Role of TRPM7 channels in hyperglycemia-mediated injury of vascular endothelial cells.
This study investigated the change of transient receptor potential melastatin 7 (TRPM7) expression by high glucose and its role in hyperglycemia induced injury of vascular endothelial cells. Human umbilical vein endothelial cells (HUVECs) were incubated in the presence or absence of high concentrati...
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doaj-72a9133650fd4b038f93a8002e93140e2020-11-25T02:27:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7954010.1371/journal.pone.0079540Role of TRPM7 channels in hyperglycemia-mediated injury of vascular endothelial cells.Huawei SunTiandong LengZhao ZengXiuren GaoKoichi InoueZhi-Gang XiongThis study investigated the change of transient receptor potential melastatin 7 (TRPM7) expression by high glucose and its role in hyperglycemia induced injury of vascular endothelial cells. Human umbilical vein endothelial cells (HUVECs) were incubated in the presence or absence of high concentrations of D-glucose (HG) for 72 h. RT-PCR, Real-time PCR, Western blotting, Immunofluorescence staining and whole-cell patch-clamp recordings showed that TRPM7 mRNA, TRPM7 protein expression and TRPM7-like currents were increased in HUVECs following exposure to HG. In contrast to D-glucose, exposure of HUVECs to high concentrations of L-glucose had no effect. HG increased reactive oxygen species (ROS) generation, cytotoxicity and decreased endothelial nitric oxide synthase protein expression, which could be attenuated by knockdown of TRPM7 with TRPM7 siRNA. The protective effect of silencing TRPM7 against HG induced endothelial injury was abolished by U0126, an inhibitor of the extracellular signal-regulated kinase signaling pathway. These observations suggest that TRPM7 channels play an important role in hyperglycemia-induced injury of vascular endothelial cells.http://europepmc.org/articles/PMC3815131?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huawei Sun Tiandong Leng Zhao Zeng Xiuren Gao Koichi Inoue Zhi-Gang Xiong |
spellingShingle |
Huawei Sun Tiandong Leng Zhao Zeng Xiuren Gao Koichi Inoue Zhi-Gang Xiong Role of TRPM7 channels in hyperglycemia-mediated injury of vascular endothelial cells. PLoS ONE |
author_facet |
Huawei Sun Tiandong Leng Zhao Zeng Xiuren Gao Koichi Inoue Zhi-Gang Xiong |
author_sort |
Huawei Sun |
title |
Role of TRPM7 channels in hyperglycemia-mediated injury of vascular endothelial cells. |
title_short |
Role of TRPM7 channels in hyperglycemia-mediated injury of vascular endothelial cells. |
title_full |
Role of TRPM7 channels in hyperglycemia-mediated injury of vascular endothelial cells. |
title_fullStr |
Role of TRPM7 channels in hyperglycemia-mediated injury of vascular endothelial cells. |
title_full_unstemmed |
Role of TRPM7 channels in hyperglycemia-mediated injury of vascular endothelial cells. |
title_sort |
role of trpm7 channels in hyperglycemia-mediated injury of vascular endothelial cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
This study investigated the change of transient receptor potential melastatin 7 (TRPM7) expression by high glucose and its role in hyperglycemia induced injury of vascular endothelial cells. Human umbilical vein endothelial cells (HUVECs) were incubated in the presence or absence of high concentrations of D-glucose (HG) for 72 h. RT-PCR, Real-time PCR, Western blotting, Immunofluorescence staining and whole-cell patch-clamp recordings showed that TRPM7 mRNA, TRPM7 protein expression and TRPM7-like currents were increased in HUVECs following exposure to HG. In contrast to D-glucose, exposure of HUVECs to high concentrations of L-glucose had no effect. HG increased reactive oxygen species (ROS) generation, cytotoxicity and decreased endothelial nitric oxide synthase protein expression, which could be attenuated by knockdown of TRPM7 with TRPM7 siRNA. The protective effect of silencing TRPM7 against HG induced endothelial injury was abolished by U0126, an inhibitor of the extracellular signal-regulated kinase signaling pathway. These observations suggest that TRPM7 channels play an important role in hyperglycemia-induced injury of vascular endothelial cells. |
url |
http://europepmc.org/articles/PMC3815131?pdf=render |
work_keys_str_mv |
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