240558082030073Xdipalmitoylphosphatidylcholineliposomesphase transitionsdifferential scanning calorimetry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Monica D. Rieth Andrew Lozano |
spellingShingle |
Monica D. Rieth Andrew Lozano Preparation of DPPC liposomes using probe-tip sonication: Investigating intrinsic factors affecting temperature phase transitions Biochemistry and Biophysics Reports dipalmitoylphosphatidylcholine liposomes phase transitions differential scanning calorimetry |
author_facet |
Monica D. Rieth Andrew Lozano |
author_sort |
Monica D. Rieth |
title |
Preparation of DPPC liposomes using probe-tip sonication: Investigating intrinsic factors affecting temperature phase transitions |
title_short |
Preparation of DPPC liposomes using probe-tip sonication: Investigating intrinsic factors affecting temperature phase transitions |
title_full |
Preparation of DPPC liposomes using probe-tip sonication: Investigating intrinsic factors affecting temperature phase transitions |
title_fullStr |
Preparation of DPPC liposomes using probe-tip sonication: Investigating intrinsic factors affecting temperature phase transitions |
title_full_unstemmed |
Preparation of DPPC liposomes using probe-tip sonication: Investigating intrinsic factors affecting temperature phase transitions |
title_sort |
preparation of dppc liposomes using probe-tip sonication: investigating intrinsic factors affecting temperature phase transitions |
publisher |
Elsevier |
series |
Biochemistry and Biophysics Reports |
issn |
2405-5808 |
publishDate |
2020-07-01 |
description |
Liposomes are an important tool and have gained much attention for their promise as an effective means of delivering small therapeutic compounds to targeted sites. In an effort to establish an effective method to produce liposomes from the lipid, dipalmitoyl-phosphatidylcholine or DPPC, we have found important aspects that must be taken into consideration. Here, we used probe-tip sonication to prepare liposomes on a batch scale. During this process we uncovered interesting steps in their preparation that altered the thermodynamic properties and phase transitions of the resulting liposome mixtures. Using differential scanning calorimetry to assess this we found that increasing the sonication time had the most dramatic effect on our sample, producing almost an entirely separate phase transition relative to the main phase transition. This result is consistent with reports from the current literature. We also highlight a smaller transition, which we attribute to traces of unincorporated lipid that seems to gradually disappear as the total lipid concentration decreases. Overall, sonication is an effective means of producing liposomes, but we cannot assert this method is optimal in producing them with precise physical properties. Here we highlight the physical effects at play during this process. |
topic |
dipalmitoylphosphatidylcholine liposomes phase transitions differential scanning calorimetry |
url |
http://www.sciencedirect.com/science/article/pii/S240558082030073X |
work_keys_str_mv |
AT monicadrieth preparationofdppcliposomesusingprobetipsonicationinvestigatingintrinsicfactorsaffectingtemperaturephasetransitions AT andrewlozano preparationofdppcliposomesusingprobetipsonicationinvestigatingintrinsicfactorsaffectingtemperaturephasetransitions |
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