Antimicrobial and Antibiofilm Properties of Graphene Oxide on <i>Enterococcus faecalis</i>
The aim of this study was to evaluate the antibacterial properties of graphene oxide (GO) against <i>Enterococcus faecalis </i>in vitro conditions and when used to coat dentin surface to prevent <i>E. faecalis</i> adhesion. The ATCC strain of <i>E. faecalis</i> 29...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-10-01
|
Series: | Antibiotics |
Subjects: | |
Online Access: | https://www.mdpi.com/2079-6382/9/10/692 |
id |
doaj-72def952ff0c46fc9144fc7f0c022225 |
---|---|
record_format |
Article |
spelling |
doaj-72def952ff0c46fc9144fc7f0c0222252020-11-25T02:48:09ZengMDPI AGAntibiotics2079-63822020-10-01969269210.3390/antibiotics9100692Antimicrobial and Antibiofilm Properties of Graphene Oxide on <i>Enterococcus faecalis</i>Cecilia Martini0Francesca Longo1Raffaella Castagnola2Luca Marigo3Nicola Maria Grande4Massimo Cordaro5Margherita Cacaci6Massimiliano Papi7Valentina Palmieri8Francesca Bugli9Maurizio Sanguinetti10Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 20123 Rome, ItalyUOC Odontoiatria Generale e Ortodonzia, Dipartimento Scienze dell’Invecchiamento, Neurologiche, Ortopediche e della Testa Collo. Fondazione Policlinico Universitario “A. Gemelli” IRCCS, 00168 Rome, ItalyUOC Odontoiatria Generale e Ortodonzia, Dipartimento Scienze dell’Invecchiamento, Neurologiche, Ortopediche e della Testa Collo. Fondazione Policlinico Universitario “A. Gemelli” IRCCS, 00168 Rome, ItalyUOC Odontoiatria Generale e Ortodonzia, Dipartimento Scienze dell’Invecchiamento, Neurologiche, Ortopediche e della Testa Collo. Fondazione Policlinico Universitario “A. Gemelli” IRCCS, 00168 Rome, ItalyUOC Odontoiatria Generale e Ortodonzia, Dipartimento Scienze dell’Invecchiamento, Neurologiche, Ortopediche e della Testa Collo. Fondazione Policlinico Universitario “A. Gemelli” IRCCS, 00168 Rome, ItalyUOC Odontoiatria Generale e Ortodonzia, Dipartimento Scienze dell’Invecchiamento, Neurologiche, Ortopediche e della Testa Collo. Fondazione Policlinico Universitario “A. Gemelli” IRCCS, 00168 Rome, ItalyDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 20123 Rome, ItalyFondazione Policlinico Universitario “A. Gemelli” IRCCS, 00168 Rome, ItalyFondazione Policlinico Universitario “A. Gemelli” IRCCS, 00168 Rome, ItalyDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 20123 Rome, ItalyDipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 20123 Rome, ItalyThe aim of this study was to evaluate the antibacterial properties of graphene oxide (GO) against <i>Enterococcus faecalis </i>in vitro conditions and when used to coat dentin surface to prevent <i>E. faecalis</i> adhesion. The ATCC strain of <i>E. faecalis</i> 29212 has been used to perform a viability test. The pellet was suspended in ultrapure water, NaCl, PBS buffer, CaCl<sub>2</sub> and MgCl<sub>2</sub>, Luria−Bertani broth solutions. The viability was evaluated by the colony forming unit counting method. Atomic force microscopy images and the measure of surface zeta potential variation were analyzed. Dentin discs were covered with a film of GO (<i>n </i>= 15) or were not treated (<i>n </i>= 15). Bacterial suspension was added to each sample of dentine discs and microbial counts were calculated. Statistically significant differences between two groups were assessed by a two-tailed unpaired <i>t</i>-test. Bacteria cell morphology was investigated with scanning electron microscopy. The highest growth inhibition was obtained in ddH<sub>2</sub>O and CaCl<sub>2</sub> solution while, in PBS and NaCl, GO had poor antibacterial efficacy with a growth enhancing effect in the latter. GO on dentin discs demonstrated high antibacterial activity. GO film has demonstrated acceptable adhesion properties to root dentin and a role in the inhibition of bacterial film proliferation and biofilm formation.https://www.mdpi.com/2079-6382/9/10/692antimicrobial propertiesbiofilmgraphene oxide<i>Enterococcus faecalis</i>radicular dentin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cecilia Martini Francesca Longo Raffaella Castagnola Luca Marigo Nicola Maria Grande Massimo Cordaro Margherita Cacaci Massimiliano Papi Valentina Palmieri Francesca Bugli Maurizio Sanguinetti |
spellingShingle |
Cecilia Martini Francesca Longo Raffaella Castagnola Luca Marigo Nicola Maria Grande Massimo Cordaro Margherita Cacaci Massimiliano Papi Valentina Palmieri Francesca Bugli Maurizio Sanguinetti Antimicrobial and Antibiofilm Properties of Graphene Oxide on <i>Enterococcus faecalis</i> Antibiotics antimicrobial properties biofilm graphene oxide <i>Enterococcus faecalis</i> radicular dentin |
author_facet |
Cecilia Martini Francesca Longo Raffaella Castagnola Luca Marigo Nicola Maria Grande Massimo Cordaro Margherita Cacaci Massimiliano Papi Valentina Palmieri Francesca Bugli Maurizio Sanguinetti |
author_sort |
Cecilia Martini |
title |
Antimicrobial and Antibiofilm Properties of Graphene Oxide on <i>Enterococcus faecalis</i> |
title_short |
Antimicrobial and Antibiofilm Properties of Graphene Oxide on <i>Enterococcus faecalis</i> |
title_full |
Antimicrobial and Antibiofilm Properties of Graphene Oxide on <i>Enterococcus faecalis</i> |
title_fullStr |
Antimicrobial and Antibiofilm Properties of Graphene Oxide on <i>Enterococcus faecalis</i> |
title_full_unstemmed |
Antimicrobial and Antibiofilm Properties of Graphene Oxide on <i>Enterococcus faecalis</i> |
title_sort |
antimicrobial and antibiofilm properties of graphene oxide on <i>enterococcus faecalis</i> |
publisher |
MDPI AG |
series |
Antibiotics |
issn |
2079-6382 |
publishDate |
2020-10-01 |
description |
The aim of this study was to evaluate the antibacterial properties of graphene oxide (GO) against <i>Enterococcus faecalis </i>in vitro conditions and when used to coat dentin surface to prevent <i>E. faecalis</i> adhesion. The ATCC strain of <i>E. faecalis</i> 29212 has been used to perform a viability test. The pellet was suspended in ultrapure water, NaCl, PBS buffer, CaCl<sub>2</sub> and MgCl<sub>2</sub>, Luria−Bertani broth solutions. The viability was evaluated by the colony forming unit counting method. Atomic force microscopy images and the measure of surface zeta potential variation were analyzed. Dentin discs were covered with a film of GO (<i>n </i>= 15) or were not treated (<i>n </i>= 15). Bacterial suspension was added to each sample of dentine discs and microbial counts were calculated. Statistically significant differences between two groups were assessed by a two-tailed unpaired <i>t</i>-test. Bacteria cell morphology was investigated with scanning electron microscopy. The highest growth inhibition was obtained in ddH<sub>2</sub>O and CaCl<sub>2</sub> solution while, in PBS and NaCl, GO had poor antibacterial efficacy with a growth enhancing effect in the latter. GO on dentin discs demonstrated high antibacterial activity. GO film has demonstrated acceptable adhesion properties to root dentin and a role in the inhibition of bacterial film proliferation and biofilm formation. |
topic |
antimicrobial properties biofilm graphene oxide <i>Enterococcus faecalis</i> radicular dentin |
url |
https://www.mdpi.com/2079-6382/9/10/692 |
work_keys_str_mv |
AT ceciliamartini antimicrobialandantibiofilmpropertiesofgrapheneoxideonienterococcusfaecalisi AT francescalongo antimicrobialandantibiofilmpropertiesofgrapheneoxideonienterococcusfaecalisi AT raffaellacastagnola antimicrobialandantibiofilmpropertiesofgrapheneoxideonienterococcusfaecalisi AT lucamarigo antimicrobialandantibiofilmpropertiesofgrapheneoxideonienterococcusfaecalisi AT nicolamariagrande antimicrobialandantibiofilmpropertiesofgrapheneoxideonienterococcusfaecalisi AT massimocordaro antimicrobialandantibiofilmpropertiesofgrapheneoxideonienterococcusfaecalisi AT margheritacacaci antimicrobialandantibiofilmpropertiesofgrapheneoxideonienterococcusfaecalisi AT massimilianopapi antimicrobialandantibiofilmpropertiesofgrapheneoxideonienterococcusfaecalisi AT valentinapalmieri antimicrobialandantibiofilmpropertiesofgrapheneoxideonienterococcusfaecalisi AT francescabugli antimicrobialandantibiofilmpropertiesofgrapheneoxideonienterococcusfaecalisi AT mauriziosanguinetti antimicrobialandantibiofilmpropertiesofgrapheneoxideonienterococcusfaecalisi |
_version_ |
1724749531823734784 |