Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction
Epigenetic information is transmitted from mother to daughter cells through mitosis. Here, the authors isolate native chromosomes from metaphase-arrested cells and perform LC-MS/MS to identify chromosome-bound proteins in pluripotent stem cells during mitosis and reveal that PRC2, DNA methylation an...
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2020-08-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-020-17823-z |
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doaj-73176d0dba7348dcb968ea5de1aa87c62021-08-22T11:41:16ZengNature Publishing GroupNature Communications2041-17232020-08-0111111510.1038/s41467-020-17823-zIdentifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compactionDounia Djeghloul0Bhavik Patel1Holger Kramer2Andrew Dimond3Chad Whilding4Karen Brown5Anne-Céline Kohler6Amelie Feytout7Nicolas Veland8James Elliott9Tanmay A. M. Bharat10Abul K. Tarafder11Jan Löwe12Bee L. Ng13Ya Guo14Jacky Guy15Miles K. Huseyin16Robert J. Klose17Matthias Merkenschlager18Amanda G. Fisher19Lymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusFlow Cytometry Facility, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusBiological Mass Spectrometry and Proteomics Facility, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusLymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusMicroscopy Facility, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusLymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusLymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusLymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusLymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusFlow Cytometry Facility, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusSir William Dunn School of Pathology, University of OxfordSir William Dunn School of Pathology, University of OxfordMRC Laboratory of Molecular BiologyWellcome Sanger Institute, Wellcome Genome CampusLymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusThe Wellcome Centre for Cell Biology, University of EdinburghDepartment of Biochemistry, University of OxfordDepartment of Biochemistry, University of OxfordLymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusLymphocyte Development Group, MRC London Institute of Medical Sciences, Imperial College London, Hammersmith Hospital CampusEpigenetic information is transmitted from mother to daughter cells through mitosis. Here, the authors isolate native chromosomes from metaphase-arrested cells and perform LC-MS/MS to identify chromosome-bound proteins in pluripotent stem cells during mitosis and reveal that PRC2, DNA methylation and Mecp2 are required to maintain chromosome compaction.https://doi.org/10.1038/s41467-020-17823-z |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dounia Djeghloul Bhavik Patel Holger Kramer Andrew Dimond Chad Whilding Karen Brown Anne-Céline Kohler Amelie Feytout Nicolas Veland James Elliott Tanmay A. M. Bharat Abul K. Tarafder Jan Löwe Bee L. Ng Ya Guo Jacky Guy Miles K. Huseyin Robert J. Klose Matthias Merkenschlager Amanda G. Fisher |
spellingShingle |
Dounia Djeghloul Bhavik Patel Holger Kramer Andrew Dimond Chad Whilding Karen Brown Anne-Céline Kohler Amelie Feytout Nicolas Veland James Elliott Tanmay A. M. Bharat Abul K. Tarafder Jan Löwe Bee L. Ng Ya Guo Jacky Guy Miles K. Huseyin Robert J. Klose Matthias Merkenschlager Amanda G. Fisher Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction Nature Communications |
author_facet |
Dounia Djeghloul Bhavik Patel Holger Kramer Andrew Dimond Chad Whilding Karen Brown Anne-Céline Kohler Amelie Feytout Nicolas Veland James Elliott Tanmay A. M. Bharat Abul K. Tarafder Jan Löwe Bee L. Ng Ya Guo Jacky Guy Miles K. Huseyin Robert J. Klose Matthias Merkenschlager Amanda G. Fisher |
author_sort |
Dounia Djeghloul |
title |
Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction |
title_short |
Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction |
title_full |
Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction |
title_fullStr |
Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction |
title_full_unstemmed |
Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction |
title_sort |
identifying proteins bound to native mitotic esc chromosomes reveals chromatin repressors are important for compaction |
publisher |
Nature Publishing Group |
series |
Nature Communications |
issn |
2041-1723 |
publishDate |
2020-08-01 |
description |
Epigenetic information is transmitted from mother to daughter cells through mitosis. Here, the authors isolate native chromosomes from metaphase-arrested cells and perform LC-MS/MS to identify chromosome-bound proteins in pluripotent stem cells during mitosis and reveal that PRC2, DNA methylation and Mecp2 are required to maintain chromosome compaction. |
url |
https://doi.org/10.1038/s41467-020-17823-z |
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