Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean

Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean

The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: Gmβ-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was ext...

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Bibliographic Details
Main Authors: Renata Stolf-Moreira, Eliana Gertrudes de Macedo Lemos, Ricardo Vilela Abdelnoor, Magda Aparecida Beneventi, Amanda Alves Paiva Rolla, Selma dos Santos Pereira, Maria Cristina Neves de Oliveira, Alexandre Lima Nepomuceno, Francismar Corrêa Marcelino-Guimarães
Format: Article
Language:English
Published: Embrapa Informação Tecnológica 2011-01-01
Series:Pesquisa Agropecuária Brasileira
Subjects:
Glycine max
estabilidade de expressão
RT-qPCR
déficit hídrico
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000100008&lng=en&tlng=en

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