Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin.
Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effect...
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doaj-732bc80f7b7e48f59514ef88a6fa7bff2020-11-25T02:04:48ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352015-01-0197e000391710.1371/journal.pntd.0003917Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin.Arnone NithichanonDarawan RinchaiAlessandro GoriPatricia LassauxClaudio PeriOscar Conchillio-SoléMario Ferrer-NavarroLouise J GourlayMarco NardiniJordi VilaXavier DauraGiorgio ColomboMartino BolognesiGanjana LertmemonkolchaiBurkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequence-based epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components.http://europepmc.org/articles/PMC4519301?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Arnone Nithichanon Darawan Rinchai Alessandro Gori Patricia Lassaux Claudio Peri Oscar Conchillio-Solé Mario Ferrer-Navarro Louise J Gourlay Marco Nardini Jordi Vila Xavier Daura Giorgio Colombo Martino Bolognesi Ganjana Lertmemonkolchai |
spellingShingle |
Arnone Nithichanon Darawan Rinchai Alessandro Gori Patricia Lassaux Claudio Peri Oscar Conchillio-Solé Mario Ferrer-Navarro Louise J Gourlay Marco Nardini Jordi Vila Xavier Daura Giorgio Colombo Martino Bolognesi Ganjana Lertmemonkolchai Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin. PLoS Neglected Tropical Diseases |
author_facet |
Arnone Nithichanon Darawan Rinchai Alessandro Gori Patricia Lassaux Claudio Peri Oscar Conchillio-Solé Mario Ferrer-Navarro Louise J Gourlay Marco Nardini Jordi Vila Xavier Daura Giorgio Colombo Martino Bolognesi Ganjana Lertmemonkolchai |
author_sort |
Arnone Nithichanon |
title |
Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin. |
title_short |
Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin. |
title_full |
Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin. |
title_fullStr |
Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin. |
title_full_unstemmed |
Sequence- and Structure-Based Immunoreactive Epitope Discovery for Burkholderia pseudomallei Flagellin. |
title_sort |
sequence- and structure-based immunoreactive epitope discovery for burkholderia pseudomallei flagellin. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Neglected Tropical Diseases |
issn |
1935-2727 1935-2735 |
publishDate |
2015-01-01 |
description |
Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequence-based epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components. |
url |
http://europepmc.org/articles/PMC4519301?pdf=render |
work_keys_str_mv |
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