Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression

Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression

The Lateolabrax maculatus is an important marine fish in China that can adapt to a wide range of water salinities. The gill is the main organ involved in fish osmoregulation and in this study, a cDNA library of the L. maculatus gill was constructed to understand gill function in Lateolabrax maculatu...

Full description

Bibliographic Details
Main Authors: Yawei Shen, Yudong He, Yanhui Bi, Jianpeng Chen, Ziwei Zhao, Jun Li, Xiaowu Chen
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2019-11-01
Series:Aquaculture and Fisheries
Online Access:http://www.sciencedirect.com/science/article/pii/S2468550X18302302
id doaj-72ed78f9a2fd42468dd3bebf735318a2
record_format Article
spelling doaj-72ed78f9a2fd42468dd3bebf735318a22021-02-02T06:02:43ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2019-11-0146247254Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expressionYawei Shen0Yudong He1Yanhui Bi2Jianpeng Chen3Ziwei Zhao4Jun Li5Xiaowu Chen6National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, ChinaShanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, 201306, ChinaShanghai Engineering Research Center of Aquaculture, Shanghai, 201306, ChinaShanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, 201306, ChinaShanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai, 201306, ChinaCAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 26000, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 26000, ChinaNational Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Corresponding author. National Demonstration Center for Experimental Fisheries, Science Education, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai, 201306, China.The Lateolabrax maculatus is an important marine fish in China that can adapt to a wide range of water salinities. The gill is the main organ involved in fish osmoregulation and in this study, a cDNA library of the L. maculatus gill was constructed to understand gill function in Lateolabrax maculatus and it adaptation to salinity. A total raw data of 5.3G base pairs was obtained, followed removal of low-quality and linker DNA sequences and it yielded a total of 23,038,590 clean reads. After splicing and clustering, 31,976 unigenes were obtained. The average transcript length was 1327 bp and N50 was 2057 bp. In comparisons with other tissues analysis of the gill transcriptome revealed a high level of relative expression of apolipoprotein E, CD74, keratin, type I cytoskeleton 13, carbonic anhydrase, betaine-homocysteine S-methyltransferase, and aquaporin 3 (AQP3) genes. The L. maculatus aqp3 (Lm-aqp3) gene was specific for the gills Lm-aqp3 and this gene has a typical transmembrane structure of six alpha helices and a water channel domain. Phylogenetic analysis indicated that Lm-aqp3 clusters with the homologue genes from Labrus bergylta and Dicentrarchus labrax. Quantitative RT-PCR confirmed that Lm-aqp3 was highly abundant in the gills and low expressed in other tissues (stomach, liver, brain, spleen and testis). Analysis of Lm-aqp3 in freshwater aquaculture fish revealed that gene expression was significantly statistical higher than in seawater (20 ppt) fish, suggesting that Lm-aqp3 may play an important role in water metabolism and osmotic pressure regulation in L. maculatus. Keywords: Lateolabrax maculatus, Gill, Transcriptome, Aquaporin 3http://www.sciencedirect.com/science/article/pii/S2468550X18302302
collection DOAJ
language English
format Article
sources DOAJ
author Yawei Shen
Yudong He
Yanhui Bi
Jianpeng Chen
Ziwei Zhao
Jun Li
Xiaowu Chen
spellingShingle Yawei Shen
Yudong He
Yanhui Bi
Jianpeng Chen
Ziwei Zhao
Jun Li
Xiaowu Chen
Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression
Aquaculture and Fisheries
author_facet Yawei Shen
Yudong He
Yanhui Bi
Jianpeng Chen
Ziwei Zhao
Jun Li
Xiaowu Chen
author_sort Yawei Shen
title Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression
title_short Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression
title_full Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression
title_fullStr Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression
title_full_unstemmed Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression
title_sort transcriptome analysis of gill from lateolabrax maculatus and aqp3 gene expression
publisher KeAi Communications Co., Ltd.
series Aquaculture and Fisheries
issn 2468-550X
publishDate 2019-11-01
description The Lateolabrax maculatus is an important marine fish in China that can adapt to a wide range of water salinities. The gill is the main organ involved in fish osmoregulation and in this study, a cDNA library of the L. maculatus gill was constructed to understand gill function in Lateolabrax maculatus and it adaptation to salinity. A total raw data of 5.3G base pairs was obtained, followed removal of low-quality and linker DNA sequences and it yielded a total of 23,038,590 clean reads. After splicing and clustering, 31,976 unigenes were obtained. The average transcript length was 1327 bp and N50 was 2057 bp. In comparisons with other tissues analysis of the gill transcriptome revealed a high level of relative expression of apolipoprotein E, CD74, keratin, type I cytoskeleton 13, carbonic anhydrase, betaine-homocysteine S-methyltransferase, and aquaporin 3 (AQP3) genes. The L. maculatus aqp3 (Lm-aqp3) gene was specific for the gills Lm-aqp3 and this gene has a typical transmembrane structure of six alpha helices and a water channel domain. Phylogenetic analysis indicated that Lm-aqp3 clusters with the homologue genes from Labrus bergylta and Dicentrarchus labrax. Quantitative RT-PCR confirmed that Lm-aqp3 was highly abundant in the gills and low expressed in other tissues (stomach, liver, brain, spleen and testis). Analysis of Lm-aqp3 in freshwater aquaculture fish revealed that gene expression was significantly statistical higher than in seawater (20 ppt) fish, suggesting that Lm-aqp3 may play an important role in water metabolism and osmotic pressure regulation in L. maculatus. Keywords: Lateolabrax maculatus, Gill, Transcriptome, Aquaporin 3
url http://www.sciencedirect.com/science/article/pii/S2468550X18302302
work_keys_str_mv AT yaweishen transcriptomeanalysisofgillfromlateolabraxmaculatusandaqp3geneexpression
AT yudonghe transcriptomeanalysisofgillfromlateolabraxmaculatusandaqp3geneexpression
AT yanhuibi transcriptomeanalysisofgillfromlateolabraxmaculatusandaqp3geneexpression
AT jianpengchen transcriptomeanalysisofgillfromlateolabraxmaculatusandaqp3geneexpression
AT ziweizhao transcriptomeanalysisofgillfromlateolabraxmaculatusandaqp3geneexpression
AT junli transcriptomeanalysisofgillfromlateolabraxmaculatusandaqp3geneexpression
AT xiaowuchen transcriptomeanalysisofgillfromlateolabraxmaculatusandaqp3geneexpression
_version_ 1724302136611700736

Similar Items