Biased diversity metrics revealed by bacterial 16S pyrotags derived from different primer sets.

In recent years, PCR-based pyrosequencing of 16S rRNA genes has continuously increased our understanding of complex microbial communities in various environments of the Earth. However, there is always concern on the potential biases of diversity determination using different 16S rRNA gene primer set...

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Bibliographic Details
Main Authors: Lin Cai, Lin Ye, Amy Hin Yan Tong, Si Lok, Tong Zhang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3544912?pdf=render

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