Polycomb Responds to Low Levels of Transcription
How is Polycomb (Pc), a eukaryotic negative regulator of transcription, targeted to specific mammalian genes? Our genome-wide analysis of the Pc mark H3K27me3 in murine cells revealed that Pc is preferentially associated with CpG island promoters of genes that are transcribed at a low level and less...
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doaj-7385f301de9a4ff1867367603739db4b2020-11-25T01:02:28ZengElsevierCell Reports2211-12472017-07-0120478579310.1016/j.celrep.2017.06.076Polycomb Responds to Low Levels of TranscriptionGeorgina Berrozpe0Gene O. Bryant1Katherine Warpinski2Dan Spagna3Santosh Narayan4Shivangi Shah5Mark Ptashne6Molecular Biology Program, Memorial Sloan Kettering Cancer Center, 430 East 67th Street, New York, NY 10065, USAMolecular Biology Program, Memorial Sloan Kettering Cancer Center, 430 East 67th Street, New York, NY 10065, USAMolecular Biology Program, Memorial Sloan Kettering Cancer Center, 430 East 67th Street, New York, NY 10065, USAMolecular Biology Program, Memorial Sloan Kettering Cancer Center, 430 East 67th Street, New York, NY 10065, USAMolecular Biology Program, Memorial Sloan Kettering Cancer Center, 430 East 67th Street, New York, NY 10065, USAMolecular Biology Program, Memorial Sloan Kettering Cancer Center, 430 East 67th Street, New York, NY 10065, USAMolecular Biology Program, Memorial Sloan Kettering Cancer Center, 430 East 67th Street, New York, NY 10065, USAHow is Polycomb (Pc), a eukaryotic negative regulator of transcription, targeted to specific mammalian genes? Our genome-wide analysis of the Pc mark H3K27me3 in murine cells revealed that Pc is preferentially associated with CpG island promoters of genes that are transcribed at a low level and less so with promoters of genes that are either silent or more highly expressed. Studies of the CpG island promoter of the Kit gene demonstrate that Pc is largely absent when the gene is silent in myeloid cells, as well as when the gene is highly expressed in mast cells. Manipulations that increase transcription in the former case, and reduce it in the latter, increase Pc occupancy. The average negative effect of Pc, we infer, is about 2-fold. We suggest possible biological roles for such negative effects and propose a mechanism by which Pc might be recruited to weakly transcribed genes.http://www.sciencedirect.com/science/article/pii/S2211124717309075PolycombKit genegenome-widetranscriptionCpG island promotersCRISPRshRAD21enhancers |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Georgina Berrozpe Gene O. Bryant Katherine Warpinski Dan Spagna Santosh Narayan Shivangi Shah Mark Ptashne |
spellingShingle |
Georgina Berrozpe Gene O. Bryant Katherine Warpinski Dan Spagna Santosh Narayan Shivangi Shah Mark Ptashne Polycomb Responds to Low Levels of Transcription Cell Reports Polycomb Kit gene genome-wide transcription CpG island promoters CRISPR shRAD21 enhancers |
author_facet |
Georgina Berrozpe Gene O. Bryant Katherine Warpinski Dan Spagna Santosh Narayan Shivangi Shah Mark Ptashne |
author_sort |
Georgina Berrozpe |
title |
Polycomb Responds to Low Levels of Transcription |
title_short |
Polycomb Responds to Low Levels of Transcription |
title_full |
Polycomb Responds to Low Levels of Transcription |
title_fullStr |
Polycomb Responds to Low Levels of Transcription |
title_full_unstemmed |
Polycomb Responds to Low Levels of Transcription |
title_sort |
polycomb responds to low levels of transcription |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2017-07-01 |
description |
How is Polycomb (Pc), a eukaryotic negative regulator of transcription, targeted to specific mammalian genes? Our genome-wide analysis of the Pc mark H3K27me3 in murine cells revealed that Pc is preferentially associated with CpG island promoters of genes that are transcribed at a low level and less so with promoters of genes that are either silent or more highly expressed. Studies of the CpG island promoter of the Kit gene demonstrate that Pc is largely absent when the gene is silent in myeloid cells, as well as when the gene is highly expressed in mast cells. Manipulations that increase transcription in the former case, and reduce it in the latter, increase Pc occupancy. The average negative effect of Pc, we infer, is about 2-fold. We suggest possible biological roles for such negative effects and propose a mechanism by which Pc might be recruited to weakly transcribed genes. |
topic |
Polycomb Kit gene genome-wide transcription CpG island promoters CRISPR shRAD21 enhancers |
url |
http://www.sciencedirect.com/science/article/pii/S2211124717309075 |
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