Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis
A more effective vaccine against tuberculosis (TB) is urgently needed. Based on its high genetic homology with Mycobacterium tuberculosis (Mtb), the nonpathogenic mycobacteria, Mycobacterium smegmatis (Ms), could be an attractive source of potential antigens to be included in such a vaccine. We eval...
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doaj-73633dce2dc0473288e9fdd1bd00d5dd2020-11-24T20:42:11ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/273129273129Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary TuberculosisMaria de los Angeles García0Reinier Borrero1Maria E. Lanio2Yanely Tirado3Nadine Alvarez4Alina Puig5Alicia Aguilar6Liem Canet7Dulce Mata Espinoza8Jorge Barrios Payán9María Elena Sarmiento10Rogelio Hernández-Pando11Mohd-Nor Norazmi12Armando Acosta13Instituto Finlay, 11600 Havana, CubaInstituto Finlay, 11600 Havana, CubaCenter for Protein Studies, Faculty of Biology, Havana University, 10400 Havana, CubaInstituto Finlay, 11600 Havana, CubaInstituto Finlay, 11600 Havana, CubaInstituto Finlay, 11600 Havana, CubaInstituto Finlay, 11600 Havana, CubaCenter for Protein Studies, Faculty of Biology, Havana University, 10400 Havana, CubaInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14000 México, DF, MexicoInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14000 México, DF, MexicoInstituto Finlay, 11600 Havana, CubaInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14000 México, DF, MexicoSchool of Health Sciences, Universiti Sains Malaysia, MalaysiaInstituto Finlay, 11600 Havana, CubaA more effective vaccine against tuberculosis (TB) is urgently needed. Based on its high genetic homology with Mycobacterium tuberculosis (Mtb), the nonpathogenic mycobacteria, Mycobacterium smegmatis (Ms), could be an attractive source of potential antigens to be included in such a vaccine. We evaluated the capability of lipid-based preparations obtained from Ms to provide a protective response in Balb/c mice after challenge with Mtb H37Rv strain. The intratracheal model of progressive pulmonary TB was used to assess the level of protection in terms of bacterial load as well as the pathological changes in the lungs of immunized Balb/c mice following challenge with Mtb. Mice immunized with the lipid-based preparation from Ms either adjuvanted with Alum (LMs-AL) or nonadjuvanted (LMs) showed significant reductions in bacterial load (P<0.01) compared to the negative control group (animals immunized with phosphate buffered saline (PBS)). Both lipid formulations showed the same level of protection as Bacille Calmette and Guerin (BCG). Regarding the pathologic changes in the lungs, mice immunized with both lipid formulations showed less pneumonic area when compared with the PBS group (P<0.01) and showed similar results compared with the BCG group. These findings suggest the potential of LMs as a promising vaccine candidate against TB.http://dx.doi.org/10.1155/2014/273129 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria de los Angeles García Reinier Borrero Maria E. Lanio Yanely Tirado Nadine Alvarez Alina Puig Alicia Aguilar Liem Canet Dulce Mata Espinoza Jorge Barrios Payán María Elena Sarmiento Rogelio Hernández-Pando Mohd-Nor Norazmi Armando Acosta |
spellingShingle |
Maria de los Angeles García Reinier Borrero Maria E. Lanio Yanely Tirado Nadine Alvarez Alina Puig Alicia Aguilar Liem Canet Dulce Mata Espinoza Jorge Barrios Payán María Elena Sarmiento Rogelio Hernández-Pando Mohd-Nor Norazmi Armando Acosta Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis BioMed Research International |
author_facet |
Maria de los Angeles García Reinier Borrero Maria E. Lanio Yanely Tirado Nadine Alvarez Alina Puig Alicia Aguilar Liem Canet Dulce Mata Espinoza Jorge Barrios Payán María Elena Sarmiento Rogelio Hernández-Pando Mohd-Nor Norazmi Armando Acosta |
author_sort |
Maria de los Angeles García |
title |
Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis |
title_short |
Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis |
title_full |
Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis |
title_fullStr |
Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis |
title_full_unstemmed |
Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis |
title_sort |
protective effect of a lipid-based preparation from mycobacterium smegmatis in a murine model of progressive pulmonary tuberculosis |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
A more effective vaccine against tuberculosis (TB) is urgently needed. Based on its high genetic homology with Mycobacterium tuberculosis (Mtb), the nonpathogenic mycobacteria, Mycobacterium smegmatis (Ms), could be an attractive source of potential antigens to be included in such a vaccine. We evaluated the capability of lipid-based preparations obtained from Ms to provide a protective response in Balb/c mice after challenge with Mtb H37Rv strain. The intratracheal model of progressive pulmonary TB was used to assess the level of protection in terms of bacterial load as well as the pathological changes in the lungs of immunized Balb/c mice following challenge with Mtb. Mice immunized with the lipid-based preparation from Ms either adjuvanted with Alum (LMs-AL) or nonadjuvanted (LMs) showed significant reductions in bacterial load (P<0.01) compared to the negative control group (animals immunized with phosphate buffered saline (PBS)). Both lipid formulations showed the same level of protection as Bacille Calmette and Guerin (BCG). Regarding the pathologic changes in the lungs, mice immunized with both lipid formulations showed less pneumonic area when compared with the PBS group (P<0.01) and showed similar results compared with the BCG group. These findings suggest the potential of LMs as a promising vaccine candidate against TB. |
url |
http://dx.doi.org/10.1155/2014/273129 |
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