PCR-RFLP Is a Useful Tool to Distinguish between C. Dubliniensis and C. Albicans in Cancer Patients in Iran

• Main Authors: Zohreh Saltanatpouri, Tahereh Shokohi, Mohammad Bagher Hashemi Soteh, Mohammad Taghi Hedayati
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2010-06-01
• Series: International Journal of Hematology-Oncology and Stem Cell Research
• Subjects: PCR- RFLP; C .dubliniensis; C. albicans; BlnI
• Online Access: https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/243

Introduction: Candida dubliniensis and C. albicans are very similar in morphology and phenotypic characteristics. Approximation of this yeast species has caused major problems in identifying these two correctly. Materials and Methods: To distinguish among sixty yeast clinical isolates from patients with cancer, polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) was done. Results: PCR-RFLP of the ITS region showed different patterns between Candida dubliniensis and C. albicans after digestion with enzymes BlnI. All of the Clinical isolates were diagnosed as C .albicans.  The results were confirmed by sequencing and RAPD-PCR. Conclusion:PCR-RFLP would be a useful and applicable technique in clinical laboratories for discrimination of C. albicans and C. dubliniensis.