Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine Center
The venom proteome of Siamese Russell’s viper from Taiwan, alongside complementary in vivo lethality neutralization assay and in vitro third-generation antivenomics assessment of the preclinical efficacy of the homologous antivenom manufactured in Taiwan CDC’s Vaccine Center, are...
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doaj-72e696b0ace7402f8d28f4015066eef62020-11-25T00:55:53ZengMDPI AGTropical Medicine and Infectious Disease2414-63662018-06-01326610.3390/tropicalmed3020066tropicalmed3020066Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine CenterLibia Sanz0Sarai Quesada-Bernat1Pei Yu Chen2Cheng Dow Lee3Jen Ron Chiang4Juan J. Calvete5Evolutionary and Translational Venomics Laboratory, Consejo Superior de Investigaciones Científicas (CSIC), 46010 Valencia, SpainEvolutionary and Translational Venomics Laboratory, Consejo Superior de Investigaciones Científicas (CSIC), 46010 Valencia, SpainCenter for Research, Diagnostics and Vaccine Development, Centers for Disease Control (CDC), 11561 Taipei, TaiwanCenter for Research, Diagnostics and Vaccine Development, Centers for Disease Control (CDC), 11561 Taipei, TaiwanCenter for Research, Diagnostics and Vaccine Development, Centers for Disease Control (CDC), 11561 Taipei, TaiwanEvolutionary and Translational Venomics Laboratory, Consejo Superior de Investigaciones Científicas (CSIC), 46010 Valencia, SpainThe venom proteome of Siamese Russell’s viper from Taiwan, alongside complementary in vivo lethality neutralization assay and in vitro third-generation antivenomics assessment of the preclinical efficacy of the homologous antivenom manufactured in Taiwan CDC’s Vaccine Center, are here reported. Taiwanese Russell’s viper venom proteome comprised 25 distinct gene products, with the heterodimeric PLA2 viperotoxin-F representing the most abundant toxin (47.5% of total venom proteome). Coagulation FV-activating serine proteinase (RVV-V, 14%), the PIV-SVMP activator of FX (RVV-FX, 8.5%), and less abundant toxins from nine protein families, make up its venom proteome. Venom composition-pathology correlations of D. siamensis envenomings in Taiwan are discussed. The lethal effect of Taiwanese D. siamensis venom was 0.47 mg/g mouse. Antivenomics-guided assessment of the toxin recognition landscape of the Taiwanese Russell’s viper antivenom, in conjunction with complementary in vivo neutralization analysis, informed the antivenom’s maximal toxin immunorecognition ability (14 mg total venom proteins/vial), neutralization capacity (6.5 mg venom/vial), and relative content of lethality neutralizing antibodies (46.5% of the toxin-binding F(ab’)2 antibodies). The antigenomic analysis also revealed suboptimal aspects of the CDC-Taiwan antivenom. Strategies to improve them are suggested.http://www.mdpi.com/2414-6366/3/2/66Daboia siamensisvenomicsanti-Siamese Russell’s viper antivenomTaiwan CDC Vaccine Centerthird-generation antivenomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Libia Sanz Sarai Quesada-Bernat Pei Yu Chen Cheng Dow Lee Jen Ron Chiang Juan J. Calvete |
spellingShingle |
Libia Sanz Sarai Quesada-Bernat Pei Yu Chen Cheng Dow Lee Jen Ron Chiang Juan J. Calvete Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine Center Tropical Medicine and Infectious Disease Daboia siamensis venomics anti-Siamese Russell’s viper antivenom Taiwan CDC Vaccine Center third-generation antivenomics |
author_facet |
Libia Sanz Sarai Quesada-Bernat Pei Yu Chen Cheng Dow Lee Jen Ron Chiang Juan J. Calvete |
author_sort |
Libia Sanz |
title |
Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine Center |
title_short |
Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine Center |
title_full |
Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine Center |
title_fullStr |
Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine Center |
title_full_unstemmed |
Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine Center |
title_sort |
translational venomics: third-generation antivenomics of anti-siamese russell’s viper, daboia siamensis, antivenom manufactured in taiwan cdc’s vaccine center |
publisher |
MDPI AG |
series |
Tropical Medicine and Infectious Disease |
issn |
2414-6366 |
publishDate |
2018-06-01 |
description |
The venom proteome of Siamese Russell’s viper from Taiwan, alongside complementary in vivo lethality neutralization assay and in vitro third-generation antivenomics assessment of the preclinical efficacy of the homologous antivenom manufactured in Taiwan CDC’s Vaccine Center, are here reported. Taiwanese Russell’s viper venom proteome comprised 25 distinct gene products, with the heterodimeric PLA2 viperotoxin-F representing the most abundant toxin (47.5% of total venom proteome). Coagulation FV-activating serine proteinase (RVV-V, 14%), the PIV-SVMP activator of FX (RVV-FX, 8.5%), and less abundant toxins from nine protein families, make up its venom proteome. Venom composition-pathology correlations of D. siamensis envenomings in Taiwan are discussed. The lethal effect of Taiwanese D. siamensis venom was 0.47 mg/g mouse. Antivenomics-guided assessment of the toxin recognition landscape of the Taiwanese Russell’s viper antivenom, in conjunction with complementary in vivo neutralization analysis, informed the antivenom’s maximal toxin immunorecognition ability (14 mg total venom proteins/vial), neutralization capacity (6.5 mg venom/vial), and relative content of lethality neutralizing antibodies (46.5% of the toxin-binding F(ab’)2 antibodies). The antigenomic analysis also revealed suboptimal aspects of the CDC-Taiwan antivenom. Strategies to improve them are suggested. |
topic |
Daboia siamensis venomics anti-Siamese Russell’s viper antivenom Taiwan CDC Vaccine Center third-generation antivenomics |
url |
http://www.mdpi.com/2414-6366/3/2/66 |
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