| Summary: | In the present work, sheets of Papyrus (<i>Cyperus papyrus</i> L.), manufactured by lamination from strips pre-treated with different treatments, were evaluated for their technological and fungal infestation properties (<i>Aspergillus flavus</i> AFl375, <i>A. niger</i> Ani245 and <i>Colletotrichum gloeosporioides</i> Cgl311). The results showed that the highest values of tensile strength, tear strength, burst index and double-fold number were observed in papyrus sheets produced from strips treated with nano-cellulose (0.25%), dimethyl sulfoxide (DMSO 10%), Tylose (0.25%) and nano-cellulose (0.5%), with values of 98.90 N·m/g, 2343.67 mN·m<sup>2</sup>/g, 1162 kpa·m<sup>2</sup>/g and 8.33, respectively. The percentage of brightness ranged from 49.7% (strips treated with KOH 2% + 100 mL NaClO) to 9.6% (strips treated with <i>Eucalyptus camaldulensis</i> bark extract 2%), while the percentage of darkness ranged from 99.86% (strips treated with <i>Salix babylonica</i> leaf extract 2% or <i>E. camaldulensis</i> bark extract 0.5%) to 67.26% (strips treated with NaOH (2%) + 100 mL NaClO). From the SEM examination, sheets produced from treated strips with extracts from <i>P. rigida</i> and <i>E. camaldulensis</i> or <i>S. babylonica</i> showed no growths of <i>A. flavus</i> and <i>C. gloeosporioides</i>. Additionally, other pre-treatments, such as Nano-cellulose+Tylose 0.5% (1:1 <i>v</i>/<i>v</i>) and Tylose 0.5%, were also found to have no growth of <i>A. niger</i>. In conclusion, strips pre-treated with nanomaterials and extracts were enhanced in terms of the technological and antifungal properties of produced Papyrus sheets, respectively.
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